Polycation-coated polyanion microspheres of urease for urea hydrolysis

Artificial Cells, Blood Substitutes, and Immobilization Biotechnology

Volumn 28, Issue 1, 2000, Pages 95-111

  Elçin, A Eser ^a   Elçin, Y Murat ^a  

^a ANKARA UNIVERSITY   (Turkey)

Author keywords

Alginate; Carboxymethylcellulose; Chitosan; Immobilization; Microspher e; Microsphere coating; Urea hydrolysis; Urease

Indexed keywords

ACTIVATION ENERGY; CHITIN; ENZYME IMMOBILIZATION; HYDROLYSIS; PH; POLYSACCHARIDES; TEMPERATURE; UREA;

ALGINATE; CARBOXYMETHYLCELLULOSE; CHITOSAN; UREASE;

ENZYMES;

ALGINIC ACID; CARBOXYMETHYLCELLULOSE; CHITOSAN; MICROSPHERE; POLYANION; POLYCATION; UREA; UREASE;

ARTICLE; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME METABOLISM; HYDROLYSIS; NONHUMAN; PH; WATER TRANSPORT;

EID: 0343340040     PISSN: 10731199     EISSN: None     Source Type: Journal    
DOI: 10.3109/10731190009119788     Document Type: Article

References (23)

1. Ortmanis A, Neufeld RJ, Chang TMS. Study of microencapsulated urease in a continuous feed, stirred tank reactor. Enzyme Microb Technol, 6: 135-139 [1984].
2. Martins MBF, Cruz MEM, Cabral JMS, Kennedy JF. Urease immobilization on an alkylamine derivative of titanium (IV)-porous silica: kinetics and operational stability. J Chem Tech Biotechnol, 39: 201-213 [1987].
3. Elçin YM. Encapsulation of urease enzyme in xanthan-alginate spheres. Biomaterials, 16: 1157-1161 [1995].
4. Chang TMS. A microcapsule artificial kidney: a step toward miniaturization. J Dial Transplant, 4: 23-25 [1975].
5. Lim F, Sun AM. Microencapsulated islets as bioartificial endocrine pancreas. Science, 210: 908-910 [1980].
6. Martinsen A, Skjak-Braek G, Smidsrod O. Alginate as immobilization material: I. correlation between chemical and physical properties of alginate gel beads. Biotechnol Bioeng, 33: 79-89 [1989].
7. Elçin YM. Bacillus sphaericus 2362-alginate microcapsules for mosquito control. Enz Microb Technol, 17: 587-591 [1995].
8. Elçin YM. Control of mosquito larvae by encapsulated pathogene Bacillus thuringiensis var. israelensis. J Microencapsulation, 12: 515-523 [1995].
9. Gilson CD, Thomas AJ. Calcium alginate bead manufacture: with and without immobilised yeast. Drop formation at a two-fluid nozzle. J Chem Tech Biotechnol. 62: 227-232 [1995].
10. Elçin YM, Cokmus C, Sacilik SC. Aluminum-carboxymethylcellulose encapsulation of B. sphaericus 2362 for control of osquito (Culex) larvae. J Econ Entomol, 88: 830-834 [1995].
11. Elçin YM, Dixit V, Gitnick G. Controlled release of endothelial cell growth factor from chitosan-albumin microspheres and fibers for localized angiogenesis: in vitro and in vivo studies. Artif Cells Blood Subs Immob Biotechnol, 24: 257-271 [1996].
12. Weatherburn MW. Enzymic method for urea in urine. Anal Chem, 39: 971-974 [1967].
13. Doumas BT, Bayse DD, Carter RJ. A candidate reference method for determination of total protein in serum. Clin Chem, 27: 1642-1645 [1981].
14. Elçin YM, Akbulut U. Polyester film strips coated with photographic gelatin containing immobilized glucose oxidase hardened by chromium (III) sulphate. Biomaterials, 13: 156-161 [1992].
15. Rosevear M, Kennedy JF, Cabral JMS. Immobilized enzymes and cells. Adam Hilger, Bristol [1987].
16. Goldman R, Goldstein L, Katchalski E. Biomedical aspects of reactions on solid supports. Academic Press, New York [1971].
17. Katchalski E, Silman I, Goldman R. Adv Enzymol, 34: 445 [1971].
18. Kamath N, Melo JS, D'Souza SF. Urease immobilized on polyethyleneimine cotton cloth. Appl Biochem Biotechnol, 19: 251-258 [1988].
19. Elçin YM, Sungur S, Akbulut U. Glucose oxidase immobilized on gelatin by various crosslinkers. Macromol Rep, 30: 137-147 [1993].
20. Elçin YM, Sungur S, Akbulut U. Urease immobilization into poly (acrylamide)-gelatin gels. Bioorg Med Chem Lett, 2: 433-438 [1992].
21. Elçin YM, Saçak M.. Methacrylic acid-acrylamide-g-poly (ethylene terephtalate) fibers for urea hydrolysis. J Chem Tech Biotechnol, 63: 174-180 [1995].
22. Elçin YM, Saçak M.. Acrylamide grafted poly (ethylene terephtalate) fiber activated by glutaraldehyde as support for urease. Appl Biochem Biotechnol, 60: 29-34 [1996].
23. Klibanov AM. Enzyme stabilization by immobilization. Anal Biochem, 93: 1-25 [1979].