Solubilization of delipidated macrophage membrane proteins for analysis by two-dimensional electrophoresis

Electrophoresis

Volumn 21, Issue 3, 2000, Pages 641-644

  Simões Barbosa, Augusto ^a   Santana, Jaime M ^a   Teixeira, Antonio R L ^a  

^a UNIVERSITY OF BRASILIA   (Brazil)

Author keywords

Chemical delipidation; Membrane protein solubilization; Two dimensional electrophoresis

Indexed keywords

ACETONE; ANTIGEN-ANTIBODY REACTIONS; CELL CULTURE; CHLORINE COMPOUNDS; ELECTROPHORESIS; MACROPHAGES; MEMBRANES; PROTEINS; SOAPS (DETERGENTS); SOLUBILITY; SUSPENSIONS (FLUIDS);

AQUEOUS MEDIA; CHEMICAL DELIPIDATION; DELIPIDATION; MACROPHAGE CELL LINE; MEMBRANE PROTEIN SOLUBILISATION; MEMBRANE PROTEINS; MURINE MACROPHAGES; SOLUBILISATION; TWO DIMENSIONAL ELECTROPHORESIS; TWO-DIMENSIONAL GELS;

GELS;

ACETONE; CHLOROFORM; MEMBRANE LIPID; MEMBRANE PROTEIN; METHANOL;

ANIMAL CELL; ARTICLE; CELL MEMBRANE; EXTRACTION; MACROPHAGE; NONHUMAN; PROTEIN ANALYSIS; SOLUBILITY; TWO DIMENSIONAL ELECTROPHORESIS;

EID: 0343340032     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1522-2683(20000201)21:3<641::AID-ELPS641>3.0.CO;2-H     Document Type: Article

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