Large-scale production of mandelate racemase by pseudomonas putida ATCC 12633: Optimization of enzyme induction and development of a stable crude enzyme preparation

Journal of Biotechnology

Volumn 56, Issue 1, 1997, Pages 33-40

  Stecher, Hartmut ^a   Felfer, Ulfried ^a   Faber, Kurt ^a  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

Enzyme induction; Mandelate racemase EC 5.1.2.2 ; Psuedomonas putida ATCC 12633

Indexed keywords

BIOMASS; CATALYST ACTIVITY; GLUCOSE; GROWTH KINETICS;

ENZYME INDUCTION; MANDELATE RACEMASE; PSEUDOMONAS PUTIDA;

ENZYMES;

MANDELIC ACID;

ARTICLE; BIODEGRADATION; BIOMASS; BIOTRANSFORMATION; ENZYME ACTIVITY; ENZYME INDUCTION; PRIORITY JOURNAL; PSEUDOMONAS PUTIDA;

EID: 0343329771     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(97)00076-X     Document Type: Article

References (12)

1. Adams, E., 1976. Catalytic aspects of enzymatic racemization. Adv. Enzymol. Relat. Areas Mol. Biol. 44, 69-138.
2. Fee, J.A., Hegeman, G.D., Kenyon, G.L., 1974. Mandelate racemase from Pseudomonas putida. Subunit composition and absolute divalent metal ion requirement. Biochemistry 12, 2528-2532.
3. Gunsalus, C.F., Stanier, R.Y., Gunsalus, I.C., 1953. The enzymatic conversion of mandelic acid to benzoic acid. III. Fractionation and properties of the soluble enzymes. J. Bacteriol. 66, 548-553.
4. Hegeman, G.D., 1966. Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. J. Bacteriol. 91, 1140-1154.
5. Hegeman, G.D., Rosenberg, E.Y., Kenyon, G.L., 1970. Mandelic acid racemase from Pseudomonas putida. Purification and properties of the enzyme. Biochemistry 9, 4029-4035.
6. Kenyon, G.L., Hegeman, G.D., 1979. Mandelate racemase. Adv. Enzymol. Relat. Areas Mol. Biol. 50, 325-360.
7. Kenyon, G.L., Gerlt, J.A., Petzko, G.A., Kozarich, J.W., 1995. Mandelate racemase: structure-function studies of a pseudosymmetric enzyme. Acc. Chem. Res. 28, 178-186.
8. Landro, J.A., Kenyon, G.L., Kozarich, J.W., 1992. Mechanism-based inactivation of mandelate racemase by propargylglycolate. Bioorg. Med. Chem. Lett. 2, 1411-1418.
9. Li, R., Powers, V.M., Kozarich, J.W., Kenyon, G.L., 1995. Racemization of vinylglycolate catalyzed by mandelate racemase. J. Org. Chem. 60, 3347-3351.
10. Petsko, G.A., Kenyon, G.L., Gerlt, J.A., Ringe, D., Kozarich, J.W., 1993. On the origin of enzymatic species. Trends Biochem. Sci. 18, 372-376.
11. Stanier, R.Y., Palleroni, N.J., Doudoroff, M., 1966. The aerobic pseudomonads: a taxonomic study. J. Gen. Microbiol. 43, 159-271.
12. Stecher, H., Faber, K., 1997. Biocatalytic de-racemization techniques: dynamic resolutions and stereoinversions. Synthesis, 1-16.