Protein retention in ion-exchange chromatography: Effect of net charge and charge distribution

Journal of Chromatography A

Volumn 877, Issue 1-2, 2000, Pages 13-24

  Hallgren, Elisabeth ^a   Kálmán, Franka ^b   Farnan, Dell ^b   Horváth, Csaba ^b   Ståhlberg, Jan ^a  

^a STOCKHOLM UNIVERSITY   (Sweden)

^b Yale University   (United States)

Author keywords

Charge distribution; Enzymes; Net charge; Nuclease; Protein retention; Retention models; Staphylococcal nuclease

Indexed keywords

ISOENZYME; MUTANT PROTEIN; NUCLEASE; SODIUM CHLORIDE;

ARTICLE; CONTROLLED STUDY; ELECTROCHEMISTRY; ENZYME BINDING; EXPERIMENTAL MODEL; ION EXCHANGE CHROMATOGRAPHY; NONHUMAN; PH; POINT MUTATION; PRIORITY JOURNAL; REACTION ANALYSIS; STRUCTURE ACTIVITY RELATION; SURFACE CHARGE; VALIDATION PROCESS;

CHROMATOGRAPHY, ION EXCHANGE; HYDROGEN-ION CONCENTRATION; MICROCOCCAL NUCLEASE; POINT MUTATION;

EID: 0343293935     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0021-9673(00)00190-4     Document Type: Article

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