Heterotrimeric G-protein β-subunit is localized in the plasma membrane and nuclei of tobacco leaves

Plant Molecular Biology

Volumn 42, Issue 6, 2000, Pages 915-922

  Peškan, Tatjana ^a   Oelmüller, Ralf ^a  

^a Lehrst fur Pflanzenphysiologie   (Germany)

Author keywords

Heterotrimeric G proteins; Subcellular localization; Tobacco

Indexed keywords

CELL FRACTIONATION; CELL MEMBRANE; CELL NUCLEUS; GUANINE NUCLEOTIDE BINDING PROTEIN; PLANT DEVELOPMENT; PLANT LEAF; PROTEIN LOCALIZATION; PROTEIN SUBUNIT; TOBACCO;

ANTIBODIES; BLOTTING, WESTERN; CELL FRACTIONATION; CELL MEMBRANE; CELL NUCLEUS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HETEROTRIMERIC GTP-BINDING PROTEINS; MICROSOMES; PLANT LEAVES; PLANTS, TOXIC; TOBACCO;

NICOTIANA TABACUM;

EID: 0343238146     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1006477631166     Document Type: Article

References (37)

1. Adamska, I., Kloppstech, K. and Ohad, I. 1993. Early light-inducible protein in pea is stable during light stress, but is degraded during recovery at low light intensities. J. Biol. Chem. 268: 5438-5444.
2. Bartlett, S.G., Grossmann, A.R. and Chua, N.-H. 1982. In vitro synthesis and uptake of cytoplasmically-synthesized chloroplast proteins. In: M. Edelman, R.B. Hallick and N.-H. Chua (Eds.), Methods in Chloroplast Molecular Biology, Vol. 86, Elsevier Biomedical Press, Amsterdam/New York/Oxford, pp. 1081-1091.
3. Beffa, R., Szell, M., Meuwly, P., Pay, A., Vögeli-Lange, R., Metraux, J.P., Neuhaus, G., Meins, F. and Nagy, F. 1995. Cholera toxin elevates pathogen resistance and induces pathogenesis-related gene expression in tobacco. EMBO J. 14: 5753-5761.
4. Birnbaumer, L. 1992. Receptor-to-effector signaling through G proteins: Roles for beta-gamma dimers as well as alpha subunits. Cell 71: 1069-1072.
5. Bowler, C. and Chua, N.-H. 1994. Emerging themes of plant signal transduction. Plant Cell 6: 1529-1541.
6. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
7. Briskin, D.P., Leonard, R.T. and Hodges, T.K. 1987. Isolation of the plasma membrane: Membrane markers and general principles. Meth. Enzymol. 148: 542-568.
8. Clark, G.B., Memon, A.R., Tong, C.-G., Thompson, G.A. Jr. and Roux, S.J. 1993. Phytochrome regulates GTP-binding protein activity in the envelope of pea nuclei. Plant J. 4: 399-402.
9. Drøbak, B.K., Watkins, P.A.C., Bunney, T.D., Dove, S.K., Shaw, P.J., White, I.R. and Millner, RA. 1995. Association of multiple GTP-binding proteins with the plant cytoskeleton and nuclear matrix. Biochem. Biophys. Res. Commun. 210: 7-13.
10. Fairley-Gremont, K. and Assmann, S.M. 1991. Evidence for G-protein regulation of inward potassium ion channel current in guard cells of faba bean. Plant Cell 3: 1037-1044.
11. Håkansson, G., van der Mark, F., Bonnett, H.T. and Glimelius, K. 1988. Variant mitochondrial protein and DNA patterns associated with cytoplasmic male-sterile lines of Nicotiana. Theor. Appl. Genet. 76: 431-437.
12. Henriksson, D. and Eriksson, I. 1988. Serine hydroxymethyl-transferase from spinach leaf mitochondria. Purification and characterization. Physiol. Plant. 74: 602-606.
13. Higgins, J.B. and Casey, P.J. 1994. In vitro processing of recombinant G-protein subunits. J. Biol. Chem. 269: 9067-9073.
14. Hodges, T.K. and Mills, D. 1986. Isolation of the plasma membrane. Meth. Enzymol. 118: 41-54.
15. Hooley, R. 1998. Plant hormone perception and action: A role for G-protein signal transduction? Phil Trans. R. Soc. Lond. B Biol. Sci. 353: 1425-1430.
16. Kaufman, L.S. 1993. Transduction of blue-light signals. Plant Physiol. 102: 333-337.
17. Koop, H.-U., Steinmüller, K., Wagner, H., Rössler, C., Eibl, C. and Sacher, L. 1996. Integration of foreign sequences into the tobacco plastome via polyethylene glycol-mediated protoplast transformation. Planta 199: 193-201.
18. Kusnetsov, V.V. and Oelmüller, R. 1996. Isolation and characterization of cDNAs encoding the subunit beta of heterotrimeric G proteins from N. tabacum (accession No. X98161). Plant Physiol. 111: 948.
19. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
20. + current in mesophyll cells of Vicia faba L. Proc. Natl. Acad. Sci. USA 90: 262-266.
21. Ma, H. 1996. GTP binding proteins in plants: New members of an old family. Plant Mol. Biol. 26: 1611-1636.
22. Matsuda, T., Hashimoto, Y., Ueda, H., Asano, T., Matsuura, Y. Doi, T., Takao, T., Shimonishi, Y and Fukuda, Y. 1998. Specific isoprenyl group linked to transducin gamma subunit is a determinant of its unique signaling properties among G-proteins. Biochemistry 37: 9843-9850.
23. Milligan, G. and Grassie, M. A. 1997. How do G-proteins stay at the plasma membrane? Essays Biochem. 32: 49-60.
24. Neer, E.J. 1995. Heterotrimeric G proteins: Organizers of transmembrane signals. Cell 80: 249-257.
25. Neuhaus, G., Bowler, C., Kern, R. and Chua, N.-H. 1993. Calcium/calmodulin-dependent and independent phytochrome signal transduction pathways. Cell 73: 937-952.
26. Pennington, S.R. 1995. GTP-binding proteins: Heterotrimeric G proteins. Protein Profile 1 (3), Academic Press, New York.
27. Plakidou-Dymock, S., Dymock, D. and Hooley, R. 1998. A higher plant seven-transmembrane receptor that influences sensitivity to cytokinins. Curr. Biol. 8 (6): 315-324.
28. Romero, L. and Lam, E. 1993. Guanine nucleotide binding protein involvement in early steps of phytochrome-regulated gene expression. Proc. Natl. Acad. Sci. USA 90: 1465-1469.
29. Sakamoto, K. and Nagatani, A. 1996. Nuclear localization activity of phytochrome B. Plant J. 10: 859-868.
30. Schürmann, A., Rosenthal, W., Schultz, G. and Joost, H.G. 1992. Characterization of GTP-binding proteins in Golgi-associated membrane vesicles from rat adipocytes. Biochem J. 283: 795-801.
31. Simon, M.I., Strathmann, P. and Gautam, N. 1991. Diversity of G proteins in signal transduction. Science 252: 802-808.
32. Takei, Y., Takahashi, K., Kanaho, Y. and Katada, T. 1994. Possible involvement of a pertussis toxin-sensitive GTP-binding protein in protein transport into nuclei isolated from rat liver. J. Biochem. 115:578-583.
33. Tittgen, J., Hermans, J., Steppuhn, J., Janson, Th., Jansson, Ch., Andersson, B., Nechushtai, R., Nelson, N. and Herrmann, R.G. 1986. Isolation of cDNA clones for fourteen nuclear-encoded thylakoid proteins. Mol. Gen. Genet. 204: 258-265.
34. Warpeha, K.M.F., Hamm, H.E., Rasenick, M.M. and Kaufman, L.S. 1991. A blue light activated GTP binding protein in the plasma membrane of etiolated pea. Proc. Natl. Acad. Sci. USA 88: 8925-8929.
35. Weiss, C.A., Garnaat, C.W., Mukai, K., Hu, Y and Ma, H. 1994. Isolation of cDNAs encoding guanine nucleotide-binding protein beta-subunit homologues from maize (ZGB1) and Arabidopsis (AGB1). Proc. Natl. Acad. Sci. USA 91: 9554-9558.
36. Weiss, C.A., White, E., Huang, H. and Ma, H. 1997. The G-protein a subunit (Gpα 1) is associated with the ER and the plasma membrane in meristematic cells of Arabidopsis and cauliflower. FEBS Lett. 407: 361-367.
37. Yasuda, H., Lindorfer, M.A., Woodfork, K.A., Fletcher, J.E. and Garrison, J.C. 1996. Role of the prenyl group on the G-protein a subunit in coupling trimeric G-proteins to A1 adenosine receptors. J. Biol. Chem. 271: 18588-18595.