2,4-Dichlorophenoxyacetic acid disrupts the cytoskeleton and disorganizes the Golgi apparatus of cultured neurons

Toxicological Sciences

Volumn 56, Issue 1, 2000, Pages 133-140

  Rosso, Silvana B ^a,c   Cáceres, Alfredo O ^b   De Duffard, Ana Maria Evangelista ^a   Duffard, Ricardo O ^a   Quiroga, Santiago ^c  

^a UNIVERSIDAD NACIONAL DE ROSARIO   (Argentina)

^b INSTITUTO DE INVESTIGACIÓN MÉDICA MERCEDES Y MARTÍN FERREYRA   (Argentina)

^c UNIVERSIDAD NACIONAL DE CÓRDOBA   (Argentina)

Author keywords

2,4 D; Developing neurons; Gangliosides; Golgi apparatus; Microtubules; Neurotoxicity

Indexed keywords

2,4 DICHLOROPHENOXYACETIC ACID; GANGLIOSIDE; HERBICIDE; TUBULIN;

ANIMAL CELL; ANIMAL MODEL; ARTICLE; CELL VIABILITY; CONTROLLED STUDY; CYTOPATHOLOGY; CYTOTOXICITY; DOSE RESPONSE; GOLGI COMPLEX; GRANULE CELL; LIPID ANALYSIS; MICROTUBULE ASSEMBLY; NERVE CELL GROWTH; NEUROTOXICITY; NEWBORN; NONHUMAN; PROTEIN POLYMERIZATION; RAT;

ANIMALIA;

EID: 0343167942     PISSN: 10966080     EISSN: None     Source Type: Journal    
DOI: 10.1093/toxsci/56.1.133     Document Type: Article

References (48)

1. Abe, T., Kakyo, M., Sakagami, H., Tokui, T., Nishio, T., Tanemoto, M., Nomura, H., Hebert, S.C., Matsuno, S., Kondo, H., and Yawo, H. (1998). Molecular characterization and tissue distribution of a new organic anion transporter subtype (oatp3) that transports thyroid hormones and taurocholate and comparison with oatp2. J. Biol. Chem. 273, 22395-22401.
2. Ando, S. (1983). Gangliosides in the nervous system. Neurochem. Int. 5, 507-537.
3. 2+ homeostasis alterations induced by 2.4-dichlorophenoxyacetic butyl ester and 2,4-dichlorophenoxyacetic acid on avian skeletal muscle. Biochem. Pharmacol. 40, 2441-2448.
4. Bergesse, J. R., and Bulegno, H. F. (1995). 2,4-Dichlorophenoxyacetic acid influx is mediated by an active transport system in Chinese hamster ovary cells. Toxicol. Lett. 81, 167-173.
5. Bernard, P., Toyoshima, E., Eccles, C., Mayer, R., Johnson, K., and Max, S. (1985). 2,4-Dichlorophenoxyacetic acid (2,4-D) reduces acetylcholinesterase activity in rat muscle. Exp. Neurol. 87, 544-556.
6. 14C on thin layers. Anal. Biochem. 89, 247-256.
7. Borisy, G. G., Marcum, J. M., Olmsted, J. B., Murphy, D. B., and Johnson, K. A. (1975). Purification of tubulin and associated high molecular weight proteins from porcine brain and characterization of microtubule assembly in vitro. Ann. N Y Acad. Sci. 253, 107-132.
8. Brusco, A., Saavedra, J. P., García, G., Tagliaferro, P., Evangelista de Duffard, A. M., and Duffard, R. O. (1997). 2,4-Dichlorophenoxyacetic acid through lactation induces astrogliosis in rat brain. Mol. Chem. Neuropathol. 30, 175-185.
9. Busciglio, J., Lorenzo, A., and Yankner, B. A. (1992). Methodological variables in the assesment of β amyloid neurotoxicity. Neurobiol. Aging 13, 609-612.
10. Cáceres, A., Mautino, J., and Kosik, K. (1992). Suppression of MAP2 in cultured cerebellar macroneurons inhibits minor neurite formation. Neuron 9, 607-618.
11. DiTella, M., Feiguin, F., Morfini, G., and Cáceres, A. (1994). Microfilament-associated growth cone component depends upon Tau for its intracellular localization. Cell Motil. Cytoskeleton. 29, 117-130.
12. Duffard, R., Garcia, G., Rosso, S., Bortolozzi, A., Madariaga, M., di Paolo, O., and Evangelista de Duffard, A. M. (1996). Central nervous system myelin deficit in rats exposed to 2,4-dichlorophenoxyacetic acid throughout lactation. Neurotoxicol. Teratol. 18, 691-696.
13. Drubin, D., Feinstein, S., Shooter, E., and Kirschner, M. (1985). Nerve growth factor-induced neurite outgrowth in PC12 cells involves the coordinate induction of microtubule assembly and assembly-promoting factors. J. Cell Biol. 101, 1799-1807.
14. Evangelista, A. M., Bortolozzi, A., and Duffard, R. O. (1995). Altered behavioral responses in 2,4-dichlorophenoxyacetic acid-treated and amphetamine-challenged rats. Neurotoxicology 16, 479-488.
15. Feiguin, F., Ferreira, A., Kosik, K., and Caceres, A. (1994). Kinesin-mediated organelle translocation revealed by specific cellular manipulations. J. Cell Biol. 127, 1021-1039.
16. Feldman, R. J., and Maibach, H. I. (1974). Percutaneous penetration of some pesticides and herbicides in man. Toxicol. Appl. Pharmacol. 28, 126-132.
17. Gallo, V., Kingsbury, A., Balazs, R., and Jorgensen, O. (1987). The role of depolarization in the survival and differentiation of cerebellar granule cells in culture. J. Neurosci. 7, 2203-2213.
18. Giraudo, C. G., Rosales Fritz. V. M., and Maccioni, H. J. (1999). GA2/GM2/ GD2 synthase localizes to the trans-Golgi network of CHO-KI cells. Biochem. J. 342, 633-640.
19. Goold, R. G., Owen, R., and Gordon-Weeks, P. R. (1999). Glycogen synthase kinase 3β phosphorylation of microtubule-associated protein 1β;regulates the stability of microtubules in growth cones J. Cell Sci. 112, 3373-3384.
20. Grover, R., Franklin, C. A., Muir, N. I., Cessna, A. J., and Riedel, D. (1986). Dermal exposure and urinary metabolite excretion in farmers repeatedly exposed to 2,4-D amine. Toxicol. Lett. 33, 73-83.
21. Karecla, P., and Kreis, T. (1992). Interaction of membranes of the Golgi complex with microtubules in vitro. Eur. J. Cell Biol. 57, 139-146.
22. Kivatinitz, S., Grabois, V., and Quiroga, S. (1995). High-density lipoprotein inhibits UDP-N-acetylgalactosamine:GM3, N-acetylgalactosaminyltransferase and differentiation of cultured cerebral cells: Comparison with a formerly described inhibitor of this enzyme. J. Neurochem. 65, 775-781.
23. Kivatinitz, S., Pelsman, M., Alonso, A., Bagutolli, L., and Quiroga, S. (1997). High-density lipoprotein aggregated by oxidation induces degeneration of neuronal cells. J. Neurochem. 69, 2102-2114.
24. Kodavanti, P. R., Shin, D. S., Tilson, H. A., and Harry, G. J. (1993). Comparative effects of two polychlorinated biphenyl congeners on calcium homeostasis in rat cerebellar granule cells. Toxicol. Appl. Pharmacol. 123, 97-106.
25. Kreis, T. (1990). Role of microtubules in the organisation of the Golgi apparatus. Cell Motil. Cytoskeleton 15, 67-70.
26. Labarca, C., and Paigen, K. (1980). A simple, rapid, and sensitive DNA assay procedure. Anal. Biochem. 102, 344-352.
27. Lucas, F. R., Goold, R. G., Gordon-Weeks, P. R., and Salinas, P. C. (1998). Inhibition of GSK-3beta leading to the loss of phosphorylated MAP-1B is an early event in axonal remodeling induced by WNT-7a or lithium. J. Cell Sci. 111, 1351-1361.
28. Maccioni, H. J., Daniotti, J. L. and Martina, J. A. (1999). Organization of ganglioside synthesis in the Golgi apparatus. Biochim. Biophys. Acta 1437, 101-118.
29. Mattsson, J., Charles, J., Yano, B., Cunny, L., Wilson, R., and Bus, J. (1997). Single-dose and chronic dietary neurotoxicity screening studies on 2.4-dichlorophenoxyacetic acid in rats. Fundam. Appl. Toxicol. 40, 111-119.
30. Mohammad, F., and St. Omer, V. (1986). Behavioral and developmental effects in rats following in utero exposure to 2,4-D/2,4,5-1 mixture. Neurobehav. Toxicol. Teratol 8, 551-560.
31. Mori de Moro, G. B., Duffard, R. O., and Evangelista de Duffard, A. M. (1985). Chick brain hypomyelination produced by 2,4-dichlorophenoxyace-tic butyl ester treatment. Neurotoxicology 6, 133-137.
32. Nakata, T., and Hirokawa, N. (1987). Cytoskeletal reorganization of human platelets after stimulation revealed by the quick-freeze deep-etch technique. J. Cell. Biol. 105, 1771-1780.
33. O'Brien, E. T., Salmon, E. D. and Erickson, H. P. (1997). How calcium causes microtubule depolymerization. Cell Motil. Cystoskeleton 36, 125-135.
34. Ogiso, M., Ohta, M., Harada, Y., and Hirano, S. (1992). Effect of colchicine on ganglioside composition of rat primary culture neurons. Dev. Neurosci. 14, 53-60.
35. Oliveira, G., and Palermo-Neto, J. (1993). Effects of 2,4-dichlorophenoxyacetic acid (2,4-D) on open-field behaviour and neurochemical parameters of rats. Pharmacol. Toxicol. 73, 79-85.
36. Pagano, R. E., Martin, O. C., Kang, H. C., and Haugland, R. P. (1991). A novel fluorescent ceramide analogue for studying membrane traffic in animal cells: Accumulation at the Golgi apparatus results in altered spectral properties of the sphingolipid precursor. J. Cell Biol. 113, 1267-1279.
37. Paglini, G., Kunda, P., Quiroga, S., Kosik, K., and Cácercs, A. (1998). Suppression of radixin and moesin alters growth cone morphology, motility, and process formation in primary cultured neurons. J. Cell Biol. 143, 443-455.
38. Palmeira, C. M., Moreno, A. J., and Madeira, V. M. (1995). Effects of paraquat, dinoseb, and 2,4-D on intracellular calcium and on vasopressin-induced calcium mobilization in isolated hepatocytes. Arch. Toxicol. 69, 460-466.
39. Rosso, S., Di Paolo, O., Evangelista de Duffard, A. M., and Duffard, R. (1997). Effects of 2,4-dichlorophenoxyacetic acid on central nervous system of developmental rats. Associated changes in ganglioside pattern. Brain Res. 769, 163-167.
40. Rosso, S., García, G., Madariaga, M., Evangelista de Duffard, A. M., and Duffard, R. (2000). 2,4-Dichlorophenoxyacetic acid in developing rats alters behaviour, myelination, and regions brain gangliosides pattern. Neurotoxicology 21, 155-164.
41. 2+ and inositol phosphate levels in cultured cerebellar granule neurons. J. Neurochem. 61, 648-657.
42. Scheel, J., Matteoni, R., Ludwig, T., Hoflack, B., and Kreis, T. (1990). Microtubule depolymerization inhibits transport of cathepsin D from the Golgi apparatus to lysosomes. J. Cell Sci. 96, 711-720.
43. Sheikh, K. A., Sun, J., Liu, Y., Kawai, H., Crawford, T. O., Proia, R. L., Griffin, J. W., and Schnaar, R. L. (1999). Mice lacking complex gangliosides develop Wallerian degeneration and myelination defects. Proc. Natl. Acad. Sci. U S A 96, 7532-7537.
44. van Echten, G., and Sandhoff, K. (1989). Modulation of ganglioside biosynthesis in primary cultured neurons. J. Neurochem. 52, 207-214.
45. Villalobos, A. R., Dunnick, C. A., and Pritchard, J. B. (1996). Mechanism mediating basolateral transport of 2,4-dichlorophenoxyacetic acid in rat kidney. J. Pharmacol. Exp. Ther. 278, 582-589.
46. Weingarten, M. D., Lockwood, A. H., Hwo, S. Y., and Kirschner, M. W. (1975). A protein factor essential for microtubule assembly. Proc. Natl. Acad. Sci. U S A 72, 1858-1862.
47. 18 cartridges during the isolation of gangliosides. J. Neurochem. 35, 266-269.
48. Zhao, Y., Li, W., and Chou, I. N. (1987). Cytoskcletal perturbation induced by herbicides, 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T). J. Toxicol. Environ. Health 20, 11-26.