Antisense expression of protein kinase Cα improved sensitivity to anticancer drugs in human lung cancer LTEPa-2 cells

Acta Pharmacologica Sinica

Volumn 19, Issue 3, 1998, Pages 265-268

  Wang, Xiang Yang ^a   Liu, Hui Tu ^a  

^a BEIJING NORMAL UNIVERSITY   (China)

Author keywords

Antisense RNA; Cultured tumor cells; Doxorubicin; Harringtonines; Lung neoplasms; MDR genes; Multiple drag resistance; Northern blotting; Protein kinase C; Western blotting

Indexed keywords

ANTINEOPLASTIC AGENT; BLEOMYCIN; CARBOPLATIN; COMPLEMENTARY RNA; DOXORUBICIN; PROTEIN KINASE; VINCRISTINE;

ANTINEOPLASTIC ACTIVITY; ARTICLE; DRUG RESPONSE; GENE EXPRESSION; LUNG CANCER; MULTIDRUG RESISTANCE;

ADENOCARCINOMA; ANTINEOPLASTIC AGENTS; DRUG RESISTANCE, MULTIPLE; GENE EXPRESSION REGULATION; GENES, MDR; HUMANS; ISOENZYMES; LUNG NEOPLASMS; PROTEIN KINASE C; PROTEIN KINASE C-ALPHA; RNA, ANTISENSE; TUMOR CELLS, CULTURED;

EID: 0343137366     PISSN: 02539756     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Hofmann J, Fiebig HH, Winterhalter BR, Berger DP, Grunicke H. Enhancement of the antiproliferative activity of cis-diamminedichloroplatinum (II) by quercetin. Int J Cancer 1990; 45: 536-9.
2. Basu A. The potential of protein kinase C as a target for anticancer treatment. Pharmacol Ther 1993; 59: 257-80.
3. Housey GM, Johnson MD, Hsiao WLW, O'Brian CA, Murphy JP, Kirschmeier P, et al. Overproduction of protein kinase C causes disordered growth control in rat fibroblasts. Cell 1988; 52: 343-54.
4. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987; 162: 156-9.
5. Christen RD, Isonishi S, Jones JA, Jekunen AP, Horn DK, Kroning R, et al. Signaling and drug sensitivity. Cancer Metastasis Rev 1994; 13: 175-89.
6. Nooter K, Herweijer H. Multidrug resistance (mdr) genes in human cancer. Br J Cancer 1991; 63: 663-9.
7. Ueda K, Cardarelli C, Gottesman MM, Pastan I. Expression of a full-length cDNA for the human "MDR1" gene confers resistance to colchicine, doxorubicin and vinblastine. Proc Natl Acad Sci USA 1987; 84: 3004-8.
8. Fine RL, Patel J, Chabner BA. Phorbol esters induce multidrug resistance in human breast cancer cells. Proc Natl Acad Sci USA 1988; 85: 582-6.
9. O'Brian CA, Ward NE, Liskamp RM, de Bont DB, Earnest LE, van Boom JH, et al. A novel N-myristylated synthetic octapeptide inhibits protein kinase C activity and partially reverses murine fibrosarcoma cell resistance to adriamycin. Invest New Drugs 1991; 9: 169-79.
10. Chambers TC, McAvoy EM, Jacobs JW, Eilon G. Protein kinase C phosphorylates P-glycoprotein in multidrug resistant human KB carcinoma cells. J Biol Chem 1990; 265: 7679-86.
11. Volm M, Pommerenke E W. Associated expression of protein kinase C with resistance to doxorubicin in human lung cancer. Anticancer Res 1995; 15: 463-6.
12. Yu G, Ahmad S, Aquino A, Fairchild CR, Trepel JB, Ohno S, et al. Transfection with protein kinase Cα confers increased multidrug resistance to MCF-7 cells over-expressing P-glycoprotein. Cancer Commun 1991; 3: 181-9.
13. Ahmad S, Glazer RI. Expression of the antisense cDNA for protein kinase Cα attenuates resistance in doxorubicin-resistant MCF-7 breast carcinoma cells. Mol Pharmacol 1993; 43: 858-62.
14. Teeter LD, Eckersberg T, Tsai Y, Kuo MT. Analysis of the Chinese hamster P-glycoprotein/multidrug resistance gene pgp 1 reveals that the AP-1 site is essential for full promoter activity. Cell Growth Differ 1991; 2: 429-37.