Determination of L-carnitine by flow injection analysis with NADH fluorescence detection

Analytical Biochemistry

Volumn 281, Issue 2, 2000, Pages 176-181

  Manjon A ^a   Obon J M ^a   Iborra, J L ^a  

^a UNIVERSITY OF MURCIA   (Spain)

Author keywords

Flow injection analysis; Immobilized L carnitine dehydrogenase; L carnitine; NADH fluorescence

Indexed keywords

ENZYMES;

DETECTION LIMITS; FLOW INJECTION ANALYSIS; FLOW INJECTION ANALYSIS METHODS; FLUORESCENCE DETECTION; HIGH SENSITIVITY; L-CARNITINE; NADH FLUORESCENCE; PHARMACEUTICAL PREPARATIONS;

FLUORESCENCE;

CARNITINE; DRUG; IMMOBILIZED ENZYME; OXIDOREDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE;

ACCURACY; ARTICLE; DRUG ANALYSIS; ENZYME IMMOBILIZATION; FLOW INJECTION ANALYSIS; FLUORESCENCE; INTERMETHOD COMPARISON; PRIORITY JOURNAL;

EID: 0343133884     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4573     Document Type: Article

References (20)

1. Carter, A. L., Abney, T. O., and Lapp, D. F. (1995) Biosynthesis and metabolism of carnitine. J. Child Neurol. 10, S3-S7.
2. Marzo, A., and Curti, S. (1997) L-Carnitine moiety assay: An up-to-date reappraisal covering the commonest methods for various applications. J. Chromatogr. B 72, 1-20.
3. Kamimori, H., Hamashida, Y., and Konishi, M. (1994) Determination of carnitine and saturated acyl group carnitines in human urine by high-performance liquid chromatography with fluorescence detection. Anal. Biochem. 218, 417-424.
4. Minkler, P. E., Brass, E. P., Hiatt, W. R., Ingalls, S. T., and Hoppel, C. L. (1995) Quantification of carnitine, acetylcarnitine, and total carnitine in tissues by high-performance liquid chromatography: The effect of exercise on carnitine homeostasis in man. Anal. Biochem. 231, 315-322.
5. Hirota, T., Minato, K., Ishii, K., Nishimura, N., and Sato, T. (1994) High-performance liquid chromatographic determination of the enantiomers of carnitine and acetylcarnitine on a chiral stationary phase. J. Chromatogr. A 673, 37-43.
6. Takahashi, M., Terashima, K., Nishijima, M., and Katama, K. (1996) Separation of carnitine enantiomers as the 9-anthroylnitrile derivatives and HPLC analysis on an ovomucoid-conjugated column. J. Pharm. Biomed. Anal. 14, 1579-1584.
7. Vogt, C., Georgi, A., and Werner, G. (1995) Enantiomeric separation of D/L carnitine using HPLC and CZE after derivatization. Chromatographia 40, 287-295.
8. Wieland, O. H., Deufel, T., and Paetzke-Brunner, J. (1985) in Methods of Enzymatic Analysis (Bergmeyer, H. U., Ed.), Vol. VIII, pp. 481-488, Verlag Chemie, Weinheim.
9. Marzo, A., Cardace, G., and Arrigoni Martelli, E. (1992) Enzymes in stereoselective pharmacokinetics of endogenous substances. Chirality 4, 247-251.
10. Ogbomo, I., Kittsteiner-Eberle, R., and Englbrecht, U. (1990) Flow-injection systems for the determination of oxidoreductase substrates: Application in food quality control and process monitoring. Anal. Chim. Acta 249, 137-143.
11. Keay, P. J., and Wang, Y. (1997) Applications of flow-injection analysis to analytical biotechnology. Trends Biotechnol. 17, 76-81.
12. Van der Pol, J. J., de Gooijer, C. D., and Biselli, M. (1996) Automatization of selective assays for on-line bioprocess monitoring by FIA. Trends Biotechnol. 14, 471-477.
13. Ferreira, I. M. P. L. V. O., Macedo, M. N., and Ferreira, M. A. (1997) Enzymatic flow injection determination of free L-carnitine in infant formulae. Analyst 122, 1539-1541.
14. Schöpp, W., and Schäfer, A. (1985) Quantitative bestimmung von L-carnitin mit hilfe van carnitindehydrogenase aus Pseudomonas putida. Fresenius' Z. Anal. Chem. 320, 285-289.
15. Takahashi, M., Ueda, S., Misaki, H., Sugiyama, N., Matsumoto, K., Matsuo, N., and Murao, S. (1994) Carnitine determination by an enzymatic cycling method with carnitine dehydrogenase. Clin. Chem. 40, 817-821.
16. Matsumoto, K., Yamada, Y., Takahashi, M., Todoroki, T., Mizoguchi, K., Misaki, H., and Yuki, H. (1990) Fluorometric determination of carnitine in serum with immobilized carnitine dehydrogenase and diaphorase. Clin. Chem. 36, 2072-2076.
17. Schügerl, K., Hitzmann, B., Jurgens, H., and Kullick, T. (1996) Challenges in integrating biosensors and FIA for on-line monitoring and control. Trends Biotechnol. 14, 21-30.
18. Comtat, M., Galy, M., Goulas, P., and Souppe, J. (1988) Amperometric bienzyme electrode for L-carnitine. Anal. Chim. Acta 208, 295-300.
19. Hanschmann, H., Ehricht, R., and Kleber, H-P. (1996) Purification and properties of L-carnitine dehydrogenase from Agrobacterium sp. Biochim. Biophys. Acta 1290, 177-183.
20. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.