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Volumn 35, Issue 1, 1997, Pages 1-10

'In gel' cleavage with cyanogen bromide for protein internal sequencing

Author keywords

'In gel' CNBr cleavage; Edman degradation; Electroblotting; Gel electrophoresis; Protein sequence analysis

Indexed keywords

CYANOGEN BROMIDE;

EID: 0343052541     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(97)00018-3     Document Type: Article
Times cited : (9)

References (8)
  • 1
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    • Gross E, Witkop B. Nonenzymatic fragmentation of proteins was first successfully applied to bovine pancreatic ribonuclease. J Biol Chem 1962;237:1856-960.
    • (1962) J Biol Chem , vol.237 , pp. 1856-1960
    • Gross, E.1    Witkop, B.2
  • 2
    • 13144267947 scopus 로고
    • Hirs CHW, editor. New York and London, Academic Press
    • Gross E. In: Hirs CHW, editor. Methods in Enzymology Vol XI. New York and London, Academic Press, 1967:238-55.
    • (1967) Methods in Enzymology , vol.11 , pp. 238-255
    • Gross, E.1
  • 3
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    • Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications
    • Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979;76:4350-4.
    • (1979) Proc Natl Acad Sci USA , vol.76 , pp. 4350-4354
    • Towbin, H.1    Staehelin, T.2    Gordon, J.3
  • 4
    • 0023430927 scopus 로고
    • Internal aminoacid sequence analysis of proteins separated by one or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose
    • Aebersold RH, Leavitt J, Saavedra RA, Hood LE, Kent SBH. Internal aminoacid sequence analysis of proteins separated by one or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose. Proc Natl Acad Sci USA 1987;84:6970-4.
    • (1987) Proc Natl Acad Sci USA , vol.84 , pp. 6970-6974
    • Aebersold, R.H.1    Leavitt, J.2    Saavedra, R.A.3    Hood, L.E.4    Kent, S.B.H.5
  • 5
    • 0026694947 scopus 로고
    • In-gel digestion of proteins for internal sequence analysis after one or two-dimensional gel electrophoresis
    • Rosenfeld J, Capdevielle J, Guillemot JC, Ferrara P. In-gel digestion of proteins for internal sequence analysis after one or two-dimensional gel electrophoresis. Anal Biochem 1992;203:173-9.
    • (1992) Anal Biochem , vol.203 , pp. 173-179
    • Rosenfeld, J.1    Capdevielle, J.2    Guillemot, J.C.3    Ferrara, P.4
  • 6
    • 0028983813 scopus 로고
    • Improvement of an 'in-gel' digestion procedure for the micropeparation of internal protein fragments for amino acid sequencing
    • Hellman U, Wernstedt C, Góñez J, Heldin CH. Improvement of an 'in-gel' digestion procedure for the micropeparation of internal protein fragments for amino acid sequencing. Anal Biochem 1995;224:451-5.
    • (1995) Anal Biochem , vol.224 , pp. 451-455
    • Hellman, U.1    Wernstedt, C.2    Góñez, J.3    Heldin, C.H.4
  • 7
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    • Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDs
    • Schägger H, von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDs. Anal Biochem 1987;166:368-79.
    • (1987) Anal Biochem , vol.166 , pp. 368-379
    • Schägger, H.1    Von Jagow, G.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.