Crystallization and preliminary diffraction analysis of Ca2+- calmodulin-drug and apocalmodulin-drug complexes

Proteins: Structure, Function and Genetics

Volumn 28, Issue 1, 1997, Pages 131-134

  Vertessy, Beata G ^a   Böcskei, Zsolt ^b,c   Harmath, Veronika ^b   Náray Szabó, Gábor ^b   Ovádi, Judit ^a  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

^b EÖTVÖS LORÁND UNIVERSITY   (Hungary)

^c Chinoin Pharmaceutical and Chemical Works Ltd   (Hungary)

Author keywords

apocalmodulin; bisindol alkaloids; calmodulin antagonists; circular dichroism spectroscopy; fendiline analogues; protein crystallization; X ray diffraction

Indexed keywords

ANTINEOPLASTIC AGENT; CALCIUM; CALMODULIN; EGTAZIC ACID; FENDILINE; KAR 2; N (3,3 DIPHENYLPROPYL) N' [1 (3,4 DI N BUTOXYPHENYL)ETHYL] 1,3 DIAMINOPROPANE; UNCLASSIFIED DRUG;

ARTICLE; CIRCULAR DICHROISM; CONTROLLED STUDY; CRYSTALLIZATION; DRUG PROTEIN BINDING; PRIORITY JOURNAL; PROTEIN CONFORMATION; X RAY DIFFRACTION;

EID: 0343035681     PISSN: 08873585     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0134(199705)28:1<131::AID-PROT13>3.0.CO;2-K     Document Type: Article

References (25)

1. 2+ and target proteins. In: "Calmodulin." Cohen, P., Klee, C.B. (eds.), New York, Elsevier, 1988:35-56.
2. Crivici, A., Ikura, M. Molecular and structural basis of target recognition by calmodulin. Annu. Rev. Biophys. Biomol. Struct. 24:85-115, 1995.
3. Babu, Y. S., Bugg, C. E., Cook, W. J. Three-dimensional structure of calmodulin refined at 2.2 Å resolution. J. Mol. Biol. 204:191-204, 1988.
4. Chattopadhyaya, R., Meador, W.E., Means, A.R., Quiocho, F.A. Calmodulin structure refined at 1.7 Å resolution. J. Mol. Biol. 228:1177-1192, 1992.
5. Taylor, D.A., Sack, J.S., Manne, J.F., Beckingham, K., Quiocho, F.A. Structure of a recombinant calmodulin from Drosophila melanogaster refined at 2.2 Å resolution. J. Biol. Chem. 266:21375-21380, 1991.
6. 15N relaxation using inverse detected two-dimensional NMR spectroscopy: the central helix is flexible. Biochemistry 31:5269-5278, 1992.
7. Heidorn, D.B., Trewhella, J. Comparison of crystal and solution structures of calmodulin and troponin C. Biochemistry 27:909-915, 1988.
8. Ikura, M., Clore, G.M., Gronenborn, A.M., Zhu, G., Klee, C.B., Bax, A. Solution structure of a calmodulin-target peptide complex by multidimensional NMR. Science 256: 632-638, 1992.
9. Meador, W.E., Means, A.R., Quiocho, F.A. Target enzyme recognition by calmodulin: 2.4 Å structure of a calmodulin-peptide complex. Science 257:1251-1255, 1992.
10. Meador, W.E., Means, A.R., Quiocho, F.A. Modulation of calmodulin plasticity in molecular recognition on the basis of X-ray structures. Science 262:1718-1721, 1993.
11. Veigl, M.L., Klevit, R.E., Sedwick, W.D. The uses and limitations of calmodulin antagonists. Pharmacol. Ther. 44:181-239, 1989.
12. Ovádi, J. Effects of drugs on calmodulin-mediated enzymatic actions. Progr. Drug Res. 33:353-395, 1989.
13. Watabene, K., West, U. Effects of vinca alkaloids on calcium-calmodulin regulated cyclic adenosine 3′,5′-monophosphodiesterase activity from brain. Biochem. Pharmacol. 30:335-340, 1981.
14. Molnár, A., Liliom, K., Orosz, F., Vertessy, B.G., Ovádi, J. Anti-calmodulin potency of indol alkaloids in in vitro systems. Eur. J. Pharmacol. 291:73-82, 1995.
15. Cook, W.J., Walter, L.J., Walter, M.R. Drug binding by calmodulin: crystal structure of a calmodulin-trifluoperazine complex. Biochemistry 33:15259-15265, 1994.
16. 2+-calmodulin. Nature Struct. Biol. 1:795-801, 1994.
17. Kawasaki, H., Itoh, S., Kasai, H., Mitsui, Y., Iitaka, Y., Nakanashi, M., Okuyama, T. Crystallization of calcium-calmodulin-trifluperazine complex and an attempt at crystallizing calcium-free calmodulin. J. Biochem. 97:1815-1818, 1985.
18. Orosz, F., Christova, T.Y., Ovádi, J. Functional in vitro test of calmodulin antagonism: effect of drugs on interaction between calmodulin and glycolytic enzymes. Mol. Pharmacol. 33:678-682, 1988.
19. Ovádi, J., Keve, T., Acs, T., Orosz, F., Lehotzky, A., Liliom, K., Molnár, A., Nuridsány, M., Hlavanda, E., Vertessy, B.G., Kovács, J., Löw, M. New anti-tumor bis-indol derivatives with calmodulin liberator effect and pharmaceutical compositions containing them. Hung. Patent Descript. #978/95, 1995.
20. Kuboniwa, H., Tjandra, N., Grzesiek, S., Ren, H., Klee, C.B., Bax, A. Solution structure of calcium-free calmodulin. Nature Struct. Biol. 2:768-776, 1995.
21. Zhang, M., Tanaka, T., Ikura, M. Calcium-induced conformational transition revealed by the solution structure of apocalmodulin. Nature Struct. Biol. 2:758-767, 1995.
22. Finn, B.E., Evenas, J., Drakenberg, T., Waltho, J.P., Thulin, E., Forsen, S. Calcium-induced structural changes and domain autonomy in calmodulin. Nature Struct. Biol. 2:777-783, 1995.
23. 2+-induced hydrophobic site on calmodulin: application for purification of calmodluin by phenyl-sepharose affinity chromatography. Biochem. Biophys. Res. Commun. 104:830-836, 1982.
24. Hennessey, J.P. Jr., Manavalan, P., Johnson, W.C. Jr., Malencik, D.A., Anderson, S.R., Schimerlik, M.I., Shalitin, Y. Conformational transitions of calmodulin as studied by vacuum-UV CD. Biopolymers 26:561-571, 1987.
25. 2+ is absent. J. Biol. Chem. 270:6741-6750, 1995.