Mitochondrial carrier proteins can reversibly change their transport mode: The cases of the aspartate/glutamate and the phosphate carrier

Experimental Physiology

Volumn 83, Issue 2, 1998, Pages 259-265

  Krämer, Reinhard ^a,b  

^a FORSCHUNGSZENTRUM JÜLICH   (Germany)

^b UNIVERSITY OF COLOGNE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIPORTER; ASPARTIC ACID; CARRIER PROTEIN; CYSTEINE; GLUTAMIC ACID; LIPOSOME; PHOSPHATE; SERINE;

AMINO ACID TRANSPORT; CONFERENCE PAPER; ELECTROPHYSIOLOGY; ESCHERICHIA COLI; MITOCHONDRIAL MEMBRANE; MITOCHONDRION; NONHUMAN; PATCH CLAMP; SACCHAROMYCES CEREVISIAE;

EID: 0343028646     PISSN: 09580670     EISSN: None     Source Type: Journal    
DOI: 10.1113/expphysiol.1998.sp004111     Document Type: Conference Paper

References (26)

1. AQUILA, H., LINK, T. A. & KLINGENBERG, M. (1987). Solute carriers involved in energy transfer of mitochondria form a homologous protein family. FEBS Letters 212, 1-9.
2. 2+. Biochemistry 35, 8483-8488.
3. CAMMACK, J. N. & SCHWARTZ, E. A. (1996). Channel behavior in a gamma-aminobutyrate transporter. Proceedings of the National Academy of Sciences of the USA 93, 723-727.
4. DEFELICE, L. J. & BLAKELY, R. D. (1996). Pore models for transporters? Biophysical Journal 70, 579-580.
5. DIERKS, T. & KRÄMER, R. (1988). Asymmetric orientation of the reconstituted aspartate/glutamate carrier from mitochondria. Biochimica et Biophysica Acta 937, 112-126.
6. DIERKS, T., SALENTIN, A., HEBERGER, C. & KRÄMER, R. (1990a). The mitochondrial aspartate/glutamate and ADP/ATP carrier switch from obligate counterexchange to unidirectional transport after modification by SH-reagents. Biochimica et Biophysica Acta 1028, 268-280.
7. DIERKS, T., SALENTIN, A. & KRÄMER, R. (1990b). Pore-like and carrier-like properties of the mitochondrial aspartate/glutamate carrier after modification by SH-reagents: evidence for a preformed channel as a structural requirement of carrier-mediated transport. Biochimica et Biophysica Acta 1028, 281-288.
8. HERICK, K. & KRÄMER, R. (1995). Kinetic and energetic characterization of solute flux through the reconstituted aspartate/glutamate carrier from beef heart mitochondria after modification with mercurials. Biochimica et Biophysica Acta 1238, 63-71.
9. HERICK, K., KRÄMER, R. & LÜHRING, H. (1997). Patch clamp investigation into the phosphate carrier from Saccharomyces cerevisiae mitochondria. Biochimica et Biophysica Acta 1321, 207-220.
10. HUANG, S. G. & KLINGENBERG, M. (1997). Chloride channel properties of the uncoupling protein from brown adipose tissue mitochondria: a patch clamp study. Biochemistry 35, 16806-16814.
11. INDIVERI, C., TONAZZI, A. & PALMIERI, F. (1991). Characterization of the unidirectional transport of carnitine catalyzed by the reconstituted carnitine carrier from rat liver mitochondria. Biochimica et Biophysica Acta 1069, 110-116.
12. JENCKS, W. P. (1980). The utilization of binding energy in coupled vectorial processes. Advances in Enzymology 51, 75-106.
13. KLINGENBERG, M. (1991). Mechanistic and energetic aspects of carrier catalysis - exemplified with mitochondrial translocators. In A Study of Enzymes, vol. 2, Mechanism of Enzyme Action, ed. KUBY, S. A., pp. 367-389. CRC Press, Boca Raton.
14. KRÄMER, R. (1994). Functional principles of solute transport systems - concepts and perspectives. Biochimica et Biophysica Acta 1185, 1-34.
15. KRUPKA, R. M. (1993a). Coupling mechanisms in active transport. Biochimica et Biophysica Acta 1183, 105-113.
16. KRUPKA, R. M. (1993b). Coupling mechanisms in ATP-driven pumps. Biochimica et Biophysica Acta 1183, 114-122.
17. KRUPKA, R. M. (1995). Interpreting the effects of specific protein modification on antiport coupling mechanisms: The case of the aspartate/glutamate exchanger. Biochimica et Biophysica Acta 1236, 1-9.
18. NIKAIDO, H. & SAIER, M. H. (1992). Transport proteins in bacteria - common themes in their design. Science 258, 936-942.
19. PHELPS, A. & WOHLRAB, H. (1991) Mitochondrial phosphate transport - the Saccharomyces cerevisiae (threonine-43 to cysteine) mutant protein explicitly identifies transport with genomic sequence. Journal of Biological Chemistry 266, 19882-19885.
20. RUNSWICK, M. J., POWELL, S. J., NYREN, P. & WALKER, J. E. (1987). Sequence of the bovine mitochondrial phosphate carrier protein: structural relationship to ADP/ATP translocase and the brown fat mitochondria uncoupling protein. EMBO Journal 6, 1367-1373.
21. SCHROERS, A., KRÄMER, R. & WOHLRAB, H. (1997). The reversible antiport-uniport conversion of the phosphate carrier from yeast mitochondria depends on the presence of a single cysteine. Journal of Biological Chemistry 272, 10558-10564.
22. SCHWARZ, M., GROSS, A., STEINKAMP, T., FLÜGGE, U. I. & WAGNER, R. (1994). Ion channel properties of the reconstituted chloroplast triose phosphate/phosphate translocator. Journal of Biological Chemistry 269, 29481-29489.
23. STAPFEN, R. & KRÄMER, R. (1993). Functional properties of the reconstituted phosphate carrier from bovine heart mitochondria - evidence for asymmetric orientation and characterization of 3 different transport modes. Biochimica et Biophysica Acta 1149, 40-48.
24. - antiports catalyzed by reconstituted phosphate carrier from beef heart mitochondria. Journal of Biological Chemistry 269, 11240-11246.
25. TIKHONOVA, I. M., ANDREYEV, A. Y., ANTONENKO, Y. N., KAULEN, A. D., KOMRAKOV, A. Y. & SKULACHEV, V. P. (1994). Ion permeability induced in artificial membranes by the ATP/ADP antiporter. FEBS Letters 337, 231-234.
26. WOHLRAB, H. & BRIGGS, C. (1994). Yeast mitochondrial phosphate transport protein expressed in Escherichia coli. Site directed mutations at threonine-43 and at a similar location in the second tandem repeat (isoleucine-141). Biochemistry 33, 9371-9375.