Methods for analysis of Ca2+/H+ antiport activity in synaptic vesicles isolated from sheep brain cortex

Brain Research Protocols

Volumn 5, Issue 1, 2000, Pages 102-108

  Gonçalves, Paula P ^a   Meireles, Sandra M ^a   Neves, Paulo ^a   Vale, M Graça P ^b  

^a UNIVERSITY OF AVEIRO   (Portugal)

^b UNIVERSITY OF COIMBRA   (Portugal)

Author keywords

Brain cortex; Ca2+ uptake; Ca2+ H+ antiport; Synaptic vesicle

Indexed keywords

CALCIUM 45; CALCIUM ION; PROTON; PROTON PUMP;

ANIMAL TISSUE; ARTICLE; BRAIN CORTEX; CALCIUM TRANSPORT; CONTROLLED STUDY; ION TRANSPORT; MALE; NONHUMAN; PH; PRIORITY JOURNAL; SHEEP; SYNAPSE VESICLE; TECHNIQUE; TIME;

ACRIDINE ORANGE; ADENOSINE TRIPHOSPHATE; ANIMALS; ANTI-BACTERIAL AGENTS; ANTIPORTERS; BIOLOGICAL TRANSPORT; CALCIUM; CALCIUM-BINDING PROTEINS; CATION TRANSPORT PROTEINS; CELL FRACTIONATION; CEREBRAL CORTEX; ELECTROCHEMISTRY; ENZYME INHIBITORS; FLUORESCENT DYES; HYDROGEN-ION CONCENTRATION; MACROLIDES; MAGNESIUM CHLORIDE; MALE; PROTONS; SHEEP; SYNAPTIC VESICLES;

EID: 0342905402     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-299X(99)00061-6     Document Type: Article

References (30)

1. 2+ and the regulation of neurotransmitter secretion. Curr. Opin. Neurobiol. 7:1997;316-322.
2. Berridge M.J. Neuronal calcium signaling. Neuron. 21:1998;13-26.
3. + antiport in isolated tonoplast vesicles from storage tissue of Beta vulgaris L. Plant Physiol. 80:1986;727-731.
4. Burger P.M., Mehl E., Cameron P.L., Maycox P.R., Baumert M., Lottspeich F., De Camilli P., Jahn R. Synaptic vesicles immunoisolated from rat cerebral cortex contain high levels of glutamate. Neuron. 3:1989;715-720.
5. Deamer D.W., Prince R.C., Crofts A.R. The response of fluorescent amines to pH gradients across liposome membranes. Biochim. Biophys. Acta. 274:1972;323-335.
6. Floor E., Schaeffer S.F., Feist B.E., Leeman S.E. Synaptic vesicles from mammalian brain: large-scale purification and physical and immunochemical characterization. J. Neurochem. 50:1988;1588-1596.
7. Fossier Ph., Tauc L., Baux G. Calcium transients and neurotransmitter release at an identified synapse. Trends Neurosci. 22:1999;161-166.
8. 2+-ATPase. Biochem. J. 276:1991;363-367.
9. Fykse E.M., Fonnum F. Uptake of γ-aminobutyric acid by synaptic vesicle fraction isolated from rat brain. J. Neurochem. 50:1988;1237-1242.
10. +-Antiport activity in synaptic vesicles isolated from sheep brain cortex. Neurosci. Lett. 247:1998;87-90.
11. + antiport in synaptic vesicles of sheep brain cortex. Mol. Brain Res. 67:1999;283-291.
12. + antiport: dependence on the proton electrochemical gradient. Mol. Brain Res. 71:1999;178-184.
13. Gornall A.G., Bardawill Ch.J., David M.M. Determination of serum proteins by means of the biuret reaction. J. Biol. Chem. 177:1949;751-766.
14. Hell J.W., Maycox P.R., Stadler H., Jahn R. Uptake of GABA by rat brain synaptic vesicles isolated by a new procedure. EMBO J. 7:1988;3023-3029.
15. Hell J.W., Maycox P.R., Jahn R. Energy dependence and functional reconstitution of γ-aminobutyric acid carrier from synaptic vesicles. J. Biol. Chem. 265:1990;2111-2117.
16. Huttner W.B., Schiebler P., Greengard P., De Camilli P. Synapsin I (protein I), a nerve terminal-specific phosphoprotein: III. Its association with synaptic vesicles studied in a highly purified synaptic vesicle preparation. J. Cell Biol. 96:1983;1374-1388.
17. Kish P.E., Ueda T. Glutamate accumulation into synaptic vesicles. Methods Enzymol. 174:1989;9-25.
18. Kostyuk P., Verkhratsky A. Calcium stores in neurons and glia. Neuroscience. 63:1994;381-404.
19. Marquez-Sterling N., Lo A.C.Y., Sisodia S.S., Koo E.H. Trafficking of cell-surface β-amyloid precursor protein: evidence that a sorting intermediate participates in synaptic vesicle recycling. J. Neurosci. 17:1997;140-151.
20. 2+ transport into cholinergic Torpedo synaptic vesicles. J. Neurochem. 35:1980;116-124.
21. Mizuhira V., Hasegawa H. Microwave fixation and localization of calcium in synaptic terminals using X-ray microanalysis and electron energy loss spectroscopy imaging. Brain Res. Bull. 43:1997;53-58.
22. Moriyama Y., Tsai H.L., Futai M. Energy-dependent accumulation of neuron blockers causes selective inhibition of neurotransmitter uptake by brain synaptic vesicles. Arch. Biochem. Biophys. 305:1993;278-281.
23. Parducz A., Dunant Y. Transient increase of calcium in synaptic vesicles after stimulation. Neuroscience. 52:1993;27-33.
24. 2+ be released from secretory granules or synaptic vesicles? Trends Neurosci. 19:1996;411-413.
25. Shioi J., Ueda T. Artificially imposed electrical potentials drive L-glutamate uptake into synaptic vesicles of bovine cerebral cortex. Biochem. J. 267:1990;63-68.
26. Taussky H.H., Shorr E. A microcolorimetric method for the determination of the inorganic phosphorus. J. Biol. Chem. 202:1953;675-685.
27. + antiport in plasma membrane vesicles of corn roots. J. Exp. Botany. 46:1995;1551-1559.
28. Webb M.R. A continuous spectrophotometric assay for inorganic phosphate and for measuring phosphate release kinetics in biological systems. Proc. Natl. Acad. Sci. U.S.A. 89:1992;4884-4887.
29. Whittaker V.P., Michaelson J.A., Kirkland R.J.A. The separation of synaptic vesicles from nerve-endings particles (synaptosomes). Biochem. J. 90:1964;293-303.
30. Wolosker H., Reis M., Assreuy J., Meis L. Inhibition of glutamate uptake and proton pumping in synaptic vesicles by S-nitrosylation. J. Neurochem. 66:1996;1943-1948.