Receptor subtype-induced targeting and subtype-specific internalization of human α2-adrenoceptors in PC12 cells

Journal of Neuroscience

Volumn 19, Issue 21, 1999, Pages 9281-9288

  Olli Lähdesmäki, Tuire ^a,b   Kallio, Jaana ^a,b   Scheinin, Mika ^a,b  

^a UNIVERSITY OF TURKU   (Finland)

^b UNIVERSITY OF TURKU   (Finland)

Author keywords

Differentiation; Endocytosis; Immunocytochemistry; Internalization; PC12 cells; Targeting; 2 adrenoceptors

Indexed keywords

ALPHA 2 ADRENERGIC RECEPTOR; RECEPTOR SUBTYPE;

ANIMAL CELL; ARTICLE; CELL COMPARTMENTALIZATION; CELL DIFFERENTIATION; DESENSITIZATION; ENDOCYTOSIS; GENE TARGETING; IMMUNOCYTOCHEMISTRY; IMMUNOFLUORESCENCE; INTERNALIZATION; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; RAT; RECEPTOR UPREGULATION; TARGET CELL; TISSUE DISTRIBUTION;

EID: 0342858831     PISSN: 02706474     EISSN: None     Source Type: Journal    
DOI: 10.1523/jneurosci.19-21-09281.1999     Document Type: Article

References (57)

1. 2-Adrenoceptor stimulation promotes actin polymerization and focal adhesion in 3T3F442A and BFC-1β preadipocytes. Endocrinology 137:5220-5229.
2. Chen CA, Okayama H (1987) High-efficiency transformation of mammalian cells by plasmid DNA. Mol Cell Biol 7:2745-2752.
3. Chen CA, Okayama H (1988) Calcium phosphate-mediated gene transfer: a highly efficient transfection system for stably transforming cells with plasmid DNA. Biotechniques 6:632-638.
4. Chruscinski AJ, Link RE, Daunt DA, Barsh GS, Kobilka BK (1992) Cloning and expression of the mouse homolog of the human α2-C2 adrenergic receptor. Biochem Biophys Res Commun 186:1280-1287.
5. Daunt AD, Hurt C, Hein L, Kallio J, Feng F, Kobilka BK (1997) Subtype-specific intracellular trafficking of α2-adrenergic receptors. Mol Pharmacol 51:711-720.
6. 2-adrenergic receptors. J Biol Chem 267:25473-25479.
7. 2-adrenergic receptor-Gs coupling undergoes agonist-promoted desensitization in a subtype-selective manner. Biochem Biophys Res Commun 193:318-323.
8. Greene LA, Tischler AS (1976) Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc Natl Acad Sci USA 73:2424-2428.
9. Greene LA, Sobeih MM,Teng KK (1991) Methodologies for the culture and experimental use of the PC12 rat pheochromocytoma cell line. In: Culturing nerve cells (Banker G, Goslin K, eds), pp 207-226. Cambridge, MA: MIT.
10. 3H]RX821002 as radioligands. Biochim Biophys Acta 1266:207-214.
11. Karnovsky MJ (1971) Use of ferrocyanide-reduced osmium tetroxide in electron microscopy. Proceedings of the 11th Annual Meeting of the American Society of Cell Biology.
12. Keefer JR, Kennedy ME, Limbird LE (1994) Unique structural features important for stabilization versus polarization of the α2A-adrenergic receptor on the basolateral membrane of Madin-Darby canine kidney cells. J Biol Chem 269:16425-16433.
13. + channels by interaction with a family of membrane-associated guanylate kinases. Nature 378:85-88.
14. Kobilka BK, Matsui H, Kobilka TS, Yang-Feng TL, Francke U, Caron MG, Lefkowitz RJ, Regan JW (1987) Cloning, sequencing, and expression of the gene coding for the human platelet α2-adrenergic receptor. Science 238:650-656.
15. Koenig JA, Edwardson JM (1997) Endocytosis and recycling of G protein-coupled receptors. Trends Pharmacol Sci 18:276-287.
16. Kornau HC, Schenker LT, Kennedy MB, Seeburg PH (1995) Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95. Science 269:1737-1740.
17. Kurose H, Lefkowitz RJ (1994) Differential desensitization and phosphorylation of three cloned and transfected α2-adrenergic receptor subtypes. J Biol Chem 269:10093-10099.
18. 2a-adrenergic receptor via "hit and run" gene targeting reveals the role of this subtype in sedative, analgesic, and anesthetic-sparing responses in vivo. Proc Natl Acad Sci USA 94:9950-9955.
19. Lanier SM, Downing S, Duzic E, Homcy CJ (1991) Isolation of rat genomic clones encoding subtypes of the α2-adrenergic receptor. J Biol Chem 266:10470-10478.
20. Lawhead RG, Blaxall HS, Bylund DB (1992) α2A is the predominant α2 adrenergic receptor subtype in human spinal cord. Anesthesiology 77:983-991.
21. Lee V, Shelanski ML, Greene LA (1977) Specific neural and adrenal medullary antigens detected by antisera to clonal PC12 and pheochromocytoma cells. Proc Natl Acad Sci USA 74:5021-5025.
22. Lee VM, Shelanski ML, Greene LA (1980) Characterization of antisera raised against cultured rat sympathetic neurons. J Neurosci 5:2239-2245.
23. Liitti S, Närvä H, Marjamäki A, Hellman J, Kallio J, Jalkanen M, Matikainen MT (1997) Subtype specific recognition of human alpha2C2 adrenergic receptor using monoclonal antibodies against the third intracellular loop. Biochem Biophys Res Commun 233:166-172.
24. 2C10 homolog responsible for an interspecies variation in antagonist binding. Mol Pharmacol 42:16-27.
25. Link RE, Desai K, Hein L, Stevens ME, Chruscinski A, Bernstein D, Barsh GS, Kobilka BK (1996) Cardiovascular regulation in mice lacking α2-adrenergic receptor subtypes B and C. Science 273:803-805.
26. 2-adrenergic receptor subtype, the gene for which is located on chromosome 2. Proc Natl Acad Sci USA 87:5094-5098.
27. MacDonald E, Kobilka BK, Scheinin M (1997) Gene targeting-homing in on α2-adrenoceptor subtype function. Trends Pharmacol Sci 18:211-219.
28. 2a-adrenergic receptor subtype. Science 373:801-803.
29. 3H]rauwolscine binding, inhibition of adenylate cyclase and RNase protection assay. Biochim Biophys Acta 1134:169-177.
30. Marjamäki A, Luomala K, Ala-Uotila S, Scheinin M (1993) Use of recombinant human α2-adrenoceptors to characterize subtype selectivity of antagonist binding. Eur J Pharmacol 246:219-226.
31. 2+/calmodulin-dependent protein kinase II in PC12 cells alters cell growth, morphology, and nerve growth factor-induced differentiation. J Neurosci 17:924-931.
32. McCune SK, Voigt MM, Hill JM (1993) Expression of multiple alpha adrenergic receptor subtype messenger RNAs in the adult rat brain. Neuroscience 57:143-151.
33. Milner TA, Rosin DL, Lee A, Aicher SA (1999) Alpha2A-adrenergic receptors are primarily presynaptic heteroreceptors in the C1 area of the rat rostral ventrolateral medulla. Brain Res 821:200-211.
34. Nicholas AP, Pieribone V, Hökfelt T (1993) Distributions of mRNAs for alpha-2 adrenergic receptor subtypes in rat brain: an in situ hybridization study. J Comp Neurol 328:575-594.
35. Nicholas AP, Hökfelt T, Pieribone VA (1996) The distribution and significance of CNS adrenoceptors examined with in situ hybridization. Trends Pharmacol Sci 17:245-255.
36. Ok usa MD, Lynch KR, Rosin DL, Huang L, Wei YY (1994) Apical membrane and intracellular distribution of endogenous alpha2A-adrenergic receptors in MDCK cells. Am J Physiol 267:F347-F353.
37. Regan JW, Kobilka TS, Yang-Feng TL, Caron MG, Lefkowitz RJ, Kobilka BK (1988) Cloning and expression of human kidney cDNA for an adrenergic receptor subtype. Proc Natl Acad Sci USA 85:6301-6305.
38. Rosin DL, Zeng D, Stornetta RL, Norton FR, Riley T, Okusa MD, Guyenet PG, Lynch KRN (1993) Immunohistochemical localization of alpha2A-adrenergic receptors in catecholaminergic and other brainstem neurons in the rat. Neuroscience 56:139-155.
39. 2C-adrenergic receptor-like immunoreactivity in the rat central nervous system. J Comp Neurol 372:135-165.
40. Sallinen J, Link RE, Haapalinna A, Viitamaa T, Kulatunga M, Sjöholm B, MacDonald E, Pelto-Huikko M, Leino T, Barsh GS, Kobilka BK, Scheinin M (1997) Genetic alteration of alpha 2C-adrenoceptor expression in mice: influence on locomotor, hypothermic, and neurochemical effects of dexmedetomidine, a subtype-nonselective alpha 2-adrenoceptor agonist. Mol Pharmacol 51:36-46.
41. 2C-adrenergic receptor subtype. Neuroscience 86:959-965.
42. 2C-receptors modulate the acoustic startle reflex, prepulse inhibition and aggression in mice. J Neurosci 18:3035-3042.
43. Saunders C, Keefer JR, Bonner CA, Limbird LE (1998) Targeting of G-protein-coupled receptors to the basolateral surface of polarized renal epithelial cells involves multiple, non-contiguous structural signals. J Biol Chem 273:24196-24206.
44. 2-adrenergic receptor subtype gene expression in rat brain. Brain Res Mol Brain Res 21:133-149.
45. Stempak JG, Ward RT (1964) An improved staining method for electron microscopy. J Cell Biol 22:697-701.
46. 2 agonists and is necessary for spinal adrenergic-opioid synergy. J Neurosci 17:7157-7165.
47. Talley EM, Rosin DL, Lee A, Guyenet PG, Lynch KR (1996) Distribution of alpha 2A-adrenergic receptor-like immunoreactivity in the rat central nervous system. J Comp Neurol 372:111-134.
48. Venable JH, Coggeshall R (1965) A simplified lead citrate stain for use in electron microscopy. J Cell Biol 25:407-408.
49. Vickery RG, von Zastrow M (1999) Distinct dynamin-dependent and -independent mechanisms target structurally homologous dopamine receptors to different endocytic membranes. J Cell Biol 144:31-43.
50. von Zastrow M, Link R, Daunt D, Barsh D, Kobilka B (1993) Subtype-specific differences in the intracellular sorting of G-protein-coupled receptors. J Biol Chem 268:763-766.
51. 2-adrenergic receptor gene in transgenic animals. Neuroscience 74:199-218.
52. Winzer-Serhan UH, Leslie FM (1997) Alpha 2B adrenoceptor mRNA expression during rat brain development. Brain Res Dev Brain Res 100:90-100.
53. 2-adrenergic receptor subtypes achieve basolateral localization in Madin-Darby canine kidney II cells via different targeting mechanisms. J Biol Chem 271:5017-5024.
54. Wozniak M, Limbird LE (1998) Trafficking itineraries of G protein-coupled receptors in epithelial cells do not predict receptor localization in neurons. Brain Res 780:311-322.
55. Youdim MB, Heldman E, Pollard HB, Fleming P, McHugh E (1986) Contrasting monoamine oxidase activity and tyramine induced catecholamine release in PC12 and chromaffin cells. Neuroscience 19:1311-1318.
56. Zeng DW, Harrison JK, D'Angelo DD, Barber CM, Tucker AL, Lu ZH, Lynch KR (1990) Molecular characterization of a rat α2B-adrenergic receptor. Proc Natl Acad Sci USA 87:3102-3106.
57. Zubiaur M, Neer EJ (1993) Nerve growth factor changes G protein levels and localization in PC12 cells. J Neurosci Res 35:207-217.