The catalytic amino-acid residues in the active site of cellobiohydrolase 1 are involved in chiral recognition

Journal of Biotechnology

Volumn 57, Issue 1-3, 1997, Pages 115-125

  Henriksson, Hongbin ^a   Ståhlberg, Jerry ^a   Koivula, Anu ^b   Pettersson, Göran ^a   Divne, Christina ^a   Valtcheva, Ludmila ^a   Isaksson, Roland ^c  

^a UPPSALA UNIVERSITY   (Sweden)

^b VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

^c UPPSALA UNIVERSITY   (Sweden)

Author keywords

Cellobiohydrolase 1; Chiral recognition mechanism; Chiral stationary phase; Endoglucanase 1; Protein engineering

Indexed keywords

AMINO ACIDS; CATALYST ACTIVITY; CATALYST SELECTIVITY; DRUG PRODUCTS; ENZYME IMMOBILIZATION; MUTAGENESIS; SILICA;

CELLOBIOHYDROLASE; CHIRAL RECOGNITION; CHIRAL STATIONARY PHASE; ENANTIOSELECTIVITY; PROTEIN ENGINEERING;

ENZYMES;

CELLULOSE; CELLULOSE 1,4 BETA CELLOBIOSIDASE; GLUCAN SYNTHASE; MEXILETINE; OLIGOSACCHARIDE;

ARTICLE; BIODEGRADATION; CATALYSIS; CHIRALITY; CONTROLLED STUDY; GENETIC ENGINEERING; MUTANT; NONHUMAN; PRIORITY JOURNAL; TRICHODERMA REESEI;

EID: 0342813138     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(97)00094-1     Document Type: Article

References (26)

1. Abuja, P.M., Schmuck, M., Pilz, I., Tomme, P., Claeyssens, M., Esterbauer, H., 1988. Structural and functional domains of cellobiohydrolase I from Trichoderma reesei. A small angle X-ray study of the intact enzyme and its core. Eur. Biophys. J. 15, 339-342.
2. Barr, B.K., Hsieh, Y.-L., Ganem, B., Wilson, D.B., 1996. Identification of two functionally distinct classes of exocellulases. Biochemistry 35, 586-592.
3. Bhikhabhai, R., Johansson, G., Pettersson, G., 1984. Isolation of cellulolytic enzymes from Trichoderma reesei QM 9414. J. Anal. Biochem. 6, 336-345.
4. Claeyssens, M., Tomme, P., Brewer, C.F., Hehre, E.J., 1990. Stereochemical course of hydrolysis and hydration reactions catalysed by cellobiohydrolases I and II from Trichoderma reesei. FEBS Lett. 263, 89-92.
5. Davies, G., Henrissat, B., 1995. Structures and mechanisms of glycosyl hydrolases. Structure 3, 853-859.
6. Divne, C., Ståhlberg, J., Reinikainen, T., Ruohonen, L., Pettersson, G., Knowles, J.K.C., Teeri, T.T., Jones, A., 1994. The three-dimensional structure of the catalytic core of cellobiohydrolase I from Trichoderma reesei. Science 265, 524-528.
7. Henriksson, H., Jönsson, S., Isaksson, R., Pettersson, G., 1995. Chiral separation based on intact and fragmented CBH 2: A comparison with CBH 1. Chirality 7, 415-424.
8. Henriksson, H., Ståhlberg, J., Isaksson, R., Pettersson, G., 1996. The active sites of cellulases are involved in chiral recognition: a comparison of cellobiohydrolase I and endoglucanase 1. FEBS Lett. 390, 339-344.
9. Henrissat, B., 1991. A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J. 280, 309-316.
10. Henrissat, B., Bairoch, A., 1993. New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J. 293, 781-788.
11. Hjertén, S., Li, Y.-M., Liao, J.-L., Nakazato, K., Pettersson, G., 1992. Continuous beds: high-resolving, cost-effective, chromatography matrices. Nature, Product Review 356, 810.
12. Isaksson, R., Pettersson, C., Pettersson, G., Jönsson, S., Ståhlberg, J., Hermansson, J., Marle, I., 1994. Cellulases as chiral selectors. Trends Anal. Chem. 13 (10), 431-439.
13. Kaiser, R., 1960. Chromatographie in der Gasphase, I, Gaschromatographie, Bibliographisches Institut, Mannheim, Ch. 2.
14. Knowles, J.K.C., Lehtovaara, P., Murray. M., Sinnott, M.L., 1988. Stereochemical course of the action of the cellobiohydrolases I and II of Trichoderma reesei. J. Chem. Soc. Chem. Commun. 1988, 1401-1402.
15. Marle, I., Erlandsson, P., Hansson, L., Isaksson, R., Pettersson, C., Pettersson, G., 1991. Separation of enantiomers using cellulase (CBH I) silica as a chiral stationary phase. J. Chromatogr. 586, 233-248.
16. Marle, I., Jönsson, S., Isaksson, R., Pettersson, C., Pettersson, G., 1993. Chiral stationary phases based on intact and fragmented cellobiohydrolase I immobilized on silica. J. Chromatogr. 648, 333-347.
17. McCarter, J.D., Withers, S.G., 1994. Mechanisms of enzymatic glycoside hydrolysis. Curr. Op. Struct. Biol. 4, 885-892.
18. Mohammad, J., Li, Y.-M., El-Ahmad, M., Nakazato, K., Pettersson, G., Hjertén, S., 1993. Chiral-resolution chromatography of β-blockers on continuous polymer beds with immobilized cellulase as enantioselective protein. Chirality 5, 464-470.
19. Penttilä, M., Lehtovaara, P., Nevalainen, H., Bhikhabhai, R., Knowles, J., 1986. Homology between cellulase genes of Trichoderma reesei: complete nucleotide sequence of the endo-glucanase I gene. Gene 45, 253-263.
20. Rouvinen, J., Bergfors, T., Teeri, T., Knowles, J.K.C., Jones, T.A., 1990. Three-dimensional structure of cellobiohydrolase II from Trichoderma reesei. Science 249, 380-386.
21. Ståhlberg, J., Divne, C., Koivula, A., Piens, K., Claeyssens, M., Teeri, T.T., Jones, T.A., 1996. Activity studies and crystal structures of catalytically deficient mutants of Cellobiohydrolase I from Trichoderma reesei. J. Mol. Biol. 264, 337-349.
22. Suominen, P.L., Mäntylä, A.L., Karhunen, T., Hakola, S., Nevalainen, H., 1993. II. Effect of deletions of individual cellulase genes. Mol. Gen. Genet. 241, 523-530.
23. Tomme, P., van Tilbeurgh, H., Pettersson, G., van Damme, J., Vandekerckhove, J., Knowles, J., Teeri, T., Claeyssens, M., 1988. Studies of the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis. Eur. J. Biochem. 170, 575-581.
24. Valtcheva, L., Mohammad, J., Pettersson, G., Hjertén, S., 1993. Chiral separation of β-blockers by high-performance capillary electrophoresis based on non-immobilized cellulase as enantioselective protein. J. Chromatogr. 638, 263-267.
25. Van Tilbeurgh, H., Tomme, P., Claeyssens, M., Bhikhabhai, R., Pettersson, L.G., 1986. Limited proteolysis of the cellobiohydrolase I from Trichoderma reesei. Separation of the functional domains.. FEBS Lett. 204, 223-227.
26. Vrsanská, M., Biely, P., 1992. The cellobiohydrolase I from Trichoderma reesei QM 9414: action on cello-oligosaccharides. Carbohydr. Res. 227, 19-27.