U4 small nuclear RNA genes of trypanosomes: Cloning of the Leptomonas seymouri gene and mutational analysis of core snRNP assembly

Gene

Volumn 247, Issue 1-2, 2000, Pages 77-86

  Bell, Mathias ^a   Wöhner, Rosa ^a   Bindereif, Albrecht ^a  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

Sm site; snRNP biogenesis; Trans splicing

Indexed keywords

CARRIER PROTEIN; MESSENGER RNA; RIBONUCLEOPROTEIN;

ARTICLE; GENE EXPRESSION REGULATION; GENE EXPRESSION SYSTEM; MOLECULAR CLONING; MUTATION; NUCLEOTIDE SEQUENCE; PHYLOGENY; PRIORITY JOURNAL; RNA SPLICING; SEQUENCE ANALYSIS; TRYPANOSOMA;

ANIMALS; AUTOANTIGENS; BASE SEQUENCE; BINDING SITES; CELL NUCLEUS; CLONING, MOLECULAR; DNA, PROTOZOAN; DNA, RECOMBINANT; GENE EXPRESSION; LEISHMANIA; MOLECULAR SEQUENCE DATA; MUTAGENESIS; MUTATION; NUCLEIC ACID CONFORMATION; PLASMIDS; RIBONUCLEOPROTEINS, SMALL NUCLEAR; RNA, SMALL NUCLEAR; SEQUENCE ANALYSIS, DNA; TRANSFECTION; TRYPANOSOMATINA;

LEISHMANIA TARENTOLAE; LEPTOMONAS SEYMOURI; PROTOZOA; TRYPANOSOMA; TRYPANOSOMATIDAE;

EID: 0342803562     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(00)00118-9     Document Type: Article

References (33)

1. Bangs J.D., Crain P.F., Hashizume T., McCloskey J.A., Boothroyd J.C. Mass spectrometry of mRNA cap4 from trypanosomatids reveals two novel nucleosides. J. Biol. Chem. 267:1992;9805-9815.
2. Bell M., Bindereif A. Cloning and mutational analysis of the Leptomonas seymouri U5 snRNA gene: function of the Sm site in core RNP formation and nuclear localization. Nucleic Acids Res. 27:1999;3986-3994.
3. Bellofatto V., Hartree D.E., Torres-Munoz J. Leptomonas seymouri as a model system for the analysis of gene expression in trypanosomatids. J. Parasitol. 79:1993;637-644.
4. Burge C.B., Tuschl T., Sharp P.A. Splicing of precursors to mRNAs by the spliceosomes. Gesteland R.F., Cech T.R., Atkins J.F. The RNA World. 1999;525-560 Cold Spring Harbor Press, Cold Spring Harbor, NY.
5. Cross M., Günzl A., Palfi Z., Bindereif A. Analysis of small nuclear ribonucleoproteins (RNPs) in Trypanosoma brucei: structural organization and protein components of the spliced leader RNP. Mol. Cell. Biol. 11:1991;5516-5526.
6. Cross M., Wieland B., Palfi Z., Günzl A., Röthlisberger U., Lahm H.-W., Bindereif A. The trans-spliceosomal U2 snRNP protein 40K of Trypanosoma brucei: cloning and analysis of functional domains reveals homology to a mammalian snRNP protein. EMBO J. 12:1993;1239-1248.
7. Dungan J.M., Watkins K.P., Agabian N. Evidence for the presence of a small U5-like RNA in active trans-spliceosomes of Trypanosoma brucei. EMBO J. 15:1996;4016-4029.
8. Geiduschek E.P. Transcription by RNA polymerase III. Ann. Rev. Biochem. 57:1988;873-914.
9. Goldring A., Michaeli S. The U6 snRNA-encoding gene of the monogenetic trypanosomatid Leptomonas collosoma. Gene. 156:1995;139-144.
10. Goncharov I., Xu Y.X., Zimmer Y., Sherman K., Michaeli S. Structure-function analysis of the trypanosomatid spliced leader RNA. Nucleic Acids Res. 26:1998;2200-2207.
11. Goncharov I., Palfi Z., Bindereif A., Michaeli S. Purification of the spliced leader ribonucleoprotein particle from Leptomonas collosoma revealed the existence of an Sm protein in trypanosomes. Cloning the SmE homologue. J. Biol. Chem. 274:1999;12 217-12 221.
12. Günzl A., Cross M., Bindereif A. Domain structure of U2 and U4/U6 small nuclear ribonucleoprotein particles from Trypanosoma brucei: identification of trans-spliceosomal specific RNA-protein interactions. Mol. Cell. Biol. 12:1992;468-479.
13. Guthrie C., Patterson B. Spliceosomal snRNAs. Annu. Rev. Genet. 22:1988;387-419.
14. Hartshorne T., Agabian N. RNA B is the major nucleolar trimethylguanosine-capped small nuclear RNA associated with fibrillarin and pre-rRNAs in Trypanosoma brucei. Mol. Cell. Biol. 13:1993;144-154.
15. He P., Bellofatto V. Structure of the Leptomonas seymouri trans-spliceosomal U2 snRNA-encoding gene, potential U2-U6 snRNA interactions conform to the cis-splicing counterpart. Gene. 165:1995;131-135.
16. Lücke S., Xu G.L., Palfi Z., Cross M., Bellofatto V., Bindereif A. Spliced leader RNA of trypanosomes: in vivo mutational analysis reveals extensive and distinct requirements for trans splicing and cap4 formation. EMBO J. 15:1996;4380-4391.
17. Lücke S., Klöckner T., Palfi Z., Boshart M., Bindereif A. Trans mRNA splicing in trypanosomes: cloning and analysis of a PRP8-homologous gene from Trypanosoma brucei provides evidence for a U5-analogous RNP. EMBO J. 16:1997;4433-4440.
18. Madhani H.D., Bordonne R., Guthrie C. Multiple roles for U6 snRNA in the splicing pathway. Genes Dev. 4:1990;2264-2277.
19. Mottram J., Perry K.L., Lizardi P.M., Lührmann R., Agabian N., Nelson R.G. Isolation and sequence of four small nuclear U RNA genes of Trypanosoma brucei subsp. brucei: identification of the U2, U4 and U6 RNA analogs. Mol. Cell. Biol. 9:1989;1212-1223.
20. Myslinski E., Branlant C. A phylogenetic study of U4 snRNA reveals the existence of an evolutionarily conserved secondary structure corresponding to 'free' U4 snRNA. Biochimie. 73:1991;17-28.
21. Nakaar V., Tschudi C., Ullu E. An unusual liaison: small nuclear and cytoplasmic RNA genes team up with tRNA genes in trypanosomatid protozoa. Parasitol. Today. 11:1995;225-228.
22. Nilsen T.W. RNA-RNA interactions in nuclear pre-mRNA splicing. Simons R.W., Grunberg-Manago M. RNA Structure and Function. 1998;279-307 Cold Spring Harbor Press, Cold Spring Harbor, NY.
23. Palfi Z., Günzl A., Cross M., Bindereif A. Affinity purification of Trypanosoma brucei small nuclear ribonucleoproteins reveals common and specific protein components. Proc. Natl. Acad. Sci. USA. 88:1991;9097-9101.
24. Schnare M.N., Gray M.W. A candidate U1 small nuclear RNA for trypanosomatid protozoa. J. Biol. Chem. 274:1999;23 691-23 694.
25. Schnare M.N., Gray M.W. Spliced leader-associated RNA from Crithidia fasciculata contains a structure resembling stem/loop II of U1 snRNA. FEBS Lett. 459:1999;215-217.
26. Staley J.P., Guthrie C. Mechanical devices of the spliceosome: motors, clocks, springs, and things. Cell. 92:1998;315-326.
27. Tschudi C., Ullu E. Destruction of U2, U4, or U6 small nuclear RNA blocks trans splicing in trypanosome cells. Cell. 61:1990;459-466.
28. Vankan P., McGuigan C., Mattaj I.W. Domains of U4 and U6 snRNAs required for snRNP assembly and splicing complementation in Xenopus oocytes. EMBO J. 9:1990;3397-3404.
29. Wersig C., Bindereif A. Reconstitution of functional mammalian U4 small nuclear ribonucleoprotein: Sm protein binding is not essential for splicing in vitro. Mol. Cell. Biol. 12:1992;1460-1468.
30. Wieland B., Bindereif A. Unexpected diversity in U6 snRNA sequences from trypanosomatids. Gene. 161:1995;129-133.
31. Xu G.-L., Wieland B., Bindereif A. Trans-spliceosomal U6 RNAs of Crithidia fasciculata and Leptomonas seymouri: deviation from the conserved ACAGAG sequence and potential base pairing with spliced leader RNA. Mol. Cell. Biol. 14:1994;4565-4570.
32. Xu Y.-X., Ben-Shlomo H., Michaeli S. The U5 RNA of trypanosomes deviates from the canonical U5 RNA: the Leptomonas collosoma U5 RNA and its coding gene. Proc. Natl. Acad. Sci. USA. 94:1997;8473-8478.
33. Yean S.L., Lin R.J. U4 small nuclear RNA dissociates from a yeast spliceosome and does not participate in the subsequent splicing reaction. Mol. Cell. Biol. 11:1991;5571-5577.