Size-fractionation of RNA by hot agarose electrophoresis

BioTechniques

Volumn 28, Issue 3, 2000, Pages 414-416

  Almeida, Teresa A ^a   Pérez, José A ^a   Pinto, Francisco M ^a  

^a UNIVERSIDAD DE LA LAGUNA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

RNA;

ARTICLE; COLORIMETRY; DENATURATION; ELECTROPHORETIC MOBILITY; FRACTIONATION; GEL ELECTROPHORESIS; NUCLEOTIDE SEQUENCE;

EID: 0342680141     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00283bm04     Document Type: Article

References (5)

1. Bailey, J.M. and N. Davison. 1976. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Anal. Biochem. 70:75-85.
2. Ingelbrecht, I.L., C.I. Mandelbaum and T.E. Mirkov. 1998. Highly sensitive northern hybridization using a rapid protocol for downward alkaline blotting of RNA. BioTechniques 25:420-425.
3. Lehrach, H., J. Diamond, J.M. Wozney and H. Boedtker. 1977. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry 16:4743-4751.
4. Mc Master, G.K. and G.G. Carmichael. 1977. Analysis of single-and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange Proc. Natl. Acad. Sci. USA 74:4835-4838.
5. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. CSH Laboratory Press, Cold Spring Harbor, NY.