Detection of opioid receptor mRNA by RT-PCR reveals alternative splicing for the δ- and κ-opioid receptors

Molecular Brain Research

Volumn 48, Issue 2, 1997, Pages 298-304

  Gavériaux Ruff, Claire ^a   Peluso, Jean ^a   Befort, Katia ^a   Simonin, Frédéric ^a   Zilliox, Christelle ^a   Kieffer, Brigitte L ^a  

^a UNIVERSITÉ DE STRASBOURG   (France)

Author keywords

Opioid receptor; Opioid receptor; Brain; G protein coupled receptor; Immune cells; Opioid receptor; Polymerase chain reaction

Indexed keywords

DELTA OPIATE RECEPTOR; KAPPA OPIATE RECEPTOR; MESSENGER RNA;

ALTERNATIVE RNA SPLICING; ANIMAL TISSUE; ARTICLE; BRAIN; CELL LINE; DNA SEQUENCE; EXON; GENE DELETION; GENE INSERTION; MONOCYTE; MOUSE; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; T LYMPHOCYTE;

ALTERNATIVE SPLICING; AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CELL LINE; DNA, COMPLEMENTARY; EXONS; GENOME; HUMANS; MICE; MICE, INBRED C57BL; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RECEPTORS, OPIOID, DELTA; RECEPTORS, OPIOID, KAPPA; RNA, MESSENGER; TRANSCRIPTION, GENETIC;

ANIMALIA;

EID: 0342617499     PISSN: 0169328X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0169-328X(97)00109-5     Document Type: Article

References (30)

1. Augustin LB, Flesheim RF, Min BH, Fuchs SM, Fuchs JA, Loh HH Genomic structure of the mouse delta opioid receptor gene. Biochem. Biophys. Res. Commun. 207:1995;111-119.
2. Bare LA, Mansson E, Yang DM Expression of two variants of the human mu opioid receptor mRNA in SK-N-SH cells and human brain. FEBS Lett. 354:1994;213-216.
3. Borboni P, Di Cola G, Sesti Get al. β-Endorphin receptors on cultured and freshly isolated lymphocytes from normal subjects. Biochem. Biophys. Res. Commun. 163:1989;642-648.
4. Chomczynski P, Sacchi N Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:1987;156-159.
5. Gavériaux C, Peluso J, Simonin F, Laforet J, Kieffer B Identification of κ- and δ-opioid receptor transcripts in immune cells. FEBS Lett. 369:1995;272-276.
6. Gilmore W, Weiner LP The opioid specificity of beta-endorphin enhancement of murine lymphocyte proliferation. Immunopharmacology. 17:1989;19-30.
7. Gough N Rapid and quantitative preparation of cytoplasmic RNA from small numbers of cells. Anal. Biochem. 173:1988;93-95.
8. Guiramand J, Montmayeur J-P, Ceraline J, Bhatia M, Borrelli E Alternative splicing of the dopamine D2 receptor directs specificity of coupling to G-proteins. J. Biol. Chem. 270:1995;7354-7358.
9. Hasegawa H, Negishi M, Ichikawa A Two isoforms of the prostaglandin E receptor EP3 subtype different in agonist-independent constituve activity. J. Biol. Chem. 271:1996;1857-1860.
10. Kieffer BL, Befort K, Gaveriaux RC, Hirth CG The δ-opioid receptor: isolation of a cDNA by expression cloning and pharmacological characterization. Proc. Natl. Acad. Sci. USA. 89:1992;12048-12052.
11. Kieffer B Recent advances in molecular recognition and signal transduction of active peptides: receptors for opioid peptides. Cell. Mol. Neurobiol. 15:1995;615-635.
12. A.I. Lamond, (Ed.), Molecular Biology Intellingence Unit, Pre-mRNA processing, Springer, Berlin, Germany, 1995, 217 pp.
13. Makman MH, Bilfinger TV, Stefano GB Human granulocytes contain an opiate alkaloid-selective receptor mediating inhibition of cytokine-induced activation and chemotaxis. J. Immunol. 154:1995;1323-1330.
14. 3-opiate receptors. Eur. J. Pharmacol. 273:1995;R5-R6.
15. Mansson E, Bare L, Yang DM Isolation of a human κ opioid receptor cDNA from placenta. Biochem. Biophys. Res. Commun. 202:1994;1431-1437.
16. Mount SM A catalogue of splice junction sequences. Nucleic Acids Res. 10:1982;459-472.
17. Pasternak GW Pharmacological mechanisms of opioid analgesics. Clin. Pharmacol. 16:1993;1-18.
18. Schweigerer L, Schmidt W, Teschemacher H, Wilhelm S β-Endorphin: interaction with specific nonopioid binding sites on EL4 thymoma. Neuropeptides. 6:1985;445-452.
19. Shahabi NA, Peterson PK, Sharp B β-Endorphin binding to naloxone-insensitive sites on a human mononuclear cell line (U937): effects of cations and guanosine triphosphate. Endocrinology. 126:1990;3006-3015.
20. Shyamala V, Moulthrop HM, Stratton-Thomas J, Tekamp-Olson P Two distinct human endothelin B receptors generated by alternative splicing from a single gene. Cell. Mol. Biol. Res. 40:1994;285-296.
21. Simonin F, Befort K, Gaveriaux-Ruff Cet al. The human delta opioid receptor: genomic organization, cDNA cloning, functional expression and distribution in human brain. Mol. Pharmacol. 46:1994;1015-1021.
22. Simonin F, Gavériaux-Ruff C, Befort Ket al. Kappa opioid receptor in humans: cDNA and genomic cloning, chromosomal assignment, functional expression, pharmacology and expression pattern in the central nervous system. Proc. Natl. Acad. Sci. USA. 92:1995;7006-7010.
23. Snyder LA, Roberts JL, Sealfon SC Alternative transcripts of the rat and human dopamine D3 receptor. Biochem. Biophys. Res. Commun. 180:1991;1031-1035.
24. Spengler D, Waeber C, Pantaloni Cet al. Differential signal transduction variants of the PACAP receptor. Nature. 365:1993;170-175.
25. 125I]-β-endorphin binding sites. Life Sci. 56:1995;97-102.
26. Traynor J Subtypes of the κ-opioid receptor: fact or fiction? Trends Pharmacol. Sci. 10:1989;52-53.
27. Traynor JR, Elliot J δ-Opioid receptor subtypes and cross talk with μ-receptors. Trends Pharmacol. Sci. 14:1993;84-85.
28. Westphal M, Li CH β-Endorphin: demonstration of binding sites in three human neuroblastoma cell lines specific for the COOH-terminal segment of the human hormone. Biochem. Biophys. Res. Commun. 120:1984;873-878.
29. Zhu J, Chen C, Xue J-C, Kunapuli S, DeRiel JK, Liu-Chen L-Y Cloning of a human κ opioid receptor from the brain. Life Sci. 56:1995;201-207.
30. Zimprich A, Simon T, Höllt V Cloning and expression of an isoform of the rat mu opioid receptor (rMOR1B) which differs in agonist-induced desensitization from rMOR1. FEBS Lett. 359:1995;142-146.