Enhancement of immunohistochemical detection of HIV-1 p24 antigen in brain by tyramide signal amplification

Journal of Virological Methods

Volumn 67, Issue 1, 1997, Pages 103-112

  Strappe, Pádraig M ^a   Wang, Ting Huei ^a   McKenzie, Chris Anne ^a   Lowrie, Suzanne ^a   Simmonds, Peter ^a   Bell, Jeanne E ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Brain tissue; Human immunodeficiency virus type 1; Immunohistochemistry; p24 antigen; Tyramide signal amplification

Indexed keywords

ANTIGEN P24;

ARTICLE; AUTOPSY; BRAIN BIOPSY; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; HUMAN TISSUE; IMAGE ENHANCEMENT; IMMUNOFLUORESCENCE TEST; IMMUNOHISTOCHEMISTRY; MICROWAVE IRRADIATION; NONHUMAN; PRIORITY JOURNAL; VIRUS DETECTION; VIRUS MENINGITIS;

BRAIN; FLUORESCENT ANTIBODY TECHNIQUE; HIV ANTIBODIES; HIV CORE PROTEIN P24; HIV INFECTIONS; HIV-1; HUMANS; IMMUNOENZYME TECHNIQUES; MICROWAVES; PARAFFIN EMBEDDING; SENSITIVITY AND SPECIFICITY; TYRAMINE;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 0342601895     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(97)00083-9     Document Type: Article

References (21)

1. Achim, C.L., Wang, R., Miners, D.K., Wiley, C.A., 1994. Brain viral burden in HIV infection. J Neuropathol. Exp. Neurol. 53, 284-294.
2. Adams, J.C., 1992. Biotin amplification of biotin and horse raddish peroxidase signals in histochemical stains. J. Histochem. Cytochem. 40, 1457.
3. Bagasra, O., Lavi, E., Bobroski, L., Khalli, K., Pestaner, J., Tawadros, R., Pomerantz, R., 1996. Cellular resevoirs of HIV-1 in the central nervous system of infected individuals: identification by the combination of in situ polymerase chain reaction and immunohistochemistry. AIDS 10, 573-585.
4. Berghorn, K.A., Bonnett, J.H., Hoffman, G.E., 1994. c-Fos immunoreactivity is enhanced with biotin amplification. J. Histochem. Cytochem. 42, 1635-1642.
5. Bedetti, C.D., 1985. Immunocytochemical demonstration of cytochrome c oxidase with an immunoperoxidase method. J. Histochem. Cytochem. 33, 446-452.
6. Bobrow, M.N., Thomas, D.H., Shaughnessy, K.J., Litt, G.J., 1989. Catalyzed reporter deposition, a novel method of signal amplification. J. Immunol. Methods 125, 279-285.
7. Budka, H., 1991. Neuropathology of human immunodeficiency virus infection. Brain Pathol. 1, 163-175.
8. Cuevas, E.C., Bateman, A.C., Wilkins, B.S., Johnson, P.A., Williams, J.H., Lee, A.H.S., Jones, D.B., Wright, D.H., 1994. Microwave antigen retrieval in immunocytochemistry: a study of 80 antibodies. J. Clin. Pathol. 47, 448-452.
9. Davis, L.E., Hjelle, B.L., Miller, V.E., Palmer, D.L., Llewellyn, A.L., Merlin, T.L., Young, S.A., Mills, R.G., Wachsman, W., Wiley, C.A., 1992. Early viral brain invasion in iatrogenic human immunodeficiency virus infection. Neurology 42, 1736-1739.
10. Dondaldson, Y.K., Bell, J.E., Ironside, J.W., et al., 1994a. Redistribution of HIV outside the lymphoid system with onset of AIDS. Lancet 343, 343-382.
11. Dondaldson, Y.K., Bell, J.E., Holmes, E.C., Hughes, E.S., Brown, H.K., Simmonds, P., 1994b. In vivo distribution and cytopathology of variants of human immunodeficiency virus type 1 showing restricted sequence variability in the V3 loop. J. Virol. 68, 5991-6005.
12. Esiri, M.M., Morris, C.S., Millard, P.R., 1991. Fate of oligodendrocytes in HIV-1 infection. AIDS 5, 1081-1088.
13. Falagola, M.F., Hanly, A., Galvao-Castro, B., Petito, C.K., 1995. HIV infection of Human choroid plexus: a possible mechanism of viral entry into the CNS. J Neuropathol. Exp. Neurol. 54, 497-503.
14. Kerstens, H.M.J., Poddighe, P.J., Hanselarr, A.G.J.M., 1995. A novel in situ hybridization signal amplification method based on the deposition of biotinylated tyramine. J. Histochem. Cytochem. 43, 347-352.
15. McQuaid, S., McConnell, R., McMahon, J., Herron, B., 1995. Microwave antigen retrieval for immunocytochemistry on formalin-fixed, paraffin-embedded post-mortem CNS tissue. J. Pathol. 176, 207-216.
16. Nuovo, G., Gallery, F., MacConell, P., Braun, A., 1994. In situ detection of polymerase chain reaction-amplified HIV-1 nucleic acids and tumour necrosis factor alpha RNA in the central nervous system. Am. J. Pathol. 144, 659-666.
17. Price, R.W., Understanding the AIDS dementia complex (ADC). In: Price R.W., Perry S.W. HIV, AIDS and the Brain. Raven Press, New York, 1994, pp. 1-45.
18. Ranki, A., Nyberg, M., Vladimar, Ovod, Haltia, M., Elovaar, I., Raininko, R., Haapasalo, H., Krohn, K., 1995. Abundant expression of HIV nef and rev proteins in brain astrocytes in vivo is associated with dementia. AIDS 9, 1001-1008.
19. Saito, Y., Sharer, L.R., Epstein, L.G., Michaels, J., Mintz, M., Louder, M., Golding, K., Cvetkovich, T.A., Blumberg, B.M., 1994. Overexpression of nef as a marker for restricted HIV-1 infection of astrocytes in post-mortem pediatric central nervous tissues. Neurology 44, 474-481.
20. Tonatore, C., Chandra, R., Berger, J.R., Major, E.O., 1994. HIV-1 infection of subcortical astrocytes in the pediatric central nervous system. Neurology 44, 481-487.
21. Wiley, C.A., Schrier, R.D., Nelson, J.A., Lampert, P.W., Oldstone, M.B.A., 1986. Cellular localisation of human immunodeficiency virus infection within the brains of acquired immunodeficiency syndrome patients. Proc. Natl. Acad. Sci. USA 83, 7089-7093.