Determination of the apparent molecular weight cut-off for the fluorimetric calcofluor-FIA method when detecting (1 → 3),(1 → 4)-β-D-glucan using a high ionic strength eluant

Journal of Cereal Science

Volumn 31, Issue 2, 2000, Pages 155-157

  Gomez C ^a   Navarro, A ^a   Carbonell, J V ^b   Sendra, J M ^b  

^a Asoc de Invest de Cerveza y Malta   (Spain)

^b INSTITUTO DE AGROQUÍMICA Y TECNOLOGÍA DE ALIMENTOS CSIC   (Spain)

Author keywords

(1 3),(1 4) D glucan; Calcofluor; FIA; Molecular weight cut off

Indexed keywords

EID: 0342572573     PISSN: 07335210     EISSN: None     Source Type: Journal    
DOI: 10.1006/jcrs.1999.0247     Document Type: Article

References (11)

1. McCleary B.V., Nurthen E. Measurement of (1→3),(1→4)-β-D-glucan in malt, wort and beer. Journal of the Institute of Brewing. 92:1986;168-173.
2. Wood P.J., Weisz J. Use of Calcofluor in analysis of oat β-glucan. Cereal Chemistry. 61:1984;73-75.
3. Jørgensen K.G., Jensen S.A., Hartlev P., Munck L. The analysis of β-glucan in wort and beer using Calcofluor. European Brewery Convention, Proceedings of the 20th Congress, Helsinki. 1985;403-410.
4. Mekis E., Pinter G., Bendek G. Modified fluorimetric flow-injection-analysis (FIA) method for the determination of (1→3),(1→4)-β-D-glucan. Journal of the Institute of Brewing. 93:1987;396-398.
5. Sendra J.M., Carbonell J.V., Gosalbes M.J., Todo V. Determination of β-glucan in wort and beer by its binding with Calcofluor, using a fluorimetric flow- injection-analysis (FIA) method. Journal of the Institute of Brewing. 95:1989;327-332.
6. Wood P.J. Specificity in the interaction of direct dyes with polysaccharides. Carbohydrate Research. 85:1980;271-287.
7. Manzanares P., Navarro A., Sendra J.M., Carbonell J.V. Selective determination of β-glucan of differing molecular size, using the fluorimetric Flow-Injection-Analysis (FIA) method. Journal of the Institute of Brewing. 97:1991;101-104.
8. Manzanares P., Navarro A., Sendra J.M., Carbonell J.V. Determination of the average molecular weight of barley β-glucan within the range 30-100k by the Calcofluor-FIA method. Journal of Cereal Science. 17:1993;211-223.
9. Navarro A., Manzanares P., Carbonell J.V., Sendra J.M. Determination of (1→3),(1→4)-β-D-glucanase activity by a Calcofluor-flow injection analysis method. Journal of Cereal Science. 22:1995;275-284.
10. Sendra J.M., Carbonell J.V. A theoretical equation describing the time evolution of the concentration of a selected range of substrate molecular weights in depolymerization processes mediated by single-attack mechanism endo -enzymes. Biotechnology and Bioengineering. 57:1998;387-393.
11. Gómez C., Navarro A., Manzanares P., Horta A., Carbonell J.V. Physical and structural properties of barley (1→3),(1→4)-β-D-glucan. I. Determination of molecular weight and macromolecular radius by light scattering. Carbohydrate Polymers. 32:1997;7-15.