Stability studies of a recombinant cutinase immobilized to dextran and derivatized silica supports

Enzyme and Microbial Technology

Volumn 24, Issue 1-2, 1999, Pages 60-66

  Goncalves A M ^a   Schacht, E ^c   Matthijs, G ^c   Aires Barros, M R ^b   Cabral, J M S ^b   Gil, M H ^a  

^a UNIVERSITY OF COIMBRA   (Portugal)

^b INSTITUTO SUPERIOR TÉCNICO   (Portugal)

^c GHENT UNIVERSITY   (Belgium)

Author keywords

Conformational stability; Cutinase; Dextran; Immobilization; Thermostability

Indexed keywords

CATALYST SUPPORTS; CONFORMATIONS; DERIVATIVES; DIFFERENTIAL SCANNING CALORIMETRY; ENZYME KINETICS; POLYSACCHARIDES; SILICA; SOLUBILITY; THERMODYNAMIC STABILITY;

CONFORMATIONAL STABILITY; CUTINASE; DEXTRAN;

ENZYME IMMOBILIZATION;

CUTINASE; DEXTRAN; HYDROXIDE; RECOMBINANT ENZYME; SILICON DIOXIDE;

ARTICLE; CULTURE MEDIUM; DIFFERENTIAL SCANNING CALORIMETRY; ENZYME CONFORMATION; ENZYME IMMOBILIZATION; ENZYME KINETICS; ENZYME STABILITY; FUSARIUM SOLANI; IMMOBILIZATION; NONHUMAN;

FUSARIUM SOLANI;

EID: 0342459772     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(98)00089-1     Document Type: Article

References (36)

1. Mozhaev V. Mechanism-based strategies for protein thermostabilization. Tibtech. 11:1993;88-95.
2. Clark D.S. Can immobilisation be exploited to modify enzyme activity? Tibtech. 12:1994;439-443.
3. Klibanov A.M. Enzyme stabilisation by immobilisation. Anal. Biochem. 93:1979;1-25.
4. Martinek K., Klibanov A.M., Goldmacher V.S., Berezin I.V.I. Increase in thermostability of enzymes covalently bound to a complementary surface of a polymer support in a multipoint fashion. Biochim. Biophys. Acta. 485:1977;1-12.
5. Guísan J.M. Aldehyde-agarose gels as activated supports for the immobilisation-stabilisation of enzymes. Enzyme Microb. Technol. 10:1988;375-382.
6. Guísan J.M., Bastida A., Cuesta C., Fernandez-Lafuente R., Rosell C.M. Immobilisation-stabilisation of α-chymotrypsin by covalent attachment to aldehyde-agarose gels. Biotechnol. Bioeng. 38:1991;1144-1152.
7. Mozhaev V., Sergeeva A.B., Khmelnitsky Y. Multipoint attachment to a support protects enzyme from inactivation by organic solvents α-chymotrypsin in aqueous solutions of alcohols and diols . Biotechnol. Bioeng. 35:1990;653-659.
8. Goldstein L. Microenvironmental effects on enzyme catalysis. A kinetic study of polyanionic and polycationic derivatives of chymotrypsin. Biochemistry. 11:1972;4072-4084.
9. Martinez C., De Geuss P., Lauwereys M., Matthyssens G., Cambillau C. Fusarium solani cutinase is a lipolytic enzyme with a catalytic serine accessible to solvent. Nature. 356:1992;615-618.
10. Martinez C., Nicolas A., von Tilbeurgh H., Egloff M.F., Cudrey C., Verger R., Cambillau C. Cutinase, a lipolytic enzyme with a preformed oxyanion hole. Biochemistry. 33:1994;83-89.
11. Gonçalves A.P.V., Lopes J.M., Lemos F., Ramoa Ribeiro F., Prazeres D.M.F., Cabral J.M.S., Aires-Barros M.R. Zeolites as supports for enzymatic hydrolysis reactions. Comparative study of several zeolites. J. Mol. Catalysis B: Enzymatic. 1:1996;53-60.
12. Sebastiao M.J., Cabral J.M.S., Aires-Barros M.R. Synthesis of fatty acid esters by a recombinant cutinase in reversed micelles. Biotechnol. Bioeng. 42:1993;326-332.
13. Melo E.P., Aires-Barros M.R., Cabral J.M.S. Triglyceride hydrolysis and stability of recombinant cutinase from Fusarium solani in AOT-isooctane-reversed micelles. Appl. Biochem. Biotechnol. 50:1995;45-56.
14. Cunnah P.J., Aires-Barros M.R., Cabral J.M.S. Esterification and transesterification catalysed by cutinase in reversed micelles of CTAB for the synthesis of short chain esters. Biocatal. Biotransform. 14:1996;125-146.
15. Privalov P., Gill S.J. Stability of protein structure and hydrophobic interaction. Adv. Prot. Chem. 39:1988;191-234.
16. Battistel E., Bianchi D. Influence of the solvent properties on protein stability in organic media. van den Tweel W., Buitelaar R.M. Stability and Stabilisation of Enzymes. 1993;13-20 Elsevier Science Publishers, New York.
17. Steadman B., Thompson K.C., Middlaugh C.R., Matsuno K., Vrona S., Lawson E., Lewis R. The effects of surface adsorption on the thermal stability of proteins. Biotechnol. Bioeng. 40:1992;8-15.
18. Back J., Oakenfull D., Smith M. Increased thermal stability of proteins in the presence of sugars and polyols. Biochemistry. 18:(23):1979;5191-5196.
19. Otamiri M., Adlercreutz P., Mattiasson B. A differential scanning calorimetric study of chymotrypsin in the presence of added polymers. Biotechnol. Bioeng. 44:1994;73-78.
20. Volkin D., Staubli A., Langer R., Klibanov A.M. Enzyme thermoinactivation in anhydrous organic solvents. Biotechnol. Bioeng. 37:1991;843-853.
21. Barone G., del Vecchio P., Fessas D., Giancola C., Graziano G., Riccio A. Protein stability in non-aqueous media A DSC study . van den Tweel W., Buitelaar R.M. Stability and Stabilisation of Enzymes. 1993;37-44 Elsevier Science Publishers, New York.
22. Matthijs G., Schacht E. Dextran coated silica and its behaviour in high performance size exclusion chromatography. J. Chromatogr. A. 755:1996;1-10.
23. Lowry R.R., Rosenbrough N.J., Farr L., Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:1951;265-275.
24. Jackson R.L., Shirai K. Lipoprotein lipase-catalyzed hydiolysis of p-nitrophenyl butyrate. J. Biol. Chem. 257:1982;1253-1258.
25. Winkler U.K., Stuckmann M. Glycogen, hyaluronate, and some other polysaccharides greatly enhance the formation of exolipase by Serratia marcescens. J. Bacteriol. 138:1979;663-670.
26. Combes D., Monsan P. Effects of polyhydric alcohols on invertase stabilisation. Ann. N. Y. Acad. Sci. 434:1984;61-63.
27. Monsan P., Combes D. Effect of water activity on enzyme activity and stability. Ann. N. Y. Acad. Sci. 542:1988;48-60.
28. Gray C.J. Additives and enzyme stability. Biocatalysis. 1:1988;187-196.
29. Gibson T., Hulbert J.N., Pierce B., Webster J.I. The stabilisation of analytical enzymes using polyelectrolytes and sugar derivatives. van den Tweel W., Buitelaar R.M. Stability and Stabilisation of Enzymes. 1993;337-346 Elsevier Science Publishers.
30. Hendrickx M., DeCordt S., Maesmans G., Tobback P. The influence of polyalcohols and carbohydrates on the thermostability of α-amylase. Biotechnol. Bioeng. 43:1994;107-114.
31. Wong S.S., Wong L.-J. Chemical cross-linking and the stabilisation of proteins and enzymes. Enzyme Microb. Technol. 14:1992;866-874.
32. Blanco R., Guísan J.M. Stabilisation of enzymes by multi-point covalent attachment to agarose-aldehyde gels. Borohydride reduction of trypsin-agarose derivatives. Enzyme Microb. Technol. 11:1989;360-366.
33. Bosley J.A., Clayton J.C. Blueprint for a lipase support Use of hydrophobic controlled-pore glasses as model systems . Biotechnol. Bioeng. 43:1994;934-938.
34. Sadana A. Biocatalysis Fundamentals in Enzyme Deactivation Kinetics . 1991;32-67 Prentice Hall, Englewood Cliffs, NJ.
35. Henley J.P., Sadana A. Categorization of enzyme deactivations using a series-type mechanism. Enzyme Microb. Technol. 7:1985;50-60.
36. Matthys, G. Ph.D. thesis, University of Ghent, Ghent, Belgium, 1997.