Cellulose binding domains and linker sequences potentiate the activity of hemicellulases against complex substrates

Journal of Biotechnology

Volumn 57, Issue 1-3, 1997, Pages 59-69

  Black, Gary W ^a,c   Rixon, Jane E ^a   Clarke, Jonathan H ^b   Hazlewood, Geoffrey P ^b   Ferreira, Luis M A ^a,d   Bolam, David N ^a   Gilbert, Harry J ^a  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

^b BABRAHAM INSTITUTE   (United Kingdom)

^c UNIVERSITY OF SUNDERLAND   (United Kingdom)

^d Pólo Universitário do Alto da Ajuda   (Portugal)

Author keywords

Cellulose binding domains; Hemicellulases; Linker sequences

Indexed keywords

CATALYST ACTIVITY; CELLS; CELLULOSE; PLANTS (BOTANY); SUBSTRATES;

CELLULOSE BINDING DOMAIN; HEMICELLULASES; LINKER SEQUENCES;

ENZYMES;

BACTERIAL ENZYME; CELLULOSE; HEMICELLULASE; HEMICELLULOSE; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; BIODEGRADATION; CELL WALL; CONTROLLED STUDY; ENZYME ACTIVITY; NONHUMAN; PLANT CELL; PRIORITY JOURNAL; PSEUDOMONAS;

BINDING SITES; CELLULOSE; ENDO-1,4-BETA XYLANASES; GLYCOSIDE HYDROLASES; POLYSACCHARIDES; PSEUDOMONAS; XYLOSIDASES;

BACTERIA (MICROORGANISMS); PSEUDOMONAS;

EID: 0342437551     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(97)00089-8     Document Type: Article

References (33)

1. Black, G.W., Hazlewood, G.P., Xue, G.P., Orpin, C.G., Gilbert, H.J., 1994. Xylanase B from Neocallimastix patriciarum contains a non-catalytic 455-residue linker sequence comprised of 57 repeats of an octapeptide. Biochem. J. 299, 381-387.
2. Chambers, S.P., Prior, S.E., Barstow, D.A., Minton, N.P., 1988. The pMTL nic-cloning vectors. 1. Improved pUC polylinker regions to facilitate the use of sonicated DNA for nucleotide sequencing. Gene 68, 139-149.
3. Clarke, J.H., Laune, J.I., Gilbert, H.J., Hazlewood, G.P., 1991. Multiple xylanases of Cellulomonas fimi are encoded by distinct genes. FEMS Microbiol. Lett. 83, 305-310.
4. Clarke, J.H., Davidson, K., Gilbert, H.J., Fontes, C.M.G.A., Hazlewood, G.P., 1996. A modular xylanase from mesophilic Cellulomonas fimi contains the same cellulose-binding and thermostabilizing domains as xylanases from thermophilic bacteria. FEMS Microbiol. Lett. 139, 27-35.
5. Coutinho, J.B., Gilkes, N.R., Warren, R.A.J., Kilburn, D.G., Miller, R.C. Jr., 1993. The nature of the cellulose-binding domain affects the activities of a bacterial endoglucanase on different forms of cellulose. FEMS Microbiol. Lett. 113, 211-218.
6. Din, N., Gilkes, N.R., Tekant, B., Miller Jr, R.C., Warren, R.A.J., Kilburn, D.G., 1991. Non-hydrolytic disruption of cellulose fibres by the binding domain of a bacterial cellulase. Bio/Technol. 9, 1096-1099.
7. Din, N., Damude, H.G., Gilkes, N.R., Miller, R.C., Warren, R.A.J., Kilburn, D.G., 1994. C-1-C-X revisited-Intramolecular synergism in a cellulase. Proc. Natl. Acad. Sci. USA 91, 11383-11387.
8. Fanutti, C., Ponyi, T., Black, G.W., Hazlewood, G.P., Gilbert, H.J., 1995. The conserved non-catalytic 40-residue sequence in cellulases and hemicellulases from anaerobic fungi functions as a protein docking domain. J. Biol. Chem. 270, 29314-29322.
9. Ferriera, L.M.A., Durrant, A.J., Hall, J., Hazlewood, G.P., Gilbert, H.J., 1990. Spatial separation of protein domains is not necessary for catalytic activity or substrate binding in a xylanase. Biochem. J. 269, 261-264.
10. Ferreira, L.M.A., Wood, T.M., Williamson, G., Faulds, C., Hazlewood, G.P., Black, G.W., Gilbert, H.J., 1993. A modular esterase from Pseudomonas fluorescent subsp. cellulosa contains a non-catalytic cellulose-binding domain. Biochem J. 294, 349-355.
11. Fontes, C.M.G.A., Hazlewood, G.P., Morag, E., Hall, J., Hirst, B.H., Gilbert, H.J., 1995. Evidence for a general role for non-catalytic thermostabilizing domains in xylanases from thermophilic bacteria. Biochem. J. 307, 151-158.
12. Ghangas, G.S., Wilson, D.B., 1988. Cloning of the Thermomonospora fusca xylanase gene and its expression in Escherichia coli and Streptomyces lividans. J. Bacteriol. 171, 2963-2969.
13. Gilbert, H.J., Blazek, R., Bullman, H.M.S., Minton, N.P., 1986. Cloning and expression of the Erwinia chrysanthemi asparaginase gene in Escherichia coli and Erwinia carotovora. J. Gen. Microbiol. 132, 151-160.
14. Gilbert, H.J., Sullivan, D.A., Jenkins, G., Kellett, L.E., Minton, N.P., Hall, J., 1988. Molecular cloning of multiple xylanase genes from Pseudomonas fluorescens subsp. cellulosa. J. Gen. Microbiol. 134, 3239-3247.
15. Gilbert, H.J., Hazlewood, G.P., Laurie, J.I., Orpin, C.G., Xue, G.P., 1992. Homologous catalytic domains in a rumen fungal xylanase: evidence for gene duplication and prokaryotic origin. Mol. Microbiol. 6, 2065-2072.
16. Gilbert, H.J., Hazlewood, G.P., 1993. Bacterial cellulases and xylanases. J. Gen. Microbiol. 139, 187-194.
17. Hall, J., Hazlewood, G.P., Huskisson, N.S., Durrant, A.J., Gilbert, H.J., 1989. Conserved serine-rich sequences in xylanase and cellulase from Pseudomonas fluorescens subspecies cellulosa: internal signal sequence and unusual protein processing. Mol. Microbiol. 3, 1211-1219.
18. Hall, J., Black, G.W., Ferrira, L.M.A., Millward-Sadler, S.J., Ali, B.R.S., Hazlewood, G.P., Gilben, H.J., 1995. The non-catalytic cellulose-binding domain of a novel cellulase from Pseudomonas fluorescens subsp. cellulosa is important for the efficient hydrolysis of Avicel. Biochem. J. 309, 749-756.
19. Harris, G.W., Jenkins, J.A., Connerton, I., Cummings, N., Lo Leggio, L., Scott, M., Hazlewood, G.P., Laurie, J.L., Gilbert, H.J., Pickersgill, R.W., 1994. Structure of the catalytic core of the family F xylanase from Pseudomonas fluorescens and identification of the xylopentaose-binding sites. Structure 2, 1107-1116.
20. John, M., Rohrig, H., Schmidt, J., Wieneke, U., Schell, J., 1993. Rhizobium NodB protein involved in nodulation signal synthesis is a chitooligosacchride deacetylase. Proc. Natl. Acad. Sci. USA 90, 625-629.
21. Kellett, L.E., Poole, D.M., Ferreira, L.M.A., Durrant, A.J., Hazlewood, G.P., Gilbert, H.J., 1990. Xylanase B and an arabinofuranosidase from Pseudomonas fluorescens subsp. cellulosa contain identical cellulose-binding domains and are encoded by adjacent genes. Biochem. J. 272, 369-376.
22. Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
23. McQueen-Mason, S.J., 1995. Expansins and cell wall expansion. J. Exp. Bot. 46, 1639-1656.
24. Miller, G.L., 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal. Chem. 31, 426-428.
25. Millward-Sadler, S.J., Poole, D.M., Henrissat, B., Hazlewood, G.P., Clarke, J.H., Gilbert, H.J., 1994. Evidence for a general role for high-affinity non-catalytic cellulose binding domains in microbial plant cell wall hydrolases. Mol. Microbiol. 11, 375-382.
26. Millward-Sadler, S.J., Davidson, K., Hazlewood, G.P., Black, G.W., Gilbert, H.J., Clarke, J.H., 1995. Novel cellulose-binding domains, NodB homologues and conserved modular architecture in xylanases from the aerobic soil bacteria Pseudomonas fluorescens subsp. cellulosa and Cellvibrio mixtus. Biochem. J. 312, 39-48.
27. Norrander, J., Kemp, T., Messing, J., 1983. Construction of improved M13-vectors using oligodeoxynucleotide-directed mutagenesis. Gene 26, 101-106.
28. Sedmak, J.J., Grossberg, J.R., 1977. A rapid, sensitive and versatile assay for protein using Coomassie Brilliant Blue G250. Anal. Biochem. 79, 544-552.
29. Shen, H., Schmuck, M., Pilz, I., Gilkes, N.R., Kilburn, D.G., Miller, R.C. Jr., 1991. Deletion of the linker connecting the catalytic and cellulose binding domains of endoglucanase-A (CENA) of Cellulomonas fimi alters its conformation and catalytic activity. J. Biol. Chem. 266, 11335-11340.
30. Soole, K.L., Hall, J., Jepson, M.A., Hazlewood, G.P., Hirst, B.H., Gilbert, H.J., 1992. Constitutive secretion of a bacterial enzyme by polarized epithelial cells. J. Cell Sci. 102, 495-504.
31. Srisodsuk, M., Reinikainen, T., Penttila, M., Teeri, T.T., 1993. Role of the interdomain linker peptide of Trichoderma reesei cellobiohydrolase-I in its interaction with crystalline cellulose. J. Biol. Chem. 268, 20756-20761.
32. Tomme, P., van Tilbeurgh, H., Pettersson, G., van Damme, J., Vanderckhove, J., Knowles, J., Teeri, T.T., Claeyssens, M., 1988. Studies of the cellulolytic system of Trichoderma reesei QM-9414 - analysis of domain function in 2 cellobiohydrolases by limited proteolysis. Eur. J. Biochem. 170, 575-581.
33. Tomme, P., Warren, R.A.J., Gilkes, N.R., 1995. Celluose hydrolysis by bacteria and fungi. Adv. Microb. Physiol. 37, 1-81.