N-acetylcysteine protects against age-related increase in oxidized proteins in mouse synaptic mitochondria

Brain Research

Volumn 762, Issue 1-2, 1997, Pages 256-258

  Banaclocha, Marcos Martínez ^a   Hernández, Ana Isabel ^b   Martínez, Natividad ^c   Ferrándiz, María Luisa ^d  

^a HOSPITAL UNIVERSITARIO LA PAZ   (Spain)

^b UNIVERSITY OF ALICANTE   (Spain)

^c HOSPITAL GENERAL UNIVERSITARIO DE ALICANTE   (Spain)

^d UNIVERSITY OF VALENCIA   (Spain)

Author keywords

Aging; Antioxidant; Protein carbonyl; Synaptic mitochondrion; Thiol

Indexed keywords

5,5' DITHIOBIS(2 NITROBENZOIC ACID); ACETYLCYSTEINE; ANTIOXIDANT; BRAIN PROTEIN; CARBONYL DERIVATIVE; GLUTATHIONE;

AEROBIC METABOLISM; AGED; AGING; ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIOXIDANT ACTIVITY; ARTICLE; BRAIN METABOLISM; BRAIN MITOCHONDRION; CONTROLLED STUDY; FEMALE; MOUSE; NERVE DEGENERATION; NONHUMAN; PARKINSON DISEASE; PRIORITY JOURNAL; SYNAPSE; SYNAPTOSOME;

ACETYLCYSTEINE; AGING; ANIMALS; FEMALE; FREE RADICAL SCAVENGERS; GLUTATHIONE; MICE; MICE, INBRED STRAINS; MITOCHONDRIA; NEURONS; OXIDATION-REDUCTION; SULFHYDRYL COMPOUNDS; SYNAPSES;

EID: 0342378119     PISSN: 00068993     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-8993(97)00493-9     Document Type: Article

References (24)

1. Aruoma O.I., Halliwell B., Hoey B.M., Butler J. The antioxidant action of N-acetylcysteine: its reaction with hydrogen peroxide, hydroxyl radical, superoxide, and hypochlorous acid. Free Rad. Biol. Med. 6:1989;593-597.
2. Beal M.F. Mitochondria, free radicals, and neurodegeneration. Curr. Opion. Neurobiol. 6:1996;661-666.
3. C.A. Colton, F. Pagan, J. Snell, J.S. Colton, A. Cummins, and D.L. Gilbert, Protection from oxidation enhances the survival of cultured mesencephalic neurons, Exp. Neurol. 132 (1995) 54-61.
4. De Flora S., Izzotti A., D'Agostini F., Cesarone C.F. Antioxidant activity and other mechanisms of thiols involved in chemoprevention of mutation and cancer. Am. J. Med. 91:1991;122-130.
5. Ellis E.F., Dodson L.Y., Police R.J. Restoration of cerebrovascular responsiveness to hyperventilation by the oxygen radical scavenger n-acetylcysteine following experimental traumatic brain injury. J. Neurosurg. 75:1991;774-779.
6. Haugaard N., Lee N.H., Kostrzewa R., Horn R.S., Haugaard E.S. The role of sulfhydryl groups in oxidative phosphorylation and ion transport by rat liver mitochondria. Biochem. Biophys. Acta. 172:1969;198-204.
7. Holmgren A. Thioredoxin and glutaredoxin systems. J. Biol. Chem. 264:1989;13963-13966.
8. N.S. Kosower, E.M. Kosower, Functions of glutathione: biochemical, physiological, toxicological and clinical aspects, in: A. Larsson, S. Orrenius, A. Holmgren, B. Mannervik (Eds.), Functions of Glutathione, Raven, New York, NY, 1983, pp. 307-315.
9. Lai J.C.K., Clark J.B. Preparation and properties of mitochondria derived from synaptosomes. Biochem. J. 154:1976;421-432.
10. Lowry O.H., Rosenbrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:1951;265-275.
11. Martínez M., Ferrándiz M.L., De Juan E., Miquel J. Age related changes in glutathione and lipid peroxide content in mouse synaptic mitochondria: relationship to cytochrome c oxidase decline. Neurosci. Lett. 170:1994;121-124.
12. Martínez M., Ferrándiz M.L., Díez A., Miquel J. Depletion of cytosolic GSH decreases the ATP levels and viability of synaptosomes from aged mice but not from young mice. Mech. Ageing Dev. 84:1995;77-81.
13. Martínez M., Hernández A.I., Martínez N., Ferrándiz M.L. Age-related increase in oxidized proteins in mouse synaptic mitochondria. Brain Res. 731:1996;246-248.
14. Miquel J., Ferrándiz M.L., De Juan E., Sevila I., Martínez M. N-Acetylcysteine protects against age-related decline of oxidative phosphorylation in liver mitochondria. Eur. J. Pharmacol. 292:1995;333-335.
15. J. Miquel and H. Weber, Aging and increased oxidation of the sulfur pool. In: J. Viña (Ed.), Glutathione: Metabolism and Physiological Functions, CRC, Boca Raton, FL, 1990, pp. 187-197.
16. Nicholls D.G. Calcium transport and proton electrochemical potential gradient in mitochondria from guinea-pig cerebral cortex and rat heart. Biochem. J. 170:1978;511-522.
17. Oliver C.N., Ahn B.-W., Moerman E.J., Goldstein S., Stadtman E.R. Age-related changes in oxidized proteins. J. Biol. Chem. 262:1987;5488-5491.
18. Ravindranath V., Reed D.J. Glutathione depletion and formation of glutathione-protein mixed disulfide following exposure of brain mitochondria to oxidative stress. Biochem. Biophys. Res. Commun. 169:1990;1075-1079.
19. Sheffner A.L., Medler E.M., Bailey K.R., Gallo D.G., Mueller A.J.et al. Metabolic studies with acetylcysteine. Biochem. Pharmacol. 15:1966;1523-1535.
20. Siliprandi D., Siliprandi N., Scutari G., Zoccarato F. Restoration of some energy linked processes lost during the ageing of rat liver mitochondria. Biochem. Biophys. Res. Commun. 55:1973;563-567.
21. Smith C.D., Carney J.M., Starke-Reed P.E., Oliver C.N., Stadtman E.R., Floyd R.A., Markesbery W.R. Excess brain protein oxidation and enzyme dysfunction in normal aging and in Alzheimer's disease. Proc. Natl. Acad. Sci. USA. 88:1991;10540-10543.
22. Smith C.D., Carney J.M., Tatsumo T., Stadtman E.R., Floyd R.A., Markesbery W.R. Protein oxidation in aging brain. Ann. NY Acad. Sci. 663:1992;110-119.
23. Stadtman E.R. Protein oxidation and aging. Science. 257:1992;1220-1224.
24. Yagi T., Hatefi Y. Thiols in oxidative phosphorylation: inhibition and energy-potentiated uncoupling by monothiol and dithiol modifiers. Biochemistry. 23:1984;2449-2455.