Modeling retrovirus production for gene therapy. 2. Integrated optimization of bioreaction and downstream processing

Biotechnology Progress

Volumn 16, Issue 3, 2000, Pages 350-357

  Cruz, Pedro E ^a   Gonçalves, Délia ^a   Almeida, Jonas ^a   Moreira, José L ^a   Carrondo, Manuel J T ^a,b  

^a INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^b UNIVERSIDADE NOVA DE LISBOA   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

BIOREACTION KINETICS; DOWNSTREAM PROCESSING; GENETIC MARKER; LACZ GENE; PERFUSION; PROCESS MODEL; PROCESS OPTIMIZATION; RETROVIRUS; SENSITIVITY ANALYSIS; STIRRED REACTOR; ULTRAFILTRATION; VIRUS CULTURE; VIRUS PRODUCTION;

CELLS; GENETIC ENGINEERING; MATHEMATICAL MODELS; PURIFICATION; SENSITIVITY ANALYSIS; ULTRAFILTRATION;

BIOREACTION; GENE THERAPY; MOLONEY MURINE LEUKEMIA VIRUS;

VIRUSES;

BIOREACTORS; CELL LINE; CHROMATOGRAPHY, GEL; GENE THERAPY; RETROVIRIDAE; VIRUS ASSEMBLY; VIRUS REPLICATION;

MOLONEY MURINE LEUKEMIA VIRUS; MURINAE; UNIDENTIFIED RETROVIRUS;

EID: 0342368898     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp000017l     Document Type: Article

References (9)

1. Bowles, N. E.; Eisensmith, R. C.; Mohuiddin, R.; Pyron, M.; Woo, S. L. C. A Simple and Efficient Method for the Concentration and Purification of Recombinant Retrovirus for Increased Hepatocyte Transduction in Vivo. Hum. Gene Ther. 1996, 7, 1735-1742.
2. Bieganski, R. M.; Fowler, A.; Morgan, J. R.; Toner, M. Stabilization of Active Recombinant Retroviruses in an Amorphous Dry State with Trehalose. Biotechnol. Prog. 1998, 14, 615-6200.
3. Lee, S.-G.; Kim, S.; Robbins, P. D.; Kim, B.-G. Optimization of Environmental Factors for the Production and Handling of Recombinant Retroviruses. Appl. Microbiol. Biotechnol. 1996, 46, 477-483.
4. Saha, K.; Lin, Y. C.; Wong, P. K. A Simple Method for Obtaining a Highly Viable Virus from Culture Supernatant. J. Virol. Methods 1994, 46, 349-352.
5. Miller, D. L.; Meikle, P. J.; Anson, D. S. A Rapid and Efficient Method for Concentration of Small Volumes of Retroviral Supernatant. Nucleic Acids Res. 1996, 24, 1576-1577.
6. Vits, H.; Hu, W.-S. Fluctuations in Continuous Mammalian Cell Bioreactors with Retention. Biotechnol. Prog. 1992, 8, 397-403.
7. Hiller, G. W.; Clark, D. S.; Blanch, H. W. Cell Retention-Chemostat Studies of Hybridoma Cells-Analysis of Hybridoma Growth and Metabolism in Continuous Suspension Culture on Serum-Free Medium. Biotechnol. Bioeng. 1993, 42, 185-195.
8. Hermia, J. Constant Pressure Blocking Filtration Laws: Application to Power-Law Non-Newtonian Fluids. Trans. Inst. Chem. Eng. 1982, 60, 183-187.
9. Field, R. W.; Wu, D.; Howell, J. A.; Gupta, B. B. Critical flux concepts for microfiltration fouling. J. Membrane Sci. 1995, 100, 259-272.