Peptide condensation activity of a neutral protease from Vibrio sp. T1800 (Vimelysin)

Biotechnology and Bioengineering

Volumn 53, Issue 4, 1997, Pages 387-390

  Kunugi, Shigeru ^a   Koyasu, Akira ^a   Takahashi, Saori ^a   Oda, Kohei ^a  

^a KYOTO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

enzymatic synthesis; organic solvent; peptide synthesis; protease; thermolysin; Vibrio

Indexed keywords

BIOSYNTHESIS; CATALYST ACTIVITY; CONDENSATION; ENZYME KINETICS; ORGANIC SOLVENTS;

ENZYMATIC SYNTHESIS; NEUTRAL PROTEASES; PEPTIDE SYNTHESIS; THERMOLYSIN;

ENZYMES;

NEUTRAL PROTEINASE;

ARTICLE; BIOENGINEERING; ENZYME SYNTHESIS; NONHUMAN; PEPTIDE SYNTHESIS; POLYMERIZATION; VIBRIO;

EID: 0342275235     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19970220)53:4<387::AID-BIT5>3.0.CO;2-J     Document Type: Article

References (21)

1. Fruton, J. S. 1982. Proteinase-catalyzed synthesis of peptide bonds. Adv. Enzymol. 53: 239-300.
2. Homandberg, G. A., Mattis, J. A., Laskowski Jr, M. 1978. Synthesis of peptide bonds by proteinases. Addition of organic cosolvent shifts peptide bond equilibria toward synthesis. Biochemistry, 17: 5220-5227.
3. Isowa, Y., Ohmori, M., Ichikawa, T., Mori, K. 1979. The thermolysin-catalyzed condensation reactions of N-substituted aspartic and glutamic acids with phenylalanine alkyl esters. Tetrahedron Lett. 28: 2611-2612.
4. Keqin, C., Robinson, A. C., Van Dam, M. E., Martinez, P., Economou, C., Arnold, F. H. 1991. Enzyme engineering for nonaqueous solvents. II. Additive effects of mutations on the stability and activity of subtilisin E in polar organic media. Biotechnol. Prog. 7: 125-129.
5. Klibanov, A. M. 1986. Enzymes that work in organic solvent. Chemtech. 00: 354-359.
6. Kunugi, S., Hirohara, H., Ise, N. 1982. pH and temperature dependence of thermolysin catalysis. Eur. J. Biochem. 124: 157-163.
7. Kunugi, S., Suzuki, N., Sakamoto, A., Yoshida, M. 1995. Kinetics of the peptide formation catalyzed by thermolysin in a homogeneous aqueous-organic system. Bull. Chem. Soc. Jpn. 68: 1019-1023.
8. Kunugi, S., Koyasu, A., Kitayaki, M., Takahashi, S., Oda, K. 1996. Kinetic characterization of the neutral protease vimelysin from Vibrio sp. T1800. Eur. J. Biochem. in press.
9. Kunugi, S., Yoshida, M. 1996. Kinetics of thermolysin-catalyzed peptide formation reaction in acetonitrile-water. Bull. Chem. Soc. Jpn. 69: 805-809.
10. Lee, K. H., Lee, P. M., Siaw, Y. S., Morihara, K. 1992. Aspartame precursor synthesis in water miscible cosolvent catalyzed by thermolysin. Biotech. Lett. 14: 1159-1164.
11. Martinez, P., Van Dam, M. E., Robinson, A. C., Chen, K., Arnold, F. H. 1992. Stabilization of subtilisin E in organic solvents by site-directed mutagenesis. Biotechn. Bioeng. 39: 141-147.
12. Nakanishi, K., Matsuno, R. 1986. Kinetics of enzymatic synthesis of peptides in aqueous/organic biphasic systems. Eur. J. Biochem. 161: 533-540.
13. Oda, K., Okayama, K., Okutomi, K., Shimada, M., Sato, R., Takahashi, S. 1996. A novel alcohol resistant metalloproteinase, Vimelysin, from Vibrio sp. TI1800: Purification and characterization. Biosci. Biotech. Biochem. 60: 463-467.
14. Oka, T., Morihara, K. 1980. Peptide Bond Synthesis catalyzed by thermolysin. J. Biochem. (Tokyo) 88: 807-813.
15. Oyama, K., Kihara, K., Nonaka, Y. 1981. On the mechanism of the action of thermolysin. Kinetic study of the thermolysin-catalyzed condensation reaction of N-benzyloxycarbonyl-L-aspartic acid with L-phenylalanine methyl ester. J. Chem. Soc. Perkin 2: 356-360.
16. Takahashi, S., Okayama, K., Kunugi, S., Oda, K. 1996. Substrate specificity of a novel alcohol resistant metalloproteinase, vimelysin, from Vibrio sp. T1800. Biosci. Biotech. Biochem. 60:1651-1654.
17. Wangikar, P. P., Graycar, T. P., Estell, D. A., Clark, D. S., Dordick, J. S. 1993. Protein and solvent engineering of subtilisin BPN' in nearly anhydrous organic media. J. Am. Chem. Soc. 115: 12231-12237.
18. Wangikar, P. P., Rich, J. O., Clark, D. S., Dordick, J. S. 1995. Probing enzymic transition state hydrophobicity, Biochemistry 34: 121302-12310.
19. Wayne, S. I., Fruton, J. S. 1983. Thermolysin-catalyzed peptide bond synthesis. Proc. Natl. Acad. Sci. USA 80: 3241-3244.
20. Wong, C.-H., Chen, S. T., Hennen, M. W., Bibbs, J. A., Wang, Y.-F., Liu, J. L.-C., Pantoliano, M. W., Whitlow, M., Bryan, P. N. 1990. Enzymes in organic synthesis: Use of subtilisin and a highly stable mutant derived from multiple site-specific mutations. J. Am. Chem. Soc. 112: 945-953.
21. Zhong, Z., Liu, J. L.-C., Dinterman, L. M., Finkelman, M. A., Mueller, W. T., Rollence, M. L., Whitlow, M., Wong, C.-H. 1991. Engineering subtilisin for reaction in dimethylformamide. J. Am. Chem. Soc. 113: 683-684.