Electroelution as a simple and fast protein purification method: Isolation of an extracellular xylanase from Bacillus sp. CCMI 966

Enzyme and Microbial Technology

Volumn 27, Issue 1-2, 2000, Pages 95-99

  Sá Pereira, Paula ^a   Duarte, José ^a   Costa Ferreira, Maria ^a  

^a National Institute of Industrial Engineering and Technology   (Portugal)

Author keywords

Electroelution; High molecular weight xylanase; Purification

Indexed keywords

BACTERIA; ELECTROPHORESIS; ENZYMES; MOLECULAR WEIGHT; PURIFICATION;

BACILLUS SPECIES; ELECTROELUTION; POLYACRYLAMIDE GEL ELECTROPHORESIS; XYLANASE;

BIOTECHNOLOGY;

BACTERIAL ENZYME; POLYACRYLAMIDE GEL; XYLAN ENDO 1,3 BETA XYLOSIDASE;

ARTICLE; BACILLUS; CONTROLLED STUDY; ELECTROELUTION; ENZYME ACTIVITY; ENZYME PURIFICATION; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; TECHNIQUE;

BACILLUS;

EID: 0342264573     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(00)00185-X     Document Type: Article

References (28)

1. Coughlan M.P., Hazlewood G.P. β-1, 4-D-xylan degrading enzyme systems biochemistry, molecular biology and applications. Biotechnol Appl Biochem. 7:1993;259-289.
2. Duarte J.C., Costa-Ferreira M. Aspergilli and lignocellulosics enzymology and biotechnological applications. FEMS Microbiol Rev. 13:1994;377-386.
3. Kirk TK, Jeffries TW. Roles for microbial enzymes in pulp and paper processing. Enzymes for pulp and paper processing (American Chemical Society). 1996:2-14 (internet document).
4. Suurnäkki A., Tenkanen M., Buchert J., Viikari L. Hemicellulases in the bleaching of chemical pulps. Adv Biochem Eng Biotechnol. 57:1997;261-287.
5. Gilbert M, Breuil C, Yaguchi M, Saddler JN. Purification and characterization of a xylanase from thermophilic ascomycete Thelavia terrestris 255B. Appl Biochem Biotech 1992;34/35:247-59.
6. Nakamura S., Wakabayashi K., Nakai R., Aono R., Horikoshi K. Purification and some properties of an alkaline xylanase from alkaliphilic Bacillus sp. Strain 41 M-1. Appl Environ Microbiol. 59:1993;2311-2316.
7. Viikari L., Kantelinen A., Sundquist J., Linko M. Xylanases in bleaching from an idea to the industry. FEMS Microbiol Rev. 13:1994;335-350.
8. Blanco A., Vidal T., Colom J.F., Pastor F.I.J. Purification and properties of Xylanase A from alkali-tolerant Bacillus sp. strain BP-23. Appl Environ Microbiol. 61:1995;4468-4470.
9. Gilead S., Shoham Y. Purification of α-arabinofuranosidase from Bacillus stearothermophilus T-6. Appl Environ Microbiol. 61:1995;170-174.
10. Christapoulos P., Nerinckx W., Kekos D., Macris B., Claeyssens M. Purification of two low molecular mass alkaline xylanases from Fusarium oxysporum F3. J Biotechnol. 51:1996;181-189.
11. Breccia J.D., Siñeriz G.F., Baigorí M.D., Castro J.R., Hatti-Kaul R. Purification and characterization of a thermostable xylanase from Bacillus amyloliquefaciens. Enzyme MicrobTechnol. 22:1998;42-49.
12. Gessesse A. Purification of two thermostable alkaline xylanases from an alkaliphilic Bacillus sp. Appl Environ Microbiol. 64:1998;3533-3535.
13. Ratanakhanokchai K., Kyu K.L., Tanticharoen M. Purification and properties of a xylan-binding endoxylanase from alkaliphilic Bacillus sp. strain K-1. Appl Environ Microbiol. 65:1999;694-697.
14. Kulkarni N., Shendye A., Rao M. Molecular and biotechnological aspects of xylanases. FEMS Microbiol Rev. 23:1999;411-456.
15. Harrington M.G. Purification procedures electrophoretic methods elution of protein from gels. Deutscher M.P. Guide to protein purification. Methods in enzymology. 1990;488-498 Academic Press.
16. Bierbaum G., Karutz M., Weuster-Botz D., Wanbrey C. Production of protease with Bacillus licheniformis mutants insensitive to repression of exoenzyme biosynthesis. Appl Microbiol Biotechnol. 40:1994;611-617.
17. Yang V.W., Zhuang Z., Elegir G., Jeffries T.W. Alkaline-active xylanase produced by an alkaliphilic Bacillus sp isolated from kraft pulp. J Indus Microbiol. 15:1995;434-441.
18. Béguin P. Detection of cellulase activity in polyacrylamide gels using congo-red-stained agar replicas. Anal Biochem. 131:1983;333-336.
19. Bailey M., Biely P., Poutaten K. Interlaboratory testing of methods for assay of xylanase activity. J Biotechnol. 23:1992;257-270.
20. Miller G.L. Use of dinitrosalicylic acid agent for determination of reducing sugar. Anal Chem. 1959;31426-31428.
21. 4. Nature (London). 227:1970;680-685.
22. Merril C.R. Gel-staining techniques in guide to protein purification. Meth Enzymol. 182:1990;477-487.
23. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72:1976;248-254.
24. Kulkarni N., Vaidya A., Rao M. Extractive cultivation of recombinant Escherichia coli using aqueous two phase systems for production and separation of extracellular xylanase. Biochem Biophys Res Commun. 255:1999;274-278.
25. Sá-Pereira P. Characterization of a β-1, 4-endoglucanase codifying gene from Cellvibrio mixtus, expressed in Escherichia coli. (Abstract in English). Technical University of Lisbon, 1994. Thesis.
26. Archana A., Satyanarayana T. Xylanase production by thermophilic Bacillus licheniformis A99 in solid-state fermentation. Enzyme Microb Technol. 21:1997;12-17.
27. Nanmori T., Watanabe T., Shinke R., Kohno A., Kawamura Y. Purification and properties of thermostable xylanase and β-xylosidase produced by a newly isolate Bacillus stearothermophilus strain. J Bacteriol. 172:1990;6669-6672.
28. Rajaram S., Varma A. Production and characterization of xylanase from Bacillus thermoalkalophilus grown on agricultural wastes. Appl Microbiol Biotechnol. 34:1990;141-144.