Quantitative analysis of binding of transcription factor complex to biotinylated DNA probe by a streptavidin-agarose pulldown assay

Analytical Biochemistry

Volumn 323, Issue 1, 2003, Pages 12-18

  Deng, Wu Guo ^a   Zhu, Ying ^a,b   Montero, Alberto ^a   Wu, Kenneth K ^a  

^a UNIVERSITY OF TEXAS MEDICAL SCHOOL   (United States)

^b WUHAN UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CELL CULTURE; CELL DEATH; PELLETIZING; PROBES; PROTEINS; TRANSCRIPTION FACTORS;

BIOTINYLATED DNA; CREB BINDING PROTEINS; CYCLOOXYGENASE 2; HUMAN FIBROBLAST; INDIVIDUAL PROTEINS; NUCLEAR EXTRACTS; STREPTAVIDIN BEADS; TUMOR NECROSIS FACTOR ALPHA;

TRANSCRIPTION;

AGAROSE; CHICKEN OVALBUMIN UPSTREAM PROMOTER TRANSCRIPTION FACTOR; CYCLIC AMP RESPONSIVE ELEMENT BINDING PROTEIN BINDING PROTEIN; CYCLOOXYGENASE 2; HISTONE ACETYLTRANSFERASE PCAF; PHORBOL ESTER; PROTEIN; PROTEIN MED7; PROTEIN P300; PROTEIN SRB7; STREPTAVIDIN; TRANSCRIPTION FACTOR; TUMOR NECROSIS FACTOR ALPHA; UNCLASSIFIED DRUG;

ARTICLE; BINDING ASSAY; BIOTINYLATION; CELL NUCLEUS; CENTRIFUGATION; CONTROLLED STUDY; DNA PROBE; DNA PROTEIN COMPLEX; ENZYME ACTIVATION; EVALUATION; FIBROBLAST; GENE ACTIVATION; GENE EXPRESSION REGULATION; HUMAN; HUMAN CELL; IMMUNOBLOTTING; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN FUNCTION; QUANTITATIVE ANALYSIS; ROOM TEMPERATURE;

EID: 0242690439     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2003.08.007     Document Type: Article

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