A modular cross-linking approach for exploring protein interactions

Journal of the American Chemical Society

Volumn 125, Issue 9, 2003, Pages 2416-2425

  Trester Zedlitz, Michelle ^a   Kamada, Katsuhiko ^a   Burley, Stephen K ^b   Fenyö, David ^c   Chait, Brian T ^a   Muir, Tom W ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

^b ELI LILLY AND COMPANY   (United States)

^c Amersham Biosciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPUTATIONAL METHODS; CROSSLINKING; MASS SPECTROMETRY;

PROTEIN INTERACTIONS;

PROTEINS;

PROTEIN; REAGENT;

ARTICLE; MASS SPECTROMETRY; MATHEMATICAL COMPUTING; PROTEIN CROSS LINKING; PROTEIN PROTEIN INTERACTION; SYNTHESIS; TECHNIQUE;

ACRYLIC RESINS; AMINO ACID SEQUENCE; BIOTIN; CROSS-LINKING REAGENTS; GLYCINE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PHOSPHOPROTEINS; POLYETHYLENE GLYCOLS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SULFHYDRYL COMPOUNDS; TRANSCRIPTION FACTORS;

EID: 0242669340     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja026917a     Document Type: Article

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26. Observation of the cross-linked product requires that only one of the three be intermolecular, The other two links can be either inter- or intramolecular.
27. Note: Some sample loss on filters should be anticipated.
28. Abbreviations: Boc, tert-butyloxycarbonyl; di-Boc, di-tert-butyl dicarbonate; DIEA, N,N-diisopropylethylamine; EDC, N-ethyl-N′-(3-dimethylaminopropyl)carbodiimide; HBTU, 2-[1H-benzotriazolyl]-1,1,3,3-tetramethyluronium hexafluorophosphate; HPLC, high-performance liquid chromatography; MALDI, matrix-assisted laser desorption ionization; NHS, N-hydroxysuccinimide; PEGA, poly(ethylene glycol)-poly(N,N-dimethylacrylamide); PyBOP, (benzotriazol-1-yloxy)tripyrrol/dinophosphonium hexafluorophosphate; SPS, solid-phase synthesis: sulfo-NHS, sulfo-N-hydroxysuccinimide; TDA, 4,7,10-trioxatridecane-11,13-diamine. Standard IUPAC single and triple letter codes for amino acids are used throughout.
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31. The truncated NC2 α protein contains residues 11-95 of the native protein in addition to an N-terminal leader sequence (GPLGS).
32. The truncated NC2 β protein contains residues 7-99 of the native protein in addition to a Met at its N-terminus.
33. Complete cross-links include those that occur between different proteolytic peptide fragments as well as those that occur within a given peptide. Incomplete or dead-end cross-links occur only within single peptide fragments.