Is dimethylsulfoxide a reliable solvent for extracting chlorophyll under field conditions?

Photosynthesis Research

Volumn 78, Issue 1, 2003, Pages 87-91

  Tait, Michelle A ^a   Hik, David S ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

Acetone; Chlorophyll extraction; Dimethylsulfoxide; DMSO; Field methodology

Indexed keywords

EID: 0242662067     PISSN: 01668595     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1026045624155     Document Type: Article

References (22)

1. Alpert P (1984) Analysis of chlorophyll content in mosses though extraction in DMSO. Bryologist 87: 363-365
2. Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidases in Beta vulgaris. Plant Physiol 24: 1-15
3. Barnes JD, Balaguer L, Manrique E, Elvira S and Davison AW (1992) A reappraisal of the use of DMSO for the extraction and determination of chlorophyll a and b in lichens and higher plants. Environ Exp Bot 32: 85-100
4. Garcia AL and Nicolas N (1998a) Establishing errors in the spectrophotometric determination of chlorophylls using different solvents. J Plant Physiol 152: 392-398
5. Garcia AL and Nicolas N (1998b) Influence of the degree of solvent impurity on the spectrophotometric determination of chlorophylls in 80% aqueous acetone and dimethyl formamide. Application to non-abrasive extraction of leaves of Citrus aurantium. Photosynthetica 35: 545-550
6. Hik DS, McColl C and Boonstra R (2001) Why are Arctic ground squirrels more stressed in the boreal forest than in alpine meadows? EcoScience 8: 275-288
7. Hiscox JD and Israelstam GF (1979) A method for the extraction of chlorophyll from leaf tissue without maceration. Can J Bot 57: 1332-1334
8. Inskeep WP and Bloom PR (1985) Extinction coefficients of chlorophyll a and b in N,N-dimethylformamide and 80% acetone. Plant Physiol 77: 483-485
9. Moran R and Porath D (1980) Chlorophyll determination in intact tissues using N,N-dimethylformamide. Plant Physiol 65: 478-479
10. Porra RJ (2002) The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b. Photosynth Res 73: 149-156
11. Porra RJ, Thompson WA and Kriedmann PE (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: Verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim Biophys Acta 975: 384-394
12. Ronen R and Galun M (1984) Pigment extraction from lichens with dimethyl sulfoxide (DMSO) and estimation of chlorophyll degradation. Environ Exp Bot 24: 239-245
13. Scott BJ and Robson AD (1991) Assessment of dimethyl-sulfoxide as a chlorophyll extractant and for the partitioning of magnesium. Commun Soil Sci Plant Anal 22: 701-711
14. Shinano T, Lei TT, Kawamukai T, Inoue MT, Koike T and Tadano T (1996) Dimethylsulfoxide method for the extraction of chlorophylls a and b from the leaves of wheat, field bean, dwarf bamboo, and oak. Photosynthetica 32: 409-415
15. Shoaf TW and Lium BW (1976) Improved extraction of chlorophyll a and b from algae using dimethyl sulphoxide. Limnol Oceanogr 21: 926-928
16. Spencer DF and Ksander GG (1987) Comparison of thee methods for extracting chlorophyll from aquatic macrophytes. J Freshwater Ecol 4: 201-208
17. Speziale BJ, Scheiner SP, Giammatteo PA and Schindler JE (1984) Comparison of N,N-dimethylformadide, dimethylsulfoxide, and acetone for extraction of phytoplankton chlorophyll. Can J Fish Aquat Sci 41: 1519-1522
18. Stauffer RE, Lee GF and Armstrong DE (1979) Estimating chlorophyll extraction biases. J Fish Res Board Can 36: 152-157
19. Tait MA (2002) The effects of climate change and herbivory on alpine plants in the southwest Yukon. MSc Thesis, University of Alberta, Edmonton, Alberta, Canada
20. Thayer SS and Björkman O (1990) Leaf xanthopyll content and composition in sun and shade determined by HPLC. Photosynth Res 23: 331-343
21. Wellburn AR (1994) The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol 144: 307-313
22. Yoder BJ and Daley LS (1989) Development of a visible spectroscopic method for determining chlorophyll a and b in vivo in leaf samples. Spectroscopy 8: 44-50