Sequential cloned gene integration in the yeast Kluyveromyces lactis

Applied Microbiology and Biotechnology

Volumn 62, Issue 5-6, 2003, Pages 523-527

  Wang, Y C M ^a   Chuang, L L ^a,b   Lee, F W F ^a,c   Da Silva, N A ^a  

^a University of California at Irvine   (United States)

^b WAYNE STATE UNIVERSITY   (United States)

^c CENTOCOR INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTECHNOLOGY; YEAST;

ARTICLE; CLONING VECTOR; CUP1 GENE; DELTA INTEGRATING VECTOR; DELTA UB INTEGRATING VECTOR; DNA INTEGRATION; GENE; GENE INSERTION; GENE NUMBER; KLUYVEROMYCES; LACZ GENE; MOLECULAR CLONING; NONHUMAN; PDELTA LACZ NEO VECTOR; PDELTA LACZ UB VECTOR; PROMOTER REGION;

BETA-GALACTOSIDASE; CHROMOSOMES, FUNGAL; CLONING, MOLECULAR; DNA, RECOMBINANT; GENE DOSAGE; GENE EXPRESSION; GENETIC VECTORS; KLUYVEROMYCES; RECOMBINATION, GENETIC; RETROELEMENTS; TRANSFORMATION, GENETIC;

FUNGI; INSERTION SEQUENCES; KLUYVEROMYCES; KLUYVEROMYCES LACTIS; MYXOGASTRIA; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; UNIDENTIFIED CLONING VECTOR;

EID: 0242659248     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-003-1319-2     Document Type: Article

References (33)

1. Alani E, Cao L, Kleckner N (1987) A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics 116; 541-545
2. Bergkamp RJM, Kool IM, Geerse RH, Planta RJ (1992) Multiple-copy integration of the α-galactosidase gene from Cyamopsis tetragonoloba into the ribosomal DNA of Kluyveromyces lactis. Curr Genet 21:365-370
3. Bianchi M, Falcone C, Chen X, Weslowski-Louvel M, Frontali L, Fukuhara H (1987) Transformation of the yeast Kluyveromyces lactis by new vectors derived from the 1.6 μm circular plasmid pKD1. Curr Genet 12:185-192
4. Bianchi MM, Santarelli R, Frontali L (1989) Active recombination of pKD1-derived vectors with resident pKD1 in Kluyveromyces lactis transformation. Curr Genet 15:253-260
5. Boeke JD, Garfinkel DJ, Styles CA, Fink GR (1985) Ty elements transpose through an RNA intermediate. Cell 40:491-500
6. Bolotin-Fukuhara M, Toffano-Nioche C, Artiguenave F, Duchateau-Nguyen G, Lemaire M, Marmeisse R, Montrocher R, Robert C, Termier M, Wincker P, Wésolowski-Louvel M (2000) Genomic exploration of the hemiascomycetous yeasts: 11. Kluyveromyces lactis. FEBS Lett 487:66-70
7. Bonekamp FJ, Oosterom J (1994) On the safety of Kluyveromyces lactis - a review. Appl Microbiol Biotechnol 41:1-3
8. Bundock P, Mroczek K, Winkler AA, Steensma HY, Hooykaas PJJ (1999) T-DNA from Agrobacterium tumefaciens as an efficient tool for gene targeting in Kluyveromyces lactis. Mol Gen Genet 261:115-121
9. Chen XJ (1996) Low-and high-copy-number shuttle vectors for replication in the budding yeast Kluyveromyces lactis. Gene 172:131-136
10. Chen XJ, Fukuhara H (1988) A gene fusion system using the aminoglycoside 3′-phosphotransferase gene of the kanamycin-resistance transposon Tn903: use in the yeasts Kluyveromyces lactis and Saccharomyces cerevisiae. Gene 69:181-192
11. Da Silva NA, Bailey JE (1989) Construction and characterization of a temperature-sensitive expression system in recombinant yeast. Biotechnol Prog 5:18-26
12. Dujon B (1996) The yeast genome project: What did we learn? Trends Genet 12:263-270
13. Durrin LK, Mann RK, Kayne PS, Grunstein M (1991) Yeast histone H4 N-terminal sequence is required for promoter activation in vivo. Cell 65:1023-1031
14. Fleer R, Chen X, Amellal N, Yeh P, Fournier A, Guinet F, Gault N, Faucher D, Folliard F, Fukuhara H, Mayaux J (1991a) High-level secretion of correctly processed recombinant human interleukin-1β in Kluyveromyces lactis. Gene 107:285-295
15. Fleer R, Yeh P, Amellal N, Maury I, Fournier A, Bacchetta F, Baduel P, Jung G, L'Hote H, Becquart J, Fukuhara H, Mayayaux J-F (1991b) Stable multicopy vectors for high-level secretion of recombinant human serum albumin by Kluyveromyces lactis yeasts. Bio/Technol 9:968-975
16. Hsieh H-P, Da Silva NA (1998) Partial-pKD1 plasmids provide enhanced structure stability for heterologous protein production in Kluyveromyces lactis. Appl Microbiol Biotechnol 49:411-416
17. Ito H, Fukuda Y, Murata K, Kimura A (1983) Transformation of intact yeast cells treated with alkali cations. J Bacteriol 153:163-168
18. Lee FWF, Da Silva NA (1997a) Improved efficiency and stability of multiple cloned gene insertions at the 5 sequences of Saccharomyces cerevisiae. Appl Microbiol Biotechnol 48:339-345
19. Lee FWF, Da Silva NA (1997b) Sequential δ-integration for the regulated insertion of cloned genes in Saccharomyces cerevisiae. Biotechnol Prog 13:368-373
20. Macreadie IG, Horaitis O, Vaughan PR, Clark-Walker GD (1991) Constitutive expression of the Saccharomyces cerevisiae CUP1 gene in Kluyveromyces lactis. Yeast 7:127-135
21. Mulder W, Scholten IHJM, Grivell LA (1995) Carbon catabolite regulation of transcription of nuclear genes coding for mitochondrial proteins in the yeast Kluyveromyces lactis. Curr Genet 28:267-273
22. Porro D, Bianchi MM, Brambilla L, Menghini R, Bolzani D, Carrera V, Lievense J, Liu C-L, Ranzo BM, Frontali L, Alberghina L (1999) Replacement of a metabolic pathway for large-scale production of lactic acid from engineered yeasts. Appl Env Microbiol 65:4211-4215
23. Randolph W (1984) Direct food substance affirmed as generally recognized as safe: lactase enzyme preparation from Kluyveromyces lactis. Fed Reg 49:47384-47387
24. Rech EL, Dobson MJ, Davey MR, Mulligan BJ (1990) Introduction of a yeast artificial chromosome vector into Saccharomyces cerevisiae cells by electroporation. Nucleic Acids Res 18:1313
25. Romanos MA, Scorer CA, Clare JJ (1992) Foreign gene expression in yeast: a review. Yeast 8:423-488
26. Rossolini GM, Riccio ML, Gallo E, Galeotti CL (1992) Kluyveromyces lactis rDNA as a target for multiple integration by homologous recombination. Gene 119:75-81
27. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
28. Sherman F, Fink GR, Hicks J (1986) Methods in yeast genetics. Cold Spring Harbor, Cold Spring Harbor Laboratory, New York
29. Srienc F, Campbell JL, Bailey JE (1983) Detection of bacterial β-galactosidase activity in individual Saccharomyces cerevisiae cells by flow cytometry. Biotechnol Lett 5:43-48
30. Swinkels BW, van Ooyen AJJ, Bonekamp FJ (1993) The yeast Kluyveromyces lactis as an efficient host for heterologous gene expression. Antonie van Leeuwenhoek 64:187-201
31. Van der Berg J, van der Laken K, van Ooyen A, Renniers T, Rietvld K, Schaap A, Brake A, Bishop R, Schutz K, Moyer D, Richma, M, Shuste, J (1990) Kluyveromyces as a host for heterologous gene expression: expression and secretion of prochymosin. Bio/Technol 8:185-192
32. Wang X, Wang Z, Da Silva NA (1996) G418 selection and stability of cloned genes integrated at the chromosomal δ sequences of Saccharomyces cerevisiae. Biotechnol Bioeng 49:45-51
33. Wésolowski-Louvel M, Breunig KD, Fukuhara H (1996) Kluyveromyces lactis. In: Wolf K (ed) Genetics, biochemistry, and molecular biology of non-conventional yeasts. Springer, Berlin Heidelberg New York, pp 139-201