A Simple Quenching Method for Fluorescence Background Reduction and Its Application to the Direct, Quantitative Detection of Specific mRNA

Analytical Chemistry

Volumn 75, Issue 22, 2003, Pages 6236-6243

  Nolan, Rhiannon L ^a   Cai, Hong ^a   Nolan, John P ^a   Goodwin, Peter M ^a  

^a LOS ALAMOS NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

TARGET DETECTION;

AMPLIFICATION; BIOASSAY; ENZYME KINETICS; FLUORESCENCE; GENES; OLIGOMERS; PROBLEM SOLVING; QUENCHING; REDUCTION;

RNA;

BETA ACTIN; MESSENGER RNA; NUCLEIC ACID; OLIGOMER; RNA;

ARTICLE; COLOR; CONCENTRATION (PARAMETERS); ENERGY TRANSFER; EXPERIMENT; FLUORESCENCE; GENOME; NUCLEIC ACID PROBE; QUALITY CONTROL; QUANTITATIVE ASSAY; RNA ANALYSIS; RNA HYBRIDIZATION; RNA SEQUENCE; SENSITIVITY AND SPECIFICITY; SIGNAL NOISE RATIO; SIGNAL PROCESSING;

ACTINS; BASE SEQUENCE; CELL LINE; FLUORESCENCE; FLUOROMETRY; HUMANS; MOLECULAR PROBE TECHNIQUES; OLIGONUCLEOTIDE PROBES; RNA, MESSENGER; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, FLUORESCENCE;

EID: 0242653730     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac034803r     Document Type: Article

References (27)

1. Epstein, J. R.; Biran, I.; Walt, D. R. Anal. Chim. Acta 2002, 469, 3-36.
2. Morrison, L. J. Fluorescence 1999, 7, 187-196.
3. Tyagi, S.; Kramer, F. R. Nat. Biotechnol. 1996, 14, 303-308.
4. Heid, C. A.; Stevens, J.; Livak, K. J.; Williams, P. M. Genome Res. 1996, 6, 986-994.
5. Andras, S. C.; Power, J. B.; Cocking, E. C.; Davey, M. R. Mol. Biotechnol. 2001, 19, 29-44.
6. Bustin, S. A. J. Mol. Endocrinol. 2002, 29, 23-39.
7. de Arruda, M.; Lyamichev, V. I.; Eis, P. S.; Iszczyszyn, W.; Kwiatkowski, R. W.; Law, S. M.; Olson, M. C.; Rasmussen, E. B. Expert Rev. Mol. Diagn. 2002, 2, 487-496.
8. Hirschfeld, T. Appl. Opt. 1976, 15, 2965-2966.
9. Keller, R. A.; Ambrose, W. P.; Goodwin, P. M.; Jett, J. H.; Martin, J. C.; Wu, M. Appl. Spectrosc. 1996, 50, A12-A32.
10. Xie, X. S.; Trautman, J. K. Annu. Rev. Phys. Chem. 1998, 49, 441-480.
11. Ambrose, W. P.; Goodwin, P. M.; Jett, J. H.; Van Orden, A.; Werner, J. H.; Keller, R. A. Chem. Rev. 1999, 99, 2929-2956.
12. Keller, R. A.; Ambrose, W. P.; Arias, A. A.; Gai, H.; Emory, S. R.; Goodwin, P. M.; Jett, J. H. Anal. Chem. 2002, 74, 316a-324a.
13. Castro, A.; Williams, J. G. K. Anal. Chem. 1997, 69, 3915-3920.
14. Knemeyer, J.-P.; Marmé, N.; Sauer, M. Anal. Chem. 2000, 72, 3717-3724.
15. Anazawa, T.; Matsunaga, H.; Yeung, E. S. Anal. Chem. 2002, 74, 5033-5038.
16. Goodwin, P. M.; Nolan, R. L.; Cai, H. Proc. SPIE 2003, 4962, 78-88.
17. Li, H. T.; Ying, L. M.; Green, J. J.; Balasubramanian, S.; Klenerman, D. Anal. Chem. 2003, 75, 1664-1670.
18. Korn, K.; Gardellin, P.; Liao, B.; Amacker, M.; Bergström, A.; Björkman, H.; Camacho, A.; Dörhöfer, S.; Dörre, K.; Enström, J.; Ericson, T.; Favez, T.; Gösch, M.; Honegger, A.; Jaccoud, S.; Lapczyna, M.; Litborn, E.; Thyberg, P.; Winter, H.; Rigler, R. Nucleic Acids Res. 2003, 31, e89.
19. Breslauer, K. J.; Frank, R.; Blocker, H.; Marky, L. A. Proc. Natl. Acad. Sci. U. S.A. 1986, 83, 3746-3750.
20. Sugimoto, N.; Nakano, S.; Yoneyama, M.; Honda, K. Nucleic Acids Res. 1996, 24, 4501-4505.
21. Schwille, P.; Meyer-Almes, F.-J.; Rigler, R. Biophys. J. 1997, 72, 1878-1886.
22. Schwille, P. In Fluorescence Correlation Spectroscopy, Theory and Applications; Rigler, R., Elson, E., Eds.; Springer: Berlin, 2000, pp 360-378.
23. Levene, M. J.; Korlach, J.; Turner, S. W.; Foquet, M.; Craighead, H. G.; Webb, W. W. Science 2003, 299, 682-686.
24. Shera, E. B.; Seitzinger, N. K.; Davis, L. M.; Keller, R. A.; Soper, S. A. Chem. Phys. Lett. 1990, 174, 553-557.
25. Tsuchiya, S.; Yamabe, M.; Yamaguchi, Y.; Kobayashi, Y.; Konno, T.; Tada, K. Int. J. Can. 1980, 26, 171-176.
26. Strong, R. C.; Korsmeyer, S. J.; Parkin, J. L.; Arthur, D. C.; Kersey, J. H. Blood 1985, 65, 21-31.
27. Lupberger, J.; Kreuzer, K. A.; Baskaynak, G.; Peters, U. R.; le Coutre, P.; Schmidt, C. A. Mol. Cell. Probes 2002, 16, 25-30.