Dual-color real-time telomeric repeat amplification protocol

BioTechniques

Volumn 35, Issue 5, 2003, Pages 912-914

  Fajkus, Jiří ^a,b   Koppová, Květa ^b   Kunická, Zuzana ^b  

^a INSTITUTE OF BIOPHYSICS   (Czech Republic)

^b MASARYK UNIVERSITY   (Czech Republic)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; EXONUCLEASE; FLUORESCEIN; METHYL ORANGE; SULFORHODAMINE B; TELOMERASE; TUMOR MARKER;

ARTICLE; COLOR; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUOROMETRY; HELA CELL; HUMAN; IMMUNOMAGNETIC SEPARATION; MYELOMA; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; REGRESSION ANALYSIS; TELOMERIC REPEAT AMPLIFICATION PROTOCOL;

COLOR; FLUORESCEIN; GENE AMPLIFICATION; NUCLEIC ACID AMPLIFICATION TECHNIQUES; ONLINE SYSTEMS; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; REPRODUCIBILITY OF RESULTS; RHODAMINES; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, FLUORESCENCE; TELOMERASE; TELOMERE; TERMINAL REPEAT SEQUENCES;

EID: 0242526122     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (4)

1. Fajkus, J., M. Simickova, and J. Malaska. 2002. Tiptoeing to chromosome tips: facts, promises and perils of today's human telomere biology. Philos. Trans. R. Soc. Lond. B Biol. Sci. 357:545-562.
2. Kim, N.W., M.A. Piatyszek, K.R. Prowse, C.B. Harley, M.D. West, P.L. Ho, G.M. Coviello, W.E. Wright, et al. 1994. Specific association of human telomerase activity with immortal cells and cancer. Science 266:2011-2015.
3. Wege, H., M.S. Chui, H.T. Le, J.M. Tran, and M.A. Zern. 2003. SYBR Green real-time telomeric repeat amplification protocol for the rapid quantification of telomerase activity. Nucleic Acids Res. 31:E3-3.
4. Wu, K.D., L.M. Orme, J. Shaughnessy, Jr., J. Jacobson, B. Barlogie, and M.A.S. Moore. 2003. Telomerase and telomere length in multiple myeloma: correlations with disease heterogeneity, cytogenetic status, and overall survival. Blood 101:4982-4989.