Comparison of DNA Amplification, mRNA Amplification, and DNA Hybridization Techniques for Detection of Cytomegalovirus in Bone Marrow Transplant Recipients

Journal of Clinical Microbiology

Volumn 41, Issue 11, 2003, Pages 5159-5166

  Diaz Mitoma, Francisco ^a   Leger, Chantal ^b   Miller, Helen ^a   Giulivi, Antonio ^b,c   Frost, Rita ^a   Shaw, Laura ^a   Huebsch, Lothar ^b  

^a CHILDREN S HOSPITAL OF EASTERN ONTARIO   (Canada)

^b UNIVERSITY OF OTTAWA   (Canada)

^c HEALTH CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS DNA; VIRUS MESSENGER RNA;

ADOLESCENT; ADULT; ANALYTIC METHOD; ARTICLE; ASSAY; BONE MARROW TRANSPLANTATION; CYTOMEGALOVIRUS; DNA HYBRIDIZATION; FEMALE; GENE AMPLIFICATION; HUMAN; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MALE; MULTIVARIATE ANALYSIS; PRIORITY JOURNAL; RISK FACTOR; SENSITIVITY AND SPECIFICITY; VIRUS DETECTION; VIRUS LOAD; VIRUS REPLICATION;

ADOLESCENT; ADULT; BONE MARROW TRANSPLANTATION; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; DEMOGRAPHY; DNA, VIRAL; FEMALE; HUMANS; MALE; MIDDLE AGED; NUCLEIC ACID HYBRIDIZATION; POSTOPERATIVE COMPLICATIONS; RETROSPECTIVE STUDIES; RNA, MESSENGER; RNA, VIRAL; ROC CURVE; SENSITIVITY AND SPECIFICITY; TRANSPLANTATION, HOMOLOGOUS; TREATMENT OUTCOME;

CUCUMBER MOSAIC VIRUS; CYTOMEGALOVIRUS; DNA VIRUSES; RNA VIRUSES;

EID: 0242509122     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.41.11.5159-5166.2003     Document Type: Article

References (29)

1. Avery, R. K., K. A. Adal, D. L. Longworth, and B. J. Bolwell. 2000. A survey of allogeneic bone marrow transplant programs in the United States regarding cytomegalovirus prophylaxis and pre-emptive therapy. Bone Marrow Transplant. 26:763-767.
2. Barrett-Muir, W., J. Breuer, C. Millar, J. Thomas, D. Jeffries, M. Yaqoob, and C. Aitken. 2000. CMV viral load measurements in whole blood and plasma-which is best following renal transplantation? Transplantation 70:116-119.
3. Bhorade, S. M., C. Sandesara, E. R. Garrity, W. T. Vigneswaran, L. Norwick, S. Alkan, A. N. Husain, M. A. McCabe, and V. Yeldandi. 2001. Quantification of cytomegalovirus (CMV) viral load by the hybrid capture assay allows for early detection of CMV disease in lung transplant recipients. J. Heart Lung Transplant. 20:928-934.
4. Blank, B. S. N., P. L. Meenhorst, J. W. Mulder, G. J. Weverling, H. Putter, W. Pauw, W. C. van Dijk, P. Smits, S. Lie-A-Ling, P. Reiss, and J. M. Lange. 2000. Value of different assays for detection of human cytomegalovirus (HCMV) in predicting the development of HCMV disease in human immunodeficiency virus-infected patients. J. Clin. Microbiol. 38:563-569.
5. Blok, M. J., I. Lautenschlager, V. J. Goossens, J. M. Middeldorp, C. Vink, K. Hockerstedt, and C. A. Bruggeman. 2000. Diagnostic implications of human cytomegalovirus immediate early-1 and pp67 mRNA detection in whole-blood samples from liver transplant patients using nucleic acid sequence-based amplification. J. Clin. Microbiol. 38:4485-4491.
6. Boivin, G., R. Bélanger, R. Delage, C. Beliveau, C. Demers, N. Goyette, and J. Roy. 2000. Quantitative analysis of cytomegalovirus (CMV) viremia using the pp65 antigenemia assay and the COBAS AMPLICOR CMV MONITOR PCR test after blood and marrow allogeneic transplantation. J. Clin. Microbiol. 38:4356-4360.
7. Boivin, G., J. Handfield, E. Toma, G. Murray, R. Lalonde, V. J. Tevere, R. Sun, and M. G. Bergeron. 1998. Evaluation of the AMPLICOR cytomegalovirus test with specimens from human immunodeficiency virus-infected subjects. J. Clin. Microbiol. 36:2509-2513.
8. Emery, V. C., C. A. Sabin, A. V. Cope, D. Gor, A. F. Bassan Hassan Walker, and P. D. Griffiths. 2000. Application of viral-load kinetics to identify patients who develop cytomegalovirus disease after transplantation. Lancet 355:2032-2036.
9. Gerna, G., F. Baldanti, J. Middeldorp, and D. Lilleri. 2000, Use of CMV transcripts for monitoring CMV infections in transplant patients. Int. J. Antimicrob. Agents 16:455-460.
10. Goossens, V. J., C. Vink, W. Mullers, J. M. Middeldorp, and C. A. Bruggeman. 2001. Different profiles of cytomegalovirus RNA transcripts and anti-cytomegalovirus IgM antibodies in renal transplant recipients. J. Clin. Virol. 23:87-95.
11. Gor, D., C. Sabin, H. G. Prentice, N. Vyas, S. Man, P. D. Griffiths, and V. C. Emery. 1998. Longitudinal fluctuations in cytomegalovirus load in bone marrow transplant patients: relationship between peak virus load, donor/recipient serostatus, acute GVHD and CMV disease. Bone Marrow Transplant. 21:597-605.
12. Halwachs-Baumann, G., M. Wilders-Truschnig, G. Enzinger, M. Eibl, W. Linkesch, H. J. Dornbusch, B. I. Santner, E. Marth, and H. H. Kessler. 2001. Cytomegalovirus diagnosis in renal and bone marrow transplant recipients: the impact of molecular assays. J. Clin. Virol. 20:49-57.
13. Hebart, H., A. Schröder, J. Löffler, T. Klingebiel, H. Martin, B. Wassmann, F. Gerneth, H. Rabenau, G. Jahn, L. Kanz, C. A. Muller, and H. Einsele. 1997. Cytomegalovirus monitoring by polymerase chain reaction of whole blood samples from patients undergoing autologous bone marrow or peripheral blood progenitor cell transplantation. J. Infect. Dis. 175:1490-1493.
14. Jabs, D. A., M. Forman, C. Enger, and J. B. Jackson. 1999. Comparison of cytomegalovirus loads in plasma and leukocytes of patients with cytomegalovirus retinitis. J. Clin. Microbiol. 37:1431-1435.
15. Ljungman, P., and S. A. Plotkin. 1995. Workshop on CMV disease; definitions, clinical severity scores, and new syndromes. Scand. J. Infect. Dis. Suppl. 99:87-89.
16. Mazzulli, T., L. W. Drew, B. Yen-Lieberman, D. Jekic-McMullen, D. J. Kohn, C. Isada, G. Moussa, R. Chua, and S. Walmsley. 1999. Multicenter comparison of the Digene hybrid capture CMV DNA assay (version 2.0), the pp65 antigenemia assay, and cell culture for detection of cytomegalovirus viremia. J. Clin. Microbiol. 37:958-963.
17. Metz, C. E. 1998. Basic principles of ROC analysis. Semin. Nucl. Med. 8:283-298.
18. Nichols, W. G., L. Corey, T. Gooley, W. L. Drew, R. Miner, M. Huang, C. Davis, and M. Boeckh. 2001. Rising pp65 antigenemia during preemptive anticytomegalovirus therapy after allogeneic hematopoietic stem cell transplantation: risk factors, correlation with DNA load, and outcomes. Blood 97:867-874.
19. Pellegrin, I., I. Garrigue, C. Binquet, G. Chene, D. Neau, P. Bonot, F. Bonnet, H. Fleury, and J. L. Pellegrin. 1999. Evaluation of new quantitative assays for diagnosis and monitoring of cytomegalovirus disease in human immunodeficiency virus-positive patients. J. Clin. Microbiol. 37:3124-3132.
20. Pellegrin, I., I. Garrigue, D. Ekouevi, L. Couzi, P. Merville, P. Merel, G. Chene, M. H. Schrive, P. Trimoulet, M. E. Lafon, and H. Fleury. 2000. New molecular assays to predict occurrence of cytomegalovirus disease in renal transplant recipients. J. Infect. Dis. 182:36-42.
21. Preiser, W., S. Bräuninger, R. Schwerdtfeger, U. Ayliffe, J. A. Garson, N. S. Brink, S. Franck, H. W. Doerr, and H. F. Rabenau. 2001. Evaluation of diagnostic methods for the detection of cytomegalovirus in recipients of allogeneic stem cell transplants. J. Clin. Virol. 20:59-70.
22. Schafer, P., W. Tenschert, L. Cremaschi, M. Schroter, B. Zollner, and R. Laufs. 2001. Area under the viraemia curve versus absolute viral load: utility for predicting symptomatic cytomegalovirus infections in kidney transplant patients. J. Med. Virol. 65:85-89.
23. Sia, I. G., J. A. Wilson, M. J. Espy, C. V. Paya, and T. F. Smith. 2000. Evaluation of the COBAS AMPLICOR CMV MONITOR test for detection of viral DNA in specimens taken from patients after liver transplantation. J. Clin. Microbiol. 38:600-606.
24. Tong, C. Y. W., L. E. Cuevas, H. Williams, and A. Bakran. 2000. Comparison of two commercial methods for measurement of cytomegalovirus load in blood samples after renal transplantation. J. Clin. Microbiol. 38:1209-1213.
25. Velzing, J., P. H. Rothbarth, A. C. M. Kroes, and W. G. V. Quint. 1994. Detection of cytomegalovirus mRNA and DNA encoding the immediate early gene in peripheral blood leukocytes from immunocompromised patients. J. Med. Virol. 42:164-169.
26. Wattanamano, P., J. L. Clayton, J. J. Kopicko, P. Kissinger, S. Elliot, C. Jarrott, S. Rangan, and M. A. Beilke. 2000. Comparison of three assays for cytomegalovirus detection in AIDS patients at risk for retinitis. J. Clin. Microbiol. 38:727-732.
27. Wolff, D., M. Skourtopoulos, D. Hörnschemeyer, C. Wolff, M. Korner, and R. Korfer. 1996. Longitudinal monitoring of latent and active human cytomegalovirus infections in peripheral blood of heart transplant recipients by single-tube nested RT-PCR. Microbiol. Res. 151:343-349.
28. Yen-Leiberman, B. 2000. Diagnosis of human cytomegalovirus disease. Clin. Microbiol. Newsl. 22(14):1-5.
29. Zipeto, D., S. Morris, C. Hong, A. Dowling, R. Wolitz, T. C. Merigan, and L. Rasmussen. 1995. Human cytomegalovirus (CMV) DNA in plasma reflects quantity of CMV DNA present in leukocytes. J. Clin. Microbiol. 33:2607-2611.