Nested multiplex RT-PCR for detection and differentiation of West Nile virus and eastern equine encephalomyelitis virus in brain tissues

Journal of Veterinary Diagnostic Investigation

Volumn 15, Issue 5, 2003, Pages 488-493

  Johnson, Donna J ^a   Ostlund, Eileen N ^a   Schmitt, Beverly J ^a  

^a National Veterinary Services Laboratories   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMALIA; AVES; EASTERN EQUINE ENCEPHALITIS VIRUS; EQUIDAE; EQUUS CABALLUS; FLAVIVIRUS; MAMMALIA; WEST NILE VIRUS;

EID: 0242492812     PISSN: 10406387     EISSN: None     Source Type: Journal    
DOI: 10.1177/104063870301500518     Document Type: Article

References (7)

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2. Henegariu O, Heerema NA, Dlouhy SR, et al.: 1997, Multiplex PCR: critical parameters and step-by-step protocol. Biotechniques 23:504-511.
3. Johnson DJ, Ostlund EN, Pedersen DD, Schmitt BJ: 2001, Detection of North American West Nile virus in animal tissue by a reverse transcription-nested polymerase chain reaction assay. Emerg Infect Dis 7:739-741.
4. Ostlund EN, Crom RL, Pedersen DD, et al.: 2001, Equine West Nile encephalitis, United States. Emerg Infect Dis 7:665-669.
5. Roux KH: 1995, Optimization and troubleshooting in PCR. In: PCR primer, a laboratory manual, ed. Dieffenbach CW, Dveksler GS, pp. 53-62. Cold Spring Harbor Laboratory Press, Plainview, NY.
6. Sahu SP, Alstad AD, Pedersen DD, Pearson JE: 1994, Diagnosis of eastern equine encephalomyelitis virus infection in horses by immunoglobulin M and G capture enzyme-linked immunosorbent assay. J Vet Diagn Investig 6:34-38.
7. Weaver SC, Hagenbaugh A, Bellew LA, et al.: 1993, A comparison of the nucleotide sequences of eastern and western equine encephalomyelitis viruses with those of other alphaviruses and related RNA viruses. Virology 197:375-390.