Efficient Extraction of Viral DNA and Viral RNA by the Chemagic Viral DNA/RNA Kit Allows Sensitive Detection of Cytomegalovirus, Hepatitis B Virus, and Hepatitis G Virus by PCR

Journal of Clinical Microbiology

Volumn 41, Issue 11, 2003, Pages 5273-5276

  Kleines, Michael ^a   Schellenberg, Kirsten ^a   Ritter, Klaus ^a  

^a UNIVERSITY HOSPITAL RWTH AACHEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS DNA; VIRUS RNA;

ARTICLE; CYTOMEGALOVIRUS; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; DNA DETERMINATION; DNA EXTRACTION; EVALUATION; HEPATITIS B VIRUS; HEPATITIS G VIRUS; INTERMETHOD COMPARISON; LABORATORY AUTOMATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REFERENCE VALUE; TECHNOLOGY;

CYTOMEGALOVIRUS; DNA, VIRAL; GB VIRUS C; GENE AMPLIFICATION; GENOME, VIRAL; HEPATITIS B VIRUS; HUMANS; PLASMIDS; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; RNA, VIRAL; SENSITIVITY AND SPECIFICITY;

CUCUMBER MOSAIC VIRUS; CYTOMEGALOVIRUS; DNA VIRUSES; GB VIRUS C/HEPATITIS G VIRUS; HEPATITIS B VIRUS; RNA VIRUSES;

EID: 0242425721     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.41.11.5273-5276.2003     Document Type: Article

References (6)

1. Fahle, G. A., and S. H. Fischer. 2000. Comparison of six commercial DNA extraction kits for recovery of cytomegalovirus DNA from spiked human specimens. J. Clin. Microbiol. 38:3860-3863.
2. Häusler, M., S. Scheithauer, K. Ritter, and M. Kleines. 2003. Molecular diagnosis of Epstein-Barr virus. Expert Rev. Mol. Diagn. 3:89-100.
3. Heermann, K. H., Y. Hagos, and R. Thomssen. 1994. Liquid-phase hybridization and capture of hepatitis B virus DNA with magnetic beads and fluorescence detection of PCR product. J. Virol. Methods 50:43-57.
4. Kleines, M., K. Ritter, and L. Schaade. 2002. Advances in quantification of cytomegalovirus (HCMV)-loads by real time PCR approaches. Recent Res. Devel. Microbiol. 6:107-114.
5. Legler, T. J., M. Kohle, and K. H. Heermann. 1999. High-throughput extraction, amplification, and detection (HEAD) of HCV-RNA in individual blood donations, J. Clin. Virol. 13:95-103.
6. Schaade, L., P. Kockelkorn, K. Ritter, and M. Kleines. 2000. Detection of cytomegalovirus DNA in human specimens by LightCycler PCR. J. Clin. Microbiol. 38:4006-4009.