Down-regulation of αv/β3 integrin via misrouting to lysosomes by overexpression of a β3Lamp1 fusion protein

Biochemical Journal

Volumn 370, Issue 2, 2003, Pages 703-711

  Conesa, Magali ^a   Prat, Annik ^b   Mort, John S ^c   Marvaldi, Jacques ^a   Lissitzky, Jean Claude ^a   Seidah, Nabil G ^b  

^a CNRS   (France)

^b CLIN RESEARCH INSTITUTE OF MONTREAL   (Canada)

^c MCGILL UNIVERSITY   (Canada)

Author keywords

Cellular mis sorting; Dominant negative; Lysosomal degradation

Indexed keywords

ADHESION; CELLS; MEMBRANES; PROTEINS;

HETERODIMERS; LYSOSOMAL PROTEINS;

BIOCHEMISTRY;

ALPHA5 INTEGRIN; BETA1 INTEGRIN; BETA3 INTEGRIN; DIMER; HYBRID PROTEIN; PROTEIN; PROTEOME; VITRONECTIN;

ARTICLE; CELL AGGREGATION; CHIMERA; CONTROLLED STUDY; DATA ANALYSIS; DOWN REGULATION; ENDOPLASMIC RETICULUM; ENDOSOME; HUMAN; HUMAN CELL; LYSOSOME; MEMBRANE BINDING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; REDUCTION;

ANTIGENS, CD; DOWN-REGULATION; GOLGI APPARATUS; HUMANS; INTEGRIN ALPHAV; INTEGRIN ALPHAVBETA3; INTEGRIN BETA3; LYSOSOMAL-ASSOCIATED MEMBRANE PROTEIN 1; LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEINS; LYSOSOMES; RECOMBINANT FUSION PROTEINS;

EID: 0242417643     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20021301     Document Type: Article

References (35)

1. Aguzzi, A, Brandner, S., Marino, S. and Steinbach, J. P. (1996) Transgenic and knockout mice in the study of neurodegenerative diseases. J. Mol. Med. 74, 111-126
2. Famulok, M. and Jenne, A. (1998) Oligonucleotide libraries-variatio delectat. Curr Opin. Chem. Biol. 2, 320-327
3. Rosenberg, M. P. (1997) Gene knockout and transgenic technologies in risk assessment: the next generation. Mol. Carcinog. 20, 262-274
4. Maher, L. J., III (1996) Prospects for the therapeutic use of antigene oligonucleotides. Cancer Invest 14, 66-82
5. Alama, A., Barbieri, F., Cagnoli, M. and Schettini, G. (1997) Antisense oligonucleotides as therapeutic agents. Pharmacol. Res. 36, 171-178
6. Carthew, R. W. (2001) Gene silencing by double-stranded RNA. Curr. Opin. Cell Biol. 13, 244-248
7. Lavrovsky, Y., Chen, S. and Roy, A. K. (1997) Therapeutic potential and mechanism of action of oligonucleotides and ribozymes. Biochem. Mol. Med. 62, 11-22
8. Wagner, R. W. (1995) The state of the art in antisense research. Nat. Med. 1, 1116-1118
9. Werstuck, G. and Green, M. R. (1998) Controlling gene expression in living cells through small molecule-RNA interactions. Science (Washington, D.C.) 282, 296-298
10. Herskowitz, I. (1987) Functional inactivation of genes by dominant negative mutations. Nature (London) 329, 219-222
11. Onishi, M. (1998) Usage of activating mutations in the analysis of cytokine signal transduction pathways. Int. J. Hematol. 67, 221-227
12. Smith, L. M. and Birrer, M. J. (1996) Use of transcription factors as agents and targets for drug development. Oncology 10, 1532-1538
13. Lin, X., Dashti, A., Schinazi, R. F. and Tang, J. (1993) Intracellular diversion of glycoprotein GP160 of human immunodeficiency virus to lysosomes as a strategy of AIDS gene therapy. FASEB J. 7, 1070-1080
14. Rovere, C., Mort, J. S., Chretien, M. and Seidah, N. G. (2000) Cathepsin-B fusion proteins misroute secretory protein partners such as the proprotein convertase PC2-7B2 complex toward the lysosomal degradation pathways. Biochem. Biophys. Res. Commun. 276, 594-599
15. Li, F. Q., Coonrod, A. and Horwitz, M. (1996) Preferential MyoD homodimer formation demonstrated by a general method of dominant negative mutation employing fusion with a lysosomal protease. J. Cell Biol. 135, 1043-1057
16. Rohrer, J., Schweizer, A., Russell, D. and Kornfeld, S. (1996) The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane. J. Cell Biol. 132, 565-576
17. Ruoslahti, E. (1996) RGD and other recognition sequences for integrins. Annu. Rev. Cell Dev. Biol. 12, 697-715
18. Mach, L., Schwihla, H., Stüwe, K., Rowan, A. D., Mort, J. S. and Glössl, J. (1993) Activation of procathepsin B in human hepatoma cells: the conversion to the mature enzyme relies on the action of cathepsin B itself. Biochem. J. 293, 437-442
19. Lehmann, M., Rabenandrasana, C., Tamura, R., Lissitzky, J. C., Quaranta, V., Pichon, J. and Marvaldi, J. (1994) A monoclonal antibody inhibits adhesion to fibronectin and vitronectin of a colon carcinoma cell line and recognizes the integrins alpha v beta 3, alpha v beta 5, and alpha v beta 6. Cancer Res. 54, 2102-2107
20. Kieffer, N., Fitzgerald, L. A., Wolf, D., Cheresh, D. A. and Phillips, D. R. (1991) Adhesive properties of the beta 3 integrins: comparison of GP IIb-IIIa and the vitronectin receptor individually expressed in human melanoma cells. J. Cell Biol. 113, 451-461
21. Felding-Habermann, B. and Cheresh, D. A. (1993) Vitronectin and its receptors. Curr. Opin. Cell Biol. 5, 864-868
22. Xiong, J. P., Stehle, T., Zhang, R., Joachimiak, A., Frech, M., Goodman, S. L. and Arnaout, M. A. (2002) Crystal structure of the extracellular segment of integrin αVβ3 in complex with an Arg-Gly-Asp ligand. Science (Washington, D.C.) 296, 151-155
23. Lippincott-Schwartz, J., Yuan, L., Tipper, C., Amherdt, M., Orci, L. and Klausner, R. D. (1991) Brefeldin A's effects on endosomes, lysosomes, and the TGN suggest a general mechanism for regulating organelle structure and membrane traffic. Cell (Cambridge, Mass) 67, 601-616
24. Lehmann, M., Rigot, V., Seidah, N. G., Marvaldi, J. and Lissitzky, J. C. (1996) Lack of integrin alpha-chain endoproteolytic cleavage in furin-deficient human colon adenocarcinoma cells LoVo. Biochem. J. 317, 803-809
25. Barile, F. A., Guzowski, D. E., Ripley, C., Siddiqi, Z. A. and Bienkowski, R. S. (1990) Ammonium chloride inhibits basal degradation of newly synthesized collagen in human fetal lung fibroblasts. Arch. Biochem. Biophys. 276, 125-131
26. 4Cl. Eur. J. Biochem. 191, 591-597
27. Smith, J. W. and Cheresh, D. A. (1990) Integrin (αVβ3)-ligand interaction. Identification of a heterodimeric RGD binding site on the vitronectin receptor. J. Biol. Chem. 265, 2168-2172
28. Sonnenberg, A., Modderman, P. W. and Hogervorst, F. (1988) Laminin receptor on platelets is the integrin VLA-6. Nature 336, 487-489
29. Lee, E. C., Lotz, M. M., Steele, Jr, G. D. and Mercuric, A. M. (1992) The integrin α 6β4 is a laminin receptor. J. Cell Biol. 117, 671-678
30. Gimond, C., van Der Flier, A., van Delft, S., Brakebusch, C., Kuikman, I., Collard, J. G., Fassler, R. and Sonnenberg, A. (1999) Induction of cell scattering by expression of beta1 integrins in beta1-deficient epithelial cells requires activation of members of the rho family of GTPases and downregulation of cadherin and catenin function. J. Cell Biol. 147, 1325-1340
31. Simon, K. O., Nutt, E. M., Abraham, D. G., Rodan, G. A. and Duong, L. T. (1997) The αVβ3 integrin regulates α5β1-mediated cell migration toward fibronectin. J. Biol. Chem. 272, 29380-29389
32. Honda, S., Tomiyama, Y., Pelletier, A. J., Annis, D., Honda, Y., Orchekowski, R., Ruggeri, Z. and Kunicki, T. J. (1995) Topography of ligand-induced binding sites, including a novel cation-sensitive epitope (AP5) at the amino terminus, of the human integrin beta 3 subunit. J. Biol. Chem. 270, 11947-11954
33. Schaffner-Reckinger, E., Gouon, V., Melchior, C., Plancon, S. and Kieffer, N. (1998) Distinct involvement of β3 integrin cytoplasmic domain tyrosine residues 747 and 759 in integrin-mediated cytoskeletal assembly and phosphotyrosine signaling. J. Biol. Chem. 273, 12623-12632
34. Peyruchaud, O., Nurden, A. T., Milet, S., Macchi, L., Pannochia, A., Bray, P. F., Kieffer, N. and Bourre, F. (1998) R to Q amino acid substitution in the GFFKR sequence of the cytoplasmic domain of the integrin IIb subunit in a patient with a Glanzmann's thrombasthenia-like syndrome. Blood 92, 4178-4187
35. Hotchin, N. A., Gandarillas, A. and Watt, F. M. (1995) Regulation of cell surface β1 integrin levels during keratinocyte terminal differentiation. J. Cell Biol. 128, 1209-1219