Detection of malaria sporozoites by standard ELISA and VecTest™ dipstick assay in field-collected anopheline mosquitoes from a malaria endemic site in Ghana

Tropical Medicine and International Health

Volumn 8, Issue 11, 2003, Pages 1012-1017

  Appawu, Maxwell A ^a   Bosompem, Kwabena M ^a   Dadzie, Samuel ^a   McKakpo, Uri S ^a   Anim Baidoo, Isaac ^a   Dykstra, Elizabeth ^b   Szumlas, Daniel E ^b   Rogers, William O ^a,b   Koram, Kwadwo ^a   Fryauff, David J ^c  

^a University of Ghana   (Ghana)

^b US Naval Medical Research Unit No 3 Egypt   (Egypt)

^c NAVAL MEDICAL RESEARCH CENTER   (United States)

Author keywords

Anopheles; Circumsporozoite protein; Dipstick assay; Malaria vectors; Plasmodium falciparum

Indexed keywords

CIRCUMSPOROZOITE PROTEIN; PROTOZOAL PROTEIN; UNCLASSIFIED DRUG;

BIOASSAY; DETECTION METHOD; HEALTH MONITORING; MALARIA; MOSQUITO;

ANOPHELES; ANOPHELES FUNESTUS; ANOPHELES GAMBIAE; ARTICLE; CHROMATOGRAPHY; CIRCUMSPOROZOITE MICROPLATE ENZYME LINKED IMMUNOSORBENT ASSAY; CORRELATION ANALYSIS; DIAGNOSTIC ACCURACY; ENDEMIC DISEASE; ENZYME LINKED IMMUNOSORBENT ASSAY; GHANA; INTERMETHOD COMPARISON; MALARIA; MOSQUITO; NONHUMAN; PARASITE VECTOR; PLASMODIUM; PLASMODIUM FALCIPARUM; PREVALENCE; REFERENCE VALUE; SENSITIVITY AND SPECIFICITY; SPOROZOITE; STATISTICAL ANALYSIS;

ANIMALS; ANOPHELES; ENDEMIC DISEASES; ENZYME-LINKED IMMUNOSORBENT ASSAY; GHANA; HUMANS; INSECT VECTORS; MALARIA, FALCIPARUM; PLASMODIUM FALCIPARUM; PREVALENCE; RANDOM ALLOCATION; REAGENT KITS, DIAGNOSTIC; SENSITIVITY AND SPECIFICITY; SPOROZOITES;

GHANA;

ANOPHELES FUNESTUS; ANOPHELES GAMBIAE; PLASMODIUM FALCIPARUM;

EID: 0242408550     PISSN: 13602276     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1360-2276.2003.00127.x     Document Type: Article

References (9)

1. Beier JC & Koros JK (1991) Visual assessment of sporozoite and blood meal ELISA samples in malaria field studies. Journal of Medical Entomology 28, 805-808.
2. Bockarie WJ, Jawara M & Banish G (1993) Visual assessment of sporozoite ELISA results is as reliable as a plate reader in determining infection rates in field samples of Anopheles gambiae s.s. Transactions of the Royal Society of Tropical Medicine and Hygiene 87, 602.
3. Collins FH, Procell PM, Campbell GH & Collins WE (1988) Monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA) for detection of Plasmodium malariae sporozoites in mosquitoes. American Journal of Tropical Medicine and Hygiene 38, 283-288
4. Gilles MT & De Meillon B (1968) The Anophelinae of Africa South of Sahara Ethiopian Zoogeographical Region, 2nd edn, no. 54. The South African Institute for Medical Research, Johannesburg, 343 pp.
5. Ryan JR, Dave K, Emmerich E et al. (2001) Dipstick for rapid detection of Plasmodium in vectoring Anopheles mosquitoes. Medical and Veterinary Entomology 15, 225-230.
6. Ryan JR, Dave K, Collins KM et al. (2002) Extensive multiple test centre evaluation of the VecTest malaria antigen panel assay. Medical and Veterinary Entomology 16, 321-327.
7. Stauffer WM & Kamat D (2003) Special challenges in the prevention and treatment of malaria in children. Current Infectious Diseases Report 5, 43-52.
8. Wittz RA, Zavala F, Charoenvit Y, Campbell GH & Burkot TR (1987) Comparative testing of Plasmodium falciparum sporozoite monoclonal antibodies for ELISA development. Bulletin of World Health Organization 65, 39-45.
9. World Health Organization (WHO) (1975) Manual on Practical Entomology in Malaria. Part II. Methods and Techniques 13. World Health Organization, Geneva.