HPLC analyses of seed storage proteins reveal polymorphism in Italian common bean (Phaseolus vulgaris L.) ecotypes

Euphytica

Volumn 134, Issue 1, 2003, Pages 85-95

  Mennella, Giuseppe ^a   Onofaro Sanajà, Vincenzo ^a   D'Alessandro, Antonietta ^a   Milone, Marco ^a   Perrone, Domenico ^a  

^a Centro di Ricerca per l'Orticoltura   (Italy)

Author keywords

AE HPLC; Common bean; Germplasm; Phaseolin; Phytohemagglutinin

Indexed keywords

EMBRYOPHYTA; PHASEOLUS (ANGIOSPERM); PHASEOLUS VULGARIS;

EID: 0242402188     PISSN: 00142336     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1026127004224     Document Type: Article

References (27)

1. Avitabile, A., A. Pentangelo, I. Giordano, A. Di Mauro & R. Rao, 1999. Caratterizzazione bioagronomica dell'ecotipo 'Fagiolo di Controne'. Atti V Convegno Nazionale Biodiversità, 'Biodiversità e sistemi ecocompatibili', pp. 471-8. Caserta 9-10 Settembre 1999.
2. Bonetti, A., A. Miggiano, G. Dinelli & A. Lovato, 1995. Identification of bean (Phaseolus vulgaris L.) cultivars grown in Italy by field and electrophoresis tests: a comparative study. Seed Sci & Technol 23: 69-84.
3. Bradford, M., 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding. Analyt Biochem 72: 248-54.
4. Brown, J.W.S., T.C. Osborn, F.A. Bliss & T.C. Hall, 1982. Bean lectins Part 2: Relationship between qualitative lectin variation in Phaseolus vulgaris L. and previous observations on purified bean lectins. Theor Appl Genet 62: 361-7.
5. Cooke, R.J., 1995. Varietal identification of crop plants. In: J.H. Skerritt & R. Appels (Eds.), New Diagnostics in Crop Sciences, pp. 33-63, CAB INTERNATIONAL, Wallingford, UK.
6. Gepts, P., T.C. Osborn, K. Rashka & F.A. Bliss, 1986. Phaseolin protein variability in wild forms and landraces of the common bean (Phaseolus vulgaris L.): evidence for multiple centers of domestications. Econ Bot 40: 451-68.
7. Gepts, P. & F.A. Bliss, 1988. Dissemination pathways of common bean (Phaseolus vulgaris, Fabaceae) deduced from phaseolin electrophoretic variability: II Europe and Africa. Econ Bot 42: 86-104.
8. Hubert, L.J., 1987. Assignment Methods in Combinational Data Analysis. Marcell Dekker, New York, p. 326.
9. Laemmli, U.K., 1970. Cleavage of structure proteins during assembly of the head of Bacteriophage T4. Nature 227: 680-5.
10. Limongelli, G.M., G. Laghetti, P. Perrino & A.R. Piergiovanni, 1996. Variation of seed storage proteins in landraces of common bean (Phaseolus vulgaris L.) from Basilicata, Southern Italy. Euphytica 92: 393-399.
11. Lioi, L., 1989a. Variation of the storage protein phaseolin in common bean (Phaseolus vulgaris L.) from the Mediterranean area. Euphytica 44: 151-155.
12. Lioi, L., 1989b. Geographical variation of phaseolin patterns in an old world collection of Phaseolus vulgaris. Seed Sci & Technol 17: 317-324.
13. Lioi, L., 1991. Electrophoretic variation and geographical distribution of the seed protein phytohemagglutinin in cultivated Phaseolus vulgaris L. J Genet & Breed 45: 97-102.
14. Mennella, G., M. Bianchi, V. Onofaro Sanajà, A. Tonini & V. Magnifico, 1995. Caratterizzazione biochimica e morfo-fisiologica di cultivar di Brassica oleracea L. Sementi Elette 6: 9-16.
15. Mennella, G., A. Iori, V. Onofaro Sanajà & V. Magnifico, 1996. Broccoli and cauliflower cultivar identification through IE-HPLC seed protein analysis. Acta Horticulturae 407: 115-121.
16. Mennella, G., A. D'Alessandro, V. Onofaro Sanajà, & V. Magnifico, 1998. Caratterizzazione biochimica di pomodoro San Marzano mediante tecniche elettroforetiche e cromatografiche. Italus Hortus 4: 36-41.
17. Mennella, G., V. Onofaro Sanajà, A. Tonini, & V. Magnifico, 1999. Seed storage protein characterization of Solanum species and of cultivars and androgenetic lines of S. melongena L. by SDS-PAGE and AE-HPLC. Seed Sci & Technol 27: 23-35.
18. Mennella, G., V. Onofaro Sanajà, A. D'Alessandro, R. Coppola & I. Poma, 2001. Tomato ecotype characterization by anionic exchange-high performance liquid chromatography analysis of endosperm seed proteins. Acta Horticulturae 546: 453-457.
19. Osborn, T.C., M. Burow & H.A. Bliss, 1988. Purification and characterization of arcelin seed protein from common bean. Plant Physiol 86: 399-405.
20. Pustzai, A., E.M.W. Clarke, T.P. King & J.C. Stewart, 1979. Nutritional evaluation of kidney beans (Phaseolus vulgaris): chemical composition, lectin content, and nutritional value of selected cultivars. J Sci Food Agric 30: 843-848.
21. Rohlf, F.J., 1993. NTSYS-pc Numerical Taxonomy and Multivariate Analysis System. Exeter Publication, Setauker, NY, p. 326.
22. Romero Andreas, J., B.S. Yandell & F.A. Bliss, 1986. Bean arcelin I. Inheritance of a novel seed protein of Phaseolus vulgaris L. and its effect on seed composition. Theor Appl Genet 72: 123-128.
23. Singh, S.P., P. Gepts & D.G. Debouck, 1991. Races of common bean (Phaseolus vulgaris, Fabaceae). Econ Bot 45: 379-396.
24. Smith, J.A. & S.L. Desborough, 1987. The endosperm seed protein solin: biochemical characterization, induction by ABA and species-specific subunits. Theor Appl Genet 74: 739-745.
25. Smith, J.S.C. & O.S. Smith, 1992. Fingerprinting crop varieties. Adv in Agron 47: 85-140.
26. Sokal, R. & C. Michener, 1958. A statistical method for evaluating systematic relationship. Univ Kan Sci Bull 38: 1409-1438.
27. Thorne, J., Ch.O. Toro, J. Vargas & D.G. Debouck, 1995. Variability in Andean nuña common beans (Phaseolus vulgaris, Fabaceae). Econ Bot 49: 78-95.