Utility of Gram Stain for the Microbiological Analysis of Burn Wound Surfaces

Archives of Pathology and Laboratory Medicine

Volumn 127, Issue 11, 2003, Pages 1485-1488

  Elsayed, Sameer ^a,b   Gregson, Daniel B ^a,b,c   Lloyd, Tracie ^a   Crichton, Marilyn ^a   Church, Deirdre L ^a,b,c  

^a CALGARY LABORATORY SERVICES   (Canada)

^b UNIVERSITY OF CALGARY   (Canada)

^c UNIVERSITY OF CALGARY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ADOLESCENT; ADULT; AGED; ARTICLE; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BURN; CHILD; CORRELATION ANALYSIS; DIAGNOSTIC ACCURACY; GRAM STAINING; HUMAN; HUMAN TISSUE; MAJOR CLINICAL STUDY; WOUND INFECTION; CANADA; CANDIDA; ENTEROCOCCUS; GROWTH, DEVELOPMENT AND AGING; HEALTH SERVICE; HOSPITALIZATION; INFANT; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; MIDDLE AGED; PREDICTION AND FORECASTING; PRESCHOOL CHILD; PROSPECTIVE STUDY; PSEUDOMONAS; SENSITIVITY AND SPECIFICITY; STAINING; STANDARD; STAPHYLOCOCCUS; STATISTICS; STREPTOCOCCUS;

CRYSTAL VIOLET; DIAGNOSTIC AGENT; GRAM'S STAIN; PHENAZINE DERIVATIVE;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; ALBERTA; BURNS; CANDIDA; CHILD; CHILD, PRESCHOOL; ENTEROCOCCUS; GENTIAN VIOLET; HOSPITALIZATION; HUMANS; INFANT; MIDDLE AGED; PHENAZINES; PREDICTIVE VALUE OF TESTS; PROSPECTIVE STUDIES; PSEUDOMONAS; SENSITIVITY AND SPECIFICITY; STAINING AND LABELING; STAPHYLOCOCCUS; STREPTOCOCCUS; URBAN HEALTH SERVICES;

EID: 0242335686     PISSN: 00039985     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (22)

1. Dodd D, Stutman HR. Current issues in burn wound infections. Adv Pediatr Infect Dis. 1991;6:137-162.
2. Edlich RF, Rodeheaver GT, Spengler M, Hiebert J, Edgerton MT. Practical bacteriologic monitoring of the burn victim. Clin Plast Surg. 1977;4:561-569.
3. Papini RP, Wilson AP, Steer JA, McGrouther DA, Parkhouse N. Wound management in burn centres in the United Kingdom. Br J Surg. 1995;82:505-509.
4. Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH, eds. Manual of Clinical Microbiology. 7th ed. Washington, DC: American Society for Microbiology; 1999.
5. Pruitt BA Jr, McManus AT, Kim SH, Goodwin CW. Burn wound infections: current status. World J Surg. 1998;22:135-145.
6. Mason AD, McManus AT, Pruitt BA. Association of burn mortality and bacteremia. Arch Surg. 1986;121:1027-1031.
7. Peck MD, Heimbach DM. Does early excision of burn wounds change the pattern of mortality? J Burn Care Rehabil. 1989;10:7-10.
8. McManus AT, Mason AD, McManus WF, Pruitt BA Jr. A decade of reduced Gram-negative infections and mortality associated with improved isolation of burned patients. Arch Surg. 1994;129:1306-1309.
9. Smith DJ Jr, Thomson PD. Changing flora in burn and trauma units: historical perspective: experience in the United States. J Burn Care Rehabil. 1992;13(2 pt 2):276-280.
10. Weber JM, Tompkins DM. Improving survival: infection control and burns. AACN Clin Issues Crit Care Nurs. 1993;4:414-423.
11. Weber JM, Sheridan RL, Pasternack MS, Tompkins RG. Nosocomial infections in pediatric patients with burns. Am J Infect Control. 1997;25:195-201.
12. Woolfrey BF, Fox JM, Quall CO. An evaluation of burn wound quantitative microbiology, I: quantitative eschar cultures. Am J Clin Pathol. 1981;75:532-537.
13. Karyoute SM. Burn wound infection in 100 patients treated in the burn unit at Jordan University Hospital. Burns. 1989;15:117-119.
14. Mousa HAL. Aerobic, anaerobic and fungal burn wound infections. J Hosp Infect. 1997;37:317-323.
15. Rodgers GL, Mortensen J, Fisher MC, Lo A, Cresswell A, Long SS. Predictors of infectious complications after burn injuries in children. Pediatr Infect Dis J. 2000;19:990-995.
16. Taddonio TE, Thomson PD, Tait MJ, Prasad JK, Feller I. Rapid quantification of bacterial and fungal growth in burn wounds: biopsy homogenate Gram stain versus microbial culture results. Burns. 1988;14:180-184.
17. Cook N. Methicillin-resistant Staphylococcus aureus versus the burn patient. Burns. 1998;24:91-98.
18. Falk PS, Winnike J, Woodmansee C, Desai M, Mayhall CG. Outbreak of vancomycin-resistant enterococci in a burn unit. Infect Control Hosp Epidemiol. 2000;21:575-582.
19. Freshwater MF, Su CT. Potential pitfalls of quantitative burn wound biopsy cultures. Ann Plas Surg. 1980;4:216-218.
20. McManus AT, Kim SH, McManus WF, Mason AD, Pruitt BA Jr. Comparison of quantitative microbiology and histopathology in divided burn-wound biopsy specimens. Arch Surg. 1987;122:74-76.
21. Levine NS, Lindberg RC, Mason AD Jr, Pruitt BA Jr. The quantitative swab culture and smear: a quick, simple method for determining the number of viable aerobic bacteria on open wounds. J Trauma. 1976;16:89-94.
22. Tahlan RN, Keswani RK, Saini S, Miglani OP. Correlation of quantitative burn wound biopsy culture and surface swab culture to burn wound sepsis. Burns Incl Therm Inj. 1984;10:217-224.