The N- and C-Terminal Regions of Rotavirus NSP5 Are the Critical Determinants for the Formation of Viroplasm-Like Structures Independent of NSP2

Journal of Virology

Volumn 77, Issue 22, 2003, Pages 12184-12192

  Mohan, K V K ^a   Muller, J ^a   Atreya, C D ^a,b  

^a CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

^b NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; MESSENGER RNA; NSP2 PROTEIN; NSP5 PROTEIN; UNCLASSIFIED DRUG; VIRUS PROTEIN; VIRUS RNA;

ARTICLE; GASTROENTERITIS; GENE EXPRESSION; GENETIC TRANSFECTION; IMMUNOBLOTTING; IMMUNOFLUORESCENCE; IMMUNOPRECIPITATION; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; ROTAVIRUS; TRANSMISSION ELECTRON MICROSCOPY; VIRUS INFECTION;

ANIMALS; COS CELLS; CYTOPLASM; MICROSCOPY, ELECTRON; PHOSPHORYLATION; RNA-BINDING PROTEINS; ROTAVIRUS; TRANSFECTION; VIRAL NONSTRUCTURAL PROTEINS; VIRAL PROTEINS; VIRUS ASSEMBLY;

EID: 0242300179     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.77.22.12184-12192.2003     Document Type: Article

References (23)

1. Afrikanova, I., M. C. Miozzo, S. Giambiagi, and O. R. Burrone. 1996. Phosphorylation generates different forms of rotavirus NSP5. J. Gen. Virol. 77:2059-2065.
2. Afrikanova, I., E. Fabbretti, M. C. Miozzo, and O. R. Burrone. 1998. Rotavirus NSP5 phosphorylation is upregulated by interaction with NSP2. J. Gen. Virol. 79:2679-2686.
3. Berois, M., C. Sapin, I. Erk, D. Poncet, and J. Cohen. 2003. Rotavirus nonstructural protein NSP5 interacts with major core protein VP2. J. Virol. 77:1757-1763.
4. Blackhall, J., A. Fuentes, K. Hansen, and G. Magnusson. 1997. Serine protein kinase activity associated with rotavirus phosphoprotein NSP5. J. Virol. 71:138-144.
5. Blackhall, J., M. Munoz, A. Fuentes, and G. Magnusson. 1998. Analysis of rotavirus nonstructural protein NSP5 phosphorylation. J. Virol. 72:6398-6405.
6. Chnaiderman, J., M. Barro, and E. Spencer. 2002. NSP5 phosphorylation regulates the fate of viral mRNA in rotavirus-infected cells. Arch. Virol. 147:1899-1911.
7. Dector, M. A., P. Romero, S. Lopez, and C. F. Arias. 2002. Rotavirus gene silencing by small interfering RNAs. EMBO Rep. 3:1175-1180.
8. Eichwald, C., F. Vascotto, E. Fabbretti, and O. R. Burrone. 2002. Rotavirus NSP5: mapping phosphorylation sites and kinase activation and viroplasm localization domains. J. Virol. 76:3461-3470.
9. Estes, M. K. 2001. Rotaviruses and their replication, p. 1747-1785. In D. M. Knipe, P. M. Howley, D. E. Griffin, R. A. Lamb, M. A. Martin, B. Roizman, and S. E. Straus (ed.), Fields virology, 4th ed. Lippincott Williams & Wilkins, Philadelphia, Pa.
10. Estes, M. K., E. L. Pamer, and J. F. Obijeski. 1983. Rotaviruses: a review. Curr. Top. Microbiol. Immunol. 105:123-184.
11. Fabbretti, E., I. Afrikanova, F. Vascotto, and O. R. Burrone. 1999. Two non-structural rotaviral proteins, NSP2 and NSP5, form viroplasm-like structures in vivo. J. Gen. Virol. 80:333-339.
12. Gonzalez, R. A., M. A. Torres-Vega, S. Lopez, and C. F. Arias. 1998. In vivo interactions among rotavirus nonstructural proteins. Arch. Virol. 143:981-996. (Erratum, 143:2064.)
13. Gonzalez, S. A., and O. R. Burrone. 1991. Rotavirus NS26 is modified by addition of single O-linked residues of N-acetylglucosamine. Virology 182: 8-16.
14. Kapikian, A. Z., Y. Hoshino, and R. M. Chanock. 2001. Rotaviruses, p. 1787-1833. In D. M. Knipe, P. M. Howley, D. E. Griffin, R. A. Lamb, M. A. Martin, B. Roizman, and S. E. Straus (ed.), Fields virology, 4th ed. Lippincott Williams & Wilkins, Philadelphia, Pa.
15. Mohan, K. V., and C. D. Atreya. 2000. Comparative sequence analysis identified mutations outside the NSP4 cytotoxic domain of tissue culture-adapted ATCC-Wa strain of human rotavirus and a novel inter-species variable domain in its C-terminus. Arch. Virol. 145:1789-1799.
16. Mohan, K. V., T. S. Dermody, and C. D. Atreya. 2000. Mutations selected in rotavirus enterotoxin NSP4 depend on the context of its expression. Virology 275:125-132.
17. Mohan, K. V., and C. D. Atreya. 2001. Nucleotide sequence analysis of rotavirus gene 11 from two tissue culture-adapted ATCC strains, RRV and Wa. Virus Genes 23:321-329.
18. Mohan, K. V., I. Som, and C. D. Atreya. 2002. Identification of a peroxisomal targeting signal 1 (PTS1)-containing viral protein and its targeting to peroxisomes. J. Virol. 76:2543-2547.
19. Petrie, B. L., H. B. Greenberg, D. Y. Graham, and M. K. Estes. 1984. Ultrastructural localization of rotavirus antigens using colloidal gold. Virus Res. 1:133-152.
20. Poncet, D., P. Lindenbaum, R. l'Haridon, and J. Cohen. 1997. In vivo and in vitro phosphorylation of rotavirus NSP5 correlates with its localization in viroplasms. J. Virol. 71:34-41.
21. Torres-Vega, M. A., R. A. Gonzalez, M. Duarte, D. Poncet, S. Lopez, and C. F. Arias. 2000. The C-terminal domain of rotavirus NSP5 is essential for its multimerization, hyperphosphorylation and interaction with NSP6. J. Gen. Virol. 81:821-830.
22. Vende, P., Z. F. Taraporewala, and J. T. Patton. 2002. RNA-binding activity of the rotavirus phosphoprotein NSP5 includes affinity for double-stranded RNA. J. Virol. 76:5291-5299.
23. Welch, S. K., S. E. Crawford, and M. K. Estes. 1989. Rotavirus SA11 genome segment 11 protein is a nonstructural phosphoprotein. J. Virol. 63:3974-3982.