One Step Purification and Biochemical Characterization of a Spermatozoa-Binding Protein From Porcine Oviductal Epithelial Cells

Molecular Reproduction and Development

Volumn 66, Issue 4, 2003, Pages 383-390

  Marini, Patricia E ^a   Cabada, Marcelo O ^a,b  

^a Instituto de Biologia Molecular y Celular de Rosario   (Argentina)

^b NONE   (Argentina)

Author keywords

Glycoprotein; Oviduct; Pig; Sperm; Sperm reservoir

Indexed keywords

BINDING PROTEIN; CARBOHYDRATE; LECTIN; MERCAPTOETHANOL; SERINE; SPERMATOZOA BINDING PROTEIN; THREONINE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; CONJUGATION; CONTROLLED STUDY; DEGLYCOSYLATION; ENZYMATIC DEGRADATION; ENZYME ACTIVITY; ENZYME ASSAY; FEMALE; ISOELECTRIC FOCUSING; LECTIN BINDING; MALE; MOLECULAR WEIGHT; NONHUMAN; OVIDUCT; PERIODIC ACID SCHIFF STAIN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DETERMINATION; PROTEIN INTERACTION; PROTEIN PURIFICATION; REDUCTION; SILVER STAINING; SPERM; SWINE; UTERINE TUBE MUCOSA;

ANIMALS; CARBOHYDRATE METABOLISM; CARRIER PROTEINS; CHROMATOGRAPHY, AFFINITY; EPITHELIAL CELLS; FEMALE; FLUORESCEIN-5-ISOTHIOCYANATE; GLYCOPROTEINS; MALE; OVIDUCTS; SPERMATOZOA; SWINE;

SUIDAE; SUS SCROFA;

EID: 0242290382     PISSN: 1040452X     EISSN: None     Source Type: Journal    
DOI: 10.1002/mrd.10361     Document Type: Article

References (40)

1. Arranz S, Cabada MO. 2000. Difusible highly glycosylated protein from Bufo arenarum egg-jelly coat: Biological activity. Mol Reprod Dev 56:392-400.
2. Baillie HS, Pacey AA, Warren MA, Scudamore IW, Barrat CLR. 1997. Greater numbers of human spermatozoa associate with endosalpingeal cells derived from the isthmus compared with those from the ampulla. Human Reprod 12:1985-1992.
3. Barisone GA, Hedrick JL, Cabada MO. 2002. Vitelline envelope of Bufo arenarum: Biochemical and biological characterization. Biol Reprod 66:1203-1209.
4. Berger T, Turner KO, Meizel S, Hedrick JL. 1989. Zona pellucida-induced acrosome reaction in boar sperm. Biol Reprod 40:525-530.
5. Calvete JJ, Carrera E, Sanz L, Töpfer-Petersen E. 1996. Boar spermadhesins AQN-1 and AQN-3: Oligosaccharide and zona pellucida binding characteristics. Biol Chem 377:521-527.
6. DeMott RP, Lefebvre R, Suarez SS. 1995. Carbohydrates mediate the adherence of hamster sperm to oviductal epithelium. Biol Reprod 52:1395-1403.
7. Dobrinsky I, Smith T, Suarez S, Ball B. 1997. Membrane contact with oviductal epithelium modulates the intracellular calcium concentration of equine spermatozoa in vitro. Biol Reprod 56:861-869.
8. Dostalova Z, Calvete JJ, Sanz L, Töpfer-Petersen E. 1995. Boar spermadhesin AWN-1. Oligosaccharide and zona pellucida binding characteristics. Eur J Biochem 230:329-336.
9. Fazeli A, Duncan AE, Watson PF, Holt VW. 1999. Sperm-oviduct interaction: Induction of capacitation and preferential binding of uncapacitated spermatozoa to oviductal epithelial cells in porcine species. Biol Reprod 60:869-886.
10. Flesch F, Gadella B. 2000. Dynamics of the mammalian sperm plasma membrane in the process of fertilization. Biochim Biophys Acta 1469:197-235.
11. Geng J, Raub T, Baker C, Sawada G, Ma L, Elhammer A. 1997. Expression of a P-selectin ligand in zona pellucida of porcine oocytes and P-selectin on acrosomal membrane of porcine sperm cells. Potential implications for their involvement in sperm-egg interactions. J Cell Biol 137:743-754.
12. Gradilone SA, Arranz S, Cabada MO. 1998. Detection of highly glycosylated proteins in polyacrylamide gels. Anal Biochem 261:224-227.
13. Green CE, Bredl J, Holt WV, Watson PF, Fazeli A. 2001. Carbohydrate mediation of boar sperm binding to oviductal epithelial cells in vitro. Reproduction 122:305-315.
14. Gualtieri R, Talevi R. 2000. In vitro cultured bovine oviductal cells bind acrosome-intact sperm and retain this ability upon sperm release. Biol Reprod 62:1754-1762.
15. Harper MJK. 1994. Gamete and zygote transport. In: Knobil E, Neill JD, editors. The physiology of reproduction. New York: Raven Press. pp 123-187.
16. Hunter RHF. 1981. Sperm transport and reservoirs in the pig oviduct in relation to the time of ovulation. J Reprod Fertil 63:109-117.
17. Hunter RHF, Nichol R. 1983. Transport of spermatozoa in the sheep oviduct: Preovulatory sequestering of cells in the caudal isthmus. J Exp Zool 228:121-128.
18. Hunter RHF, Flechon B, Flechon JE. 1991. Distribution, morphology, and epithelial interactions of bovine spermatozoa in the oviduct before and after ovulation: A scanning electron microscopy study. Tissue Cell 23:641-656.
19. Ignotz G, Lo M, Perez C, Gwathmey T, Suarez S. 2001. Characterization of a fucose-binding from bull sperm and seminal plasma that may be responsible for formation of the oviduct sperm reservoir. Biol Reprod 64:1806-1811.
20. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
21. Lefebvre R, Chenoweth P, Drost M, LeClear C, MacCubbin M, Dutton J, Suarez S. 1995. Characterization of the oviductal sperm reservoir in cattle. Biol Reprod 53:1066-1074.
22. Lefebvre R, Lo MC, Suarez SS. 1997. Bovine sperm binding to oviductal epithelium involves fucose recognition. Biol Reprod 56:1198-1204.
23. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the Folin phenol reagent. J Biol Chem 193:265-275.
24. Martinez ML, Martelotto L, Cabada MO. 2000. Purification and biological characterization of N-acetyl β-D glucosaminidase from Bufo arenarum spermatozoa. Mol Reprod Dev 57:194-203.
25. Miceli DC, Cabada MO. 1998. Amphibian fertilization. Trends Comp Biochem Physiol 5:249-265.
26. Olympus Optical Company, Ltd. 2000. The use of the olympus fluorescence microscope. Tokyo, Japan.
27. Ozols J. 1990. Preparation of membrane fractions. In: Methods in enzymology - guide to protein purification. California: Academic Press. pp 225-235.
28. Raychoudhury S, Suarez S, Buhi W. 1993. Distribution of lectin binding sites in the oviducts of cycling and hormone-treated pigs. J Exp Zool 265:659-668.
29. Rodriguez-Martinez H, Tienthai P, Suzuki K, Funahashi H, Ekwall H, Johannisson A. 2001. Involvement of oviduct in sperm capacitation and oocyte development in pigs. Reprod Suppl 58:129-145.
30. Scott M. 2000. A glimpse at sperm function in vivo: Sperm transport and epithelial interaction in the female reproductive tract. Anim Reprod Sci 60-61:337-348.
31. Smith TT, Yanagimachi R. 1991. Attachment and release of spermatozoa from the caudal isthmus of the hamster oviduct. J Reprod Fertil 91:567-573.
32. Suarez SS. 1987. Sperm transport and motility in the mouse oviduct: Observations in situ. Biol Reprod 36:203-210.
33. Suarez SS. 1998. Minireview. The oviductal sperm reservoir in mammals: Mechanisms of formation. Biol Reprod 58:1105-1107.
34. Suarez SS. 2002. Formation of a reservoir of sperm in the oviduct. Reprod Dom Anim 37:140-143.
35. Suarez SS, Brockman K, Lefebvre R. 1997. Distribution of mucus and sperm in bovine oviducts after artificial insemination. Biol Reprod 56:447-453.
36. Töpfer-Petersen E. 1999. Molecules on the sperm route to fertilization. J Exp Zool 285:259-266.
37. Töpfer-Petersen E, Wagner A, Friedrich J, Petrunkina A, Ekhlasi-Hundrieser M, Waberski D, Drommer W. 2002. Function of the mammalian oviductal sperm reservoir. J Exp Zool 292:210-215.
38. Valz-Gianinet JN, del Pino EJ, Cabada MO. 1991. Glycoproteins from Bufo arenarum vitelline envelope with fertility-impairing effect on homologous spermatozoa. Dev Biol 146:416-422.
39. Van den Steen P, Rudd P, Dwek R, Opdenakker G. 1998. Concepts and principles of O-linked glycosylation. Crit Rev Biochem Mol Biol 33:151-208.
40. Wagner A, Ekhlasi-Hundrieser M, Hettel C, Petrunkina A, Waberski D, Nimtz M, Töpfer-Petersen E. 2002. Carbohydrate-based interactions of oviductal sperm reservoir formation-studies in the pig. Mol Reprod Dev 61:249-257.