Rapid monitoring of autolysis process of proteases by capillary electrophoresis

Biotechnology Letters

Volumn 25, Issue 20, 2003, Pages 1763-1767

  Chen, Xiu Lan ^a   Shun, Cai Yun ^a   Zhang, Yu Zhong ^a   Gao, Pei Ji ^a  

^a SHANDONG UNIVERSITY   (China)

Author keywords

Autolysis; Capillary electrophoresis; Protease

Indexed keywords

PROTEASE MCP 01, PSEUDOALTERMONAS; PROTEASE MCP-01, PSEUDOALTERMONAS; PROTEINASE;

ARTICLE; AUTOLYSIS; CAPILLARY ELECTROPHORESIS; CHEMISTRY; ENZYME ACTIVATION; EVALUATION; METHODOLOGY; PROTEIN CONFORMATION; PROTEIN DENATURATION; QUALITY CONTROL; TEMPERATURE;

AUTOLYSIS; ELECTROPHORESIS, CAPILLARY; ENDOPEPTIDASES; ENZYME ACTIVATION; PROTEIN CONFORMATION; PROTEIN DENATURATION; QUALITY CONTROL; TEMPERATURE;

EID: 0142245753     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1026040012947     Document Type: Article

References (10)

1. Abraham LD, Breuil C (1995) Factors affecting autolysis of a subtilisin-like serine proteinase secreted by Ophiostoma piceae and identification of the cleavage site. Biochim. Biophys. Acta 1245: 76-84.
2. Bajorath J, Saenger W, Pal GP (1988) Autolysis and inhibition of proteinase K, a subtilisin-related serine proteinase isolated from the fungus Tritirachium album Limber. Biochim. Biophys. Acta 954: 176-182.
3. Chen XL, Zhang YZ, Wang YT, Gao PJ, Luan XW (2001) Psychrophilic protease from a deep-sea psychrotrophilic strain Pseudoaltermnas sp. SM9913. Marine Sci. (China) 25: 4-8.
4. Halfon S, Craik CS (1998) Family S1 of trypsin (clan SA). In: Barrett AJ, Rawlings ND, Woessner JF, eds. Handbook of Proteolytic Enzymes. San Diego: Academic Press, pp. 5-12.
5. Kemp G (1998) Capillary electrophoresis: a versatile family of analytical techniques. Biotechnol. Appl. Biochem. 27: 9-17.
6. Kumura H, Murata S, Hoshino T, Mikawa K, Shimazaki K (1999) Autolysis of the proteinase from Pseudomonas fluorescens J. Dairy Sci. 82: 2078-2083.
7. Leth-Larsen R, Asgeirsson B, Thorolfsson M, Norregaard-Madsen M, Hojrup P (1996) Structure of chymotrypsin variant B from Atlantic cod, Gadus morhua. Biochim. Biophys. Acta 1297: 49-56.
8. Schroeder Leiros HK, Willassen NP, Smalas A (2000) Structural comparison of psychrophilic and mesophilic trypsins-elucidating the molecular basis of cold adaptation. Eur. J. Biochem. 267: 1039-1049.
9. Van den Burg B, Eijsink VG, Vrieng G, Veltman OR, Venema G (1998) Rendering one autolysis site in Bacillus subtilis neutral protease resistant to cleavage reveals a new fission. Biotechnol. Appl. Biochem. 27: 125-132.
10. Varallyay E, Pal G, Patty A, Szilagyi L, Graf L (1998) Two mutations in rat trypsin confer resistance against autolysis. Biochem. Biophys. Res. Commun. 243: 56-60.