Expression and export of a Ruminococcus albus cellulase in Butyrivibrio fibrisolvens through the use of an alternative gene promoter and signal sequence

Canadian Journal of Microbiology

Volumn 49, Issue 6, 2003, Pages 375-382

  Kobayashi, Yasuo ^a   Taguchi, Hidenori ^b   Goto, Takashi N ^b   Koike, Satoshi ^a   Ohmiya, Kunio ^b  

^a HOKKAIDO UNIVERSITY   (Japan)

^b MIE UNIVERSITY   (Japan)

Author keywords

Butyrivibrio fibrisolvens; Cellulase; Gene promoter; Ruminococcus albus; Signal peptide

Indexed keywords

CLONING; CYTOLOGY; GENES; MICROBIOLOGY;

PEPTIDES;

ENZYMES;

CELLULASE; GLUCAN SYNTHASE; XYLAN ENDO 1,3 BETA XYLOSIDASE;

ARTICLE; BUTYRIVIBRIO FIBRISOLVENS; CONTROLLED STUDY; ENZYME ACTIVITY; GENE EXPRESSION; GENE SEQUENCE; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; RUMINOCOCCUS; SIGNAL TRANSDUCTION;

BACTERIAL PROTEINS; BLOTTING, NORTHERN; BLOTTING, WESTERN; BUTYRIVIBRIO; CARBOXYMETHYLCELLULOSE; CELLULASE; CLONING, MOLECULAR; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; PROMOTER REGIONS (GENETICS); PROTEIN SORTING SIGNALS; RECOMBINANT PROTEINS; RNA, MESSENGER; RUMINOCOCCUS; TRANSFORMATION, BACTERIAL; XYLANS;

BUTYRIVIBRIO; BUTYRIVIBRIO FIBRISOLVENS; POSIBACTERIA; RUMINOCOCCUS; RUMINOCOCCUS ALBUS; UNIDENTIFIED SHUTTLE VECTOR;

EID: 0142218828     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/w03-050     Document Type: Article

References (26)

1. Beard, C.E., Hefford, M.A., Forster, R.J., Sontakke, S., Teather, R.M., and Gregg, K. 1995. A stable and efficient transformation system for Butyrivibrio fibrisolvens OB156. Curr. Microbiol. 30: 105-109.
2. Beard, C.E., Gregg, K., Kalmokoff, M., and Teather, R.M. 2000. Construction of a promoter-rescue plasmid for Butyrivibrio fibrisolvens and its use in characterization of a flagellin promoter. Curr. Microbiol. 40: 164-168.
3. Bradford, M.M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
4. Flint, H.J., Thomson, A.M., and Bisset, J. 1988. Plasmid-associated transfer of tetracycline resistance in Bacteroides ruminicola. Appl. Environ. Microbiol. 54: 855-860.
5. Gilmour, M., Mitchell, W.J., and Flint, H.J. 1996. Genetic transfer of lactate-utilizing ability in the rumen bacterium Selenomonas ruminantium. Lett. Appl. Microbiol. 22: 52-56.
6. Gregg, K., Cooper, C.L., Schafer, D.J., Sharpe, H., Beard, C.E., Allen, G., and Xu, J. 1994. Detoxification of the plant toxin fluoroacetate by a genetically modified rumen bacterium. Bio/Technology, 12: 1361-1365.
7. Hefford, M.A., Kobayashi, Y., Allard, S.E., Forster, R.J., and Teather, R.M. 1997. Sequence analysis and characterization of pOM1, a small cryptic plasmid from Butyrivibrio fibrisolvens, and its use in construction of a new family of cloning vectors for Butyrivibrio. Appl. Environ. Microbiol. 63: 1701-1711.
8. Hespell, R.B., and Whitehead, T.R. 1991. Introduction of Tn916 and pAMβ1 into Streptococcus bovis JB1 by conjugation. Appl. Environ. Microbiol. 57: 2710-2713.
9. Karita, S., Sakka, K., and Ohmiya, K. 1997. Cellulosomes, cellulase complexes, of anaerobic microbes: their structure models and functions. In Rumen microbes and digestive physiology in ruminants. Edited by R. Onodera, Y. Sasaki, H. Itabashi, and H. Yano. Japan Scientific Societies Press/S. Karger, Tokyo, Japan/Basel, Switzerland, pp. 47-57.
10. Kawazu, T., Ohta, T., Ito, K., Shibata, M., Kimura, T., Sakka, K., and Ohmiya, K. 1996. Expression of a Ruminococcus albus cellulase gene in tobacco suspension cells. J. Ferment. Bioeng. 82: 205-209.
11. Kobayashi, Y., and Onodera, R. 1999. Application of molecular biology to rumen microbes. Asian-Australas. J. Anim. Sci. 12: 77-83.
12. Kobayashi, Y., Forster, R.J., Hefford, M.A., Teather, R.M., Wakita, M., Ohmiya, K., and Hoshino, S. 1995. Analysis of the sequence of a new cryptic plasmid, pRJF2, from a rumen bacterium of the genus Butyrivibrio: comparison with other Butyrivibrio plasmids and application in the development of a cloning vector. FEMS Microbiol. Lett. 130: 137-144.
13. Kobayashi, Y., Ebata, A., Wakita, M., and Hoshino, S. 1997. Conditions for electrotransforming the ruminal bacterium Butyrivibrio fibrisolvens and some profiles of the transformant. Anim. Sci. Technol. 68: 741-747.
14. Kobayashi, Y., Okuda, N., Matsumoto, M., Inoue, K., Wakita M., and Hoshino, S. 1998. Constitutive expression of a heterologous Eubacterium ruminantium xylanase gene (xynA) in Butyrivibrio fibrisolvens. FEMS Microbiol. Lett. 163: 11-17.
15. Kobayashi, M., Forster, R.J., and Teather, R.M. 2000. Development of a competitive polymerase chain reaction assay for the ruminal bacterium Butyrivibrio fibrisolvens OB156 and its use for tracking an OB156-derived recombinant. FEMS Microbiol. Lett. 188: 185-190.
16. Manarelli, B.M., Evans, S., and Lee, D. 1990. Cloning, sequencing and expression of a xylanase gene from the anaerobic ruminal bacterium Butyrivibrio fibrisolvens. J. Bacteriol. 172: 4247-4254.
17. Miller, G.L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal. Chem. 31: 426-428.
18. Ohmiya, K., Kajino, T., Kato, A., and Shimizu, S. 1989. Structure of a Ruminococcus albus endo-1,4-β-glucanase gene. J. Bacteriol. 171: 6771-6775.
19. Ohmiya, K., Deguchi, H., and Shimizu, S. 1991. Modification of the properties of a Ruminococcus albus endo-1,4-β-glucanase by gene truncation. J. Bacteriol. 173: 636-641.
20. Russell, J.B., and Wilson, D.B. 1988. Potential opportunities and problems for genetically altered rumen microorganisms. J. Nutr. 118: 271-279.
21. Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. Molecular cloning: a laboratory manual. 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
22. Scott, K.P., and Flint, H.J. 1995. Transfer of plasmids between strains of Escherichia coli under rumen conditions. J. Appl. Bacteriol. 78: 189-193.
23. Taguchi, H. 2000. (Mie University.) Unpublished data.
24. Teather, R.M., and Wood, P.J. 1982. Use of Congo red - polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl. Environ. Microbiol. 43: 777-780.
25. Teather, R.M., Hefford, M.A., and Forster, R.J. 1997. Genetics of rumen bacteria. The rumen microbial ecosystem. 2nd ed. Blackie Academic & Professional, London, U.K. pp. 427-466.
26. Xue, G.-P., Johnson, J.S., Bransgrove, K.L., Gregg, K., Beard, C.E., Dalrymple, B.P., Gobius, K., and Aylward, J.H. 1997. Improvement of expression and secretion of a fungal xylanase in the rumen bacterium Butyrivibrio fibrisolvens OB156 by manipulation of promoter and signal sequences. J. Biotechnol. 54: 139-148.