Comparison of flow cytometry and image morphometry in the quantitative analysis of cell population markers in the lymph node of sheep

Veterinary Immunology and Immunopathology

Volumn 94, Issue 3-4, 2003, Pages 177-183

  Gunnes, Gjermund ^a   Valheim, Mette ^b   Press, Charles McL ^a   Tverdal, Aage ^a   Storset, Anne ^a  

^a NORWEGIAN UNIVERSITY OF LIFE SCIENCES   (Norway)

^b NORWEGIAN VETERINARY INSTITUTE   (Norway)

Author keywords

Flow cytometry; Image morphometry; Lymph nodes; Sheep

Indexed keywords

CD2 ANTIGEN; CD8 ANTIGEN; COMPLEMENT COMPONENT C3D RECEPTOR; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2;

ANALYTIC METHOD; ANIMAL TISSUE; ARTICLE; CELL COUNT; CELL POPULATION; CONTROLLED STUDY; FLOW CYTOMETRY; IMMUNOFLUORESCENCE; INTERMETHOD COMPARISON; LAMB; LYMPH NODE CELL; MORPHOMETRICS; NONHUMAN; QUANTITATIVE ANALYSIS; SHEEP; TISSUE DISTRIBUTION; TISSUE STRUCTURE;

OVIS ARIES;

EID: 0142218320     PISSN: 01652427     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-2427(03)00080-1     Document Type: Article

References (17)

1. Akhtar M., Chantziantoniou N. Flow cytometric and quantitative image cell analysis of DNA ploidy in renal chromophobe cell carcinoma. Hum. Pathol. 29:1998;1181-1188.
2. Boeck G. Current status of flow cytometry in cell and molecular biology. Int. Rev. Cytol. 204:2001;239-298.
3. Davis W.C., Marusic S., Lewin H.A., Splitter G.A., Perryman L.E., McGuire T.C., Gorham J.R. The development and analysis of species specific and cross reactive monoclonal antibodies to leukocyte differentiation antigens and antigens of the major histocompatibility complex for use in the study of the immune system in cattle and other species. Vet. Immunol. Immunopathol. 15:1987;337-376.
4. Gunnes G., Press C.M., Tverdal A., Landsverk T. Compartments within the lymph node cortex of calves and adult cattle differ in the distribution of leukocyte populations: an immunohistochemical study using computer-assisted morphometric analysis. Dev. Comp. Immunol. 22:1998;111-123.
5. Hein W.R., Dudler L., Marston W.L., Landsverk T., Young A.J., Avila D. Ubiquitination and dimerization of complement receptor type 2 on sheep B cells. J. Immunol. 161:1998;458-466.
6. Janeway, C., Travers, P., Walport, M., Capra, J.D., 1999. Immunobiology: The Immune System in Health and Disease, 4th ed. Current Biology Publications, London, p. 635.
7. Jeffers M.D., Michie B.A., Oakes S.J., Gillan J.E. Comparison of ploidy analysis by flow cytometry and image analysis in hydatidiform mole and non-molar abortion. Histopathology. 27:1995;415-421.
8. Larsen R.A., Monaghan M.L., Park Y.H., Hamilton M.J., Ellis J.A., Davis W.C. Identification and characterization of monoclonal antibodies reactive with bovine caprine and ovine T-lymphocyte determinants by flow microfluorimetry. Vet. Immunol. Immunopathol. 25:1990;195-208.
9. Liebler E.M., Press C.M., Landsverk T. Lymphocyte subpopulations in jejunal and ileal Peyer's patches of calves with experimental Salmonella dublin infection. Zentralbl. Veterinarmed. 41:1994;113-125.
10. Mackay C.R., Hein W.R., Brown M.H., Matzinger P. Unusual expression of CD2 in sheep: implications for T cell interactions. Eur. J. Immunol. 18:1988;1681-1688.
11. Novelli M., Savoia P., Cambieri I., Ponti R., Comessatti A., Lisa F., Bernengo M.G. Collagenase digestion and mechanical disaggregation as a method to extract and immunophenotype tumour lymphocytes in cutaneous T-cell lymphomas. Clin. Exp. Dermatol. 25:2000;423-431.
12. Ottesen G.L., Christensen I.J., Larsen J.K., Larsen J., Christiansen J., Baldetorp B., Linden T., Hansen B., Andersen J.A. DNA ploidy analysis in breast carcinoma. Comparison of unfixed and fixed tissue analyzed by image and flow cytometry. Anal. Quant. Cytol. Histol. 19:1997;413-422.
13. Press C.M., Halleraker M., Landsverk T. Ontogeny of leukocyte populations in the ileal Peyer's patch of sheep. Dev. Comp. Immunol. 16:1992;229-241.
14. Schraven B. CD2. J. Biol. Regul. Homeost. Agents. 14:2000;223-225.
15. SPSS Inc., 1988. SPSS-X User's Guide, 3rd ed. SPSS Inc., Chicago.
16. Steiner G.E., Ecker R.C., Kramer G., Stockenhuber F., Marberger M.J. Automated data acquisition by confocal laser scanning microscopy and image analysis of triple stained immunofluorescent leukocytes in tissue. J. Immunol. Meth. 237:2000;39-50.
17. Valheim M., Hasvold H., Storset A., Larsen H., Press C. Localisation of CD25+ cells and MHC II+ cells in lymph nodes draining Mycobacterium avium subsp. paratuberculosis vaccination granuloma and the presence of a systemic immune response. Res. Vet. Sci. 73:2002;77.