Analysis of molecular diffusion in ftsK cell-division mutants using laser surgery

EMBO Reports

Volumn 4, Issue 9, 2003, Pages 867-871

  Goksör, Mattias ^a   Diez, Alfredo ^b   Enger, Johan ^a   Hanstorp, Dag ^a   Nyström, Thomas ^b  

^a CHALMERS UNIVERSITY OF TECHNOLOGY   (Sweden)

^b UNIVERSITY OF GOTHENBURG   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

CELL DNA; MEMBRANE PROTEIN; PROPIDIUM IODIDE; PROTEIN FTSK; UNCLASSIFIED DRUG; BACTERIAL DNA;

ARTICLE; BACTERIAL CELL; CARBOXY TERMINAL SEQUENCE; CELL COMPARTMENTALIZATION; CELL DIVISION; CELL MEMBRANE TRANSPORT; CELL MOTION; CELL MUTANT; CELL SEPARATION; CELL ULTRASTRUCTURE; CHROMOSOME SEGREGATION; CONTROLLED STUDY; CYTOKINESIS; CYTOPLASM; ESCHERICHIA COLI; GENETIC ANALYSIS; IMAGING SYSTEM; INTRACELLULAR TRANSPORT; LASER SURGERY; MOLECULAR BIOLOGY; NONHUMAN; PRIORITY JOURNAL; THEORETICAL STUDY; BACTERIAL CHROMOSOME; BACTERIAL MUTATION; DIFFUSION; POLAR BODY;

CELL DIVISION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FLUORESCENT DYES; INDOLES; LASER SURGERY; MEMBRANE PROTEINS; MICRODISSECTION; MICROSCOPY, FLUORESCENCE, MULTIPHOTON; MUTATION; PROPIDIUM; STAINING AND LABELING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0142217459     PISSN: 1469221X     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.embor.embor916     Document Type: Article

References (15)

1. Aussel, L., Barre, F., Aroyo, M., Stasiak, A., Stasiak, A. & Sherratt, D. (2002) FtsK is a DNA motor protein that activates chromosome dimer resolution by switching the catalytic state of the XerC and XerD recombinases. Cell, 108, 195-205.
2. Barre, F.X., Aroyo, M., Colloms, S.D., Helfrich, A., Cornet, F. & Sherratt, D.J. (2000) FtsK functions in the processing of a Holliday junction intermediate during bacterial chromosome segregation. Genes Dev., 14, 2976-2988.
3. Boyle, D.S., Grant, D., Draper, G.C. & Donachie, W.D. (2000) All major regions of FtsK are required for resolution of chromosome dimers. J. Bacteriol., 182, 4124-4127.
4. Diez, A., Farewell, A., Nannmark, U. & Nyström, T. (1997) A mutation in the ftsK gene of Escherichia coli affects cell-cell separation, stationary phase survival, stress adaption and expression of the gene encoding the stress protein UspA. J. Bacteriol., 179, 5878-5883.
5. Diez, A., Gustavsson, N. & Nyström, T. (2000) The universal stress protein A of Escherichia coli is required for resistance to DNA damaging agents and is regulated by a RecA/FtsK-dependent regulatory pathway. Mol. Microbiol., 36, 1494-1503.
6. Ericsson, M., Hanstorp, D., Hagberg, P., Enger, J. & Nyström, T. (2000) Sorting out bacterial viability with optical tweezers. J. Bacteriol., 182, 5551-5555.
7. Fällman, E. & Axner, O. (1997) Design for fully steerable dual-trap optical tweezers. Appl. Opt., 36, 2107-2113.
8. Haugland, R.P. (1996a) in Handbook of Fluorescent Probes and Research Chemicals (ed. Spence, M.), 406-407. Molecular Probes, Inc., Eugene, Oregon, USA.
9. Haugland, R.P. (1996b) in Handbook of Fluorescent Probes and Research Chemicals (ed. Spence, M.), 151. Molecular Probes, Inc., Eugene, Oregon, USA.
10. Haugland, R.P. (1996c) in Handbook of Fluorescent Probes and Research Chemicals (ed. Spence, M.), 372-374. Molecular Probes, Inc., Eugene, Oregon, USA.
11. Liu, G. Draper, G.C. & Donachie, W.D. (1998) FtsK is a bifunctional protein involved in cell division and chromosome localization in Escherichia coli. Mol. Microbiol., 29, 893-903.
12. Sharp, M.D. & Pogliano, K. (1999) An in vivo membrane fusion assay implicates SpoIIIE in the final stages of engulfment during Bacillus subtilis sporulation. Proc. Natl Acad. Sci. USA, 96, 14553-14558.
13. Steiner, W., Liu, G., Donachie, W.D. & Kuempel, P. (1999) The cytoplasmic domain of ftsK protein is required for resolution of chromosome dimers. Mol. Microbiol., 31, 579-583.
14. Wu, L.J & Errington, J. (1994) Bacillus subtilis SpoIIIE protein required for DNA segregation during asymmetric cell division. Science, 264, 572-575.
15. Yu, X.C. Weihe, E.K. & Margolin, W. (1998) Role of the C terminus of FtsK in Escherichia coli chromosome segregation. J. Bacteriol., 180, 6424-6428.