Optimal kinetics for quantification of antigen-induced cytokines in human peripheral blood mononuclear cells by real-time PCR and by ELISA

Journal of Immunological Methods

Volumn 281, Issue 1-2, 2003, Pages 27-35

  Listvanova, Slavka ^a   Temmerman, Stéphane ^a   Stordeur, Patrick ^a   Verscheure, Virginie ^a   Place, Sammy ^a   Zhou, Lin ^a   Locht, Camille ^b   Mascart, Françoise ^a  

^a ERASME HOSPITAL   (Belgium)

^b INSTITUT PASTEUR DE LILLE   (France)

Author keywords

Heparin binding haemagglutinin; Interferon gamma; Interleukin 13; Purified protein derivative; Real time PCR; Tuberculosis

Indexed keywords

ANTIGEN; BETA ACTIN; CYTOKINE; GAMMA INTERFERON; HEMAGGLUTININ; HEPARIN BINDING PROTEIN; INTERLEUKIN 13; INTERLEUKIN 2; INTERLEUKIN 5; TUBERCULIN;

ARTICLE; BACTERIAL INFECTION; CLINICAL ARTICLE; CONTROLLED STUDY; CYTOKINE PRODUCTION; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; HUMAN CELL; KINETICS; MONONUCLEAR CELL; MYCOBACTERIUM TUBERCULOSIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STIMULATION; TH1 CELL; TH2 CELL;

MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS;

EID: 0142216542     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(03)00267-9     Document Type: Article

References (23)

1. Blaschke V., Reich K., Blaschke S., Zipprich S., Neumann C. Rapid quantification of proinflammatory and chemoattractant cytokine expression in small tissue samples and monocyte-derived dendritic cells: validation of a new real-time RT-PCR technology. J. Immunol. Methods. 246:2000;79.
2. Byl B., Gérard M., Libin M., Clumeck N., Goldman M., Mascart F. Single cell analysis of antigen-specific T cell responses in HIV-infected individuals. Cell. Immunol. 192:1999;107.
3. ElGhazali G.E.B., Paulie S., Andersson G., Hansson Y., Holmquist G., Sun J.B., Olsson T., Ekre H.P., Troye-Blomberg M. Number of interleukin-4 and interferon-γ-secreting human T cells reactive with tetanus toxoid and the mycobacterial antigen PPD or phytohemagglutinin: distinct response profiles depending on the type of antigen used for activation. Eur. J. Immunol. 23:1993;2740.
4. Esnault S., Benbernou N., Lavaud F., Shin H.C., Potron G., Guenounou M. Differential spontaneous expression of mRNA for IL-4, IL-10, IL-13, IL-2 and interferon-gamma (IFN-gamma) in peripheral blood mononuclear cells (PBMC) from atopic patients. Clin. Exp. Immunol. 103:1996;111.
5. Fan J., Nishanian P., Breen E.C., McDonald M., Fahey J.L. Cytokine gene expression in normal human lymphocytes in response to stimulation. Clin. Diagn. Lab. Immunol. 5:1998;335.
6. Favre N., Bordmann G., Rudin W. Comparison of cytokine measurements using ELISA, ELISPOT and semi-quantitative RT-PCR. J. Immunol. Methods. 204:1997;57.
7. Fernandez V., Andersson J., Andersson U., Troye-Blomberg M. Cytokine synthesis analysed at the single-cell level before and after revaccination with tetanus toxoid. Eur. J. Immunol. 24:1994;1808.
8. Giulietti A., Overbergh L., Valckx D., Cecallonne B., Bouillon R., Mathieu C. An overview of real-time quantitative PCR: applications to quantify cytokine gene expression. Methods. 25:2001;386.
9. Guhaniyogi J., Brewer G. Regulation of mRNA stability in mammalian cells. Gene. 265:2002;11.
10. Härtel C., Bein G., Müller-Steinhardt M., Klüter H. Ex vivo induction of cytokine mRNA expression in human blood samples. J. Immunol. Methods. 249:2001;63.
11. Kabilan L., Andersson G., Lolli F., Ekre H.P., Olsson T., Troye-Blomberg M. Detection of intracellular expression and secretion of interferon-γ at the single-cell level after activation of human T cells with tetanus toxoid in vitro. Eur. J. Immunol. 20:1990;1085.
12. Kruse N., Moriabadi N.F., Toyka K.V., Rieckmann P. Characterization of early immunological responses in primary cultures of differentially activated human peripheral mononuclear cells. J. Immunol. Methods. 247:2001;131.
13. Ledur A., Fitting C., David B., Hamberger C., Cavaillon J.M. Variable estimates of cytokine levels produced by commercial ELISA kits: results using international cytokine standards. J. Immunol. Methods. 186:1995;171.
14. Lucey D.R., Clerici M., Shearer G.M. Type 1 and type 2 cytokine dysregulation in human infectious, neoplastic, and inflammatory diseases. Clin. Microbiol. Rev. 9:1996;532.
15. Masungi C., Temmerman S., Van Vooren J.P., Drowart A., Pethe K., Menozzi F., Locht C., Mascart F. Differential T and B cell responses against Mycobacterium tuberculosis heparin-binding hemagglutinin adhesin in infected healthy individuals and patients with tuberculosis. J. Infect. Dis. 185:2002;513.
16. Menozzi F.D., Rouse J.H., Alavi M., Laude-Sharp M., Muller J., Bischoff R., Brennan M.J., Locht C. Identification of a heparin-binding hemagglutinin present in mycobacteria. J. Exp. Med. 184:1996;993.
17. Overbergh L., Valckx D., Waer M., Mathieu C. Quantification of murine cytokine mRNAs using real time quantitative reverse transcriptase PCR. Cytokine. 11:1999;305.
18. O'Garra A., Vieira P. Polymerase chain reaction for detection of cytokine gene expression. Curr. Opin. Immunol. 4:1992;211.
19. Raupach B., Kaufmann S.H.E. Immune responses to intracellular bacteria. Curr. Opin. Immunol. 13:2001;417.
20. Romagnani S. The Th1/Th2 paradigm. Immunol. Today. 18:1997;263.
21. Rowe J., Macaubas C., Monger T., Holt B.J., Harvey J., Poolman J.T., Loh R., Sly P.D., Holt P.G. Heterogeneity in diphtheria-tetanus-acellular pertussis vaccine-specific cellular immunity during infancy: relationship to variations in the kinetics of postnatal maturation of systemic Th1 function. J. Infect. Dis. 184:2001;80.
22. Seah G.T., Scott G.M., Rook G.A.W. Type 2 cytokine gene activation and its relationship to extent of disease in patients with tuberculosis. J. Infect. Dis. 181:2000;385.
23. Stordeur P., Poulin L., Cracium L., Zhou L., Schandené L., de Lavarelle A., Goriely S., Goldman M. Cytokine mRNA quantification by real-time PCR. J. Immunol. Methods. 259:2002;55.