A continuous spectrophotometric assay for the determination of diamondback moth esterase activity

Archives of Insect Biochemistry and Physiology

Volumn 54, Issue 2, 2003, Pages 68-76

  He, Xiaodun ^a  

^a KIKA DE LA GARZA SUBTROPICAL AGRICULTURAL RESEARCH CENTER   (United States)

Author keywords

Detoxification enzyme; Esterase; Naphthol; Naphthyl acetate; Spectrophotometric assay

Indexed keywords

AZADIRACHTA INDICA; HEXAPODA; INSECTA; LEPIDOPTERA; PLUTELLA XYLOSTELLA;

1 NAPHTHOL; 1-NAPHTHOL; AZADIRACHTIN; ENZYME INHIBITOR; ESTERASE; LIMONOID; NAPHTHOL DERIVATIVE; NAPHTHYL ACETATE; POTASSIUM; SODIUM;

ANIMAL; ARTICLE; BINDING COMPETITION; CHEMISTRY; DRUG ANTAGONISM; ENZYMOLOGY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; KINETICS; METABOLISM; METHODOLOGY; MOTH; PH; TEMPERATURE; ULTRAVIOLET SPECTROPHOTOMETRY;

ANIMALS; BINDING, COMPETITIVE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENZYME INHIBITORS; ESTERASES; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; LIMONINS; MOTHS; NAPHTHOLS; POTASSIUM; SODIUM; SPECTROPHOTOMETRY, ULTRAVIOLET; TEMPERATURE;

EID: 0142182224     PISSN: 07394462     EISSN: None     Source Type: Journal    
DOI: 10.1002/arch.10103     Document Type: Article

References (19)

1. Aldridge WN, Reinter E. 1972. Enzyme inhibitors as substrates: Interaction of esterases with esters of organo-phosphors and carbamic acids. In: Neuberger A, Tatum EL, editors. Frontiers of biology, vol. 26. Amsterdam: North-Holland.
2. Anspaugh DD, Rose RL, P Koehler G, Hodgson E, Roe RM. 1994. Multiple mechanisms of pyrethroid resistance in the German cockroach, Blattella germanica (L.). Pestic Biochem Physiol 50:138-148.
3. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254.
4. Dary O, Georghiou GP, Parsons E, Pasteur N. 1990. Microplate adaptation of Gomori's assay for quantitative determination of esterase activity in single insects. J Econ Entomol 83:2187-2192.
5. Dauterman WC. 1982-83. The role of hydrolaseses in insecticide metabolism and the toxicological significance of the metabolites. J Toxicol-Clin Toxicol 196/197:623-635.
6. Dowd PF, Smith CM, Sparks TC. 1983. Detoxification of plant toxins by insects. Insect Biochem 13:453-468.
7. Elzen GW, James RR. 2002. Responses of Plutella xylostella and Coleomegilla maculata to selected insecticides in a residual insecticide bioassay. Southwest Entomol 27:149-153.
8. Gomori G. 1953. Human esterase. J Lab Clin Med 42:445-453.
9. Grant DF, Bender DM, Hammock BD. 1989. Quantitative kinetic assays for glutathione S-tranferase and esterase in individual mosquitoes using an EIA reader. Insect Biochem 19:741-751.
10. Ishaaya I. 1993. Insect detoxifying enzymes: Their importance in pesticide synergism and resistance. Arch Insect Biochem Physiol 22:263-276.
11. Johnston KJ, Ashford AE. 1980. A simultaneous-coupling azo dye method for the quantitative assay of esterase using alpha-naphthyl acetate as substrate. Histochem J 12:221-234.
12. Kapin MA, Ahmad S. 1980. Esterases in larval tissues of gypsy moth, Lymantria dispar (L.): optimum assay conditions, quantification and characterization. Insect Biochem 10:331-337.
13. Maa WCJ, Chuang ML. 1983. Esterase of diamond-back moth (Plutella xylostella L.). I. Enzymatic properties of larval esterases. Bull Inst Zool Academia Sinica 22:123-131.
14. Mastropaolo W, Yourno J. 1981. An ultraviolet spectrophotometric assay for α-naphthyl acetate and α-naphthyl butyrate esterase. Anal Biochem 115:188-193.
15. Mukherjee SN, Sharma RN. 1996. Azadirachtin induced changes in feeding, dietary utilization and midgut carboxylesterase activity of the final instar larvae of Spodoptera litura (Farbicicus) (Lepidoptera: Noctuidae). J Environ Sci Health B31:1307-1319.
16. Rudnicka, M, Kochman, M. 1984. Purification of the juvenile hormone esterase from the haemolymph of the wax moth Galleria mellonell (Lepidoptera). Insect Biochem 14:189-198.
17. Talekar NS, Shelton AM. 1993. Biology, ecology, and management of the diamondback moth. Annu Rev Entomol 38:275-301.
18. Terriere, LC. 1984. Introduction of detoxification enzymes in insects. Ann Rev Entomol 29:71-88.
19. van Asperen K. 1962. A study of housefly esterase by means of a sensitive colorimetric method. J Ins Physiol 8:401-416.