A new approach for the detection of multiple protein kinases using monoclonal antibodies directed to the highly conserved region of protein kinases

Analytical Biochemistry

Volumn 322, Issue 2, 2003, Pages 215-224

  Kameshita, Isamu ^a   Tsuge, Toshiyuki ^a   Kinashi, Tomoko ^a   Kinoshita, Shun ^a   Sueyoshi, Noriyuki ^a   Ishida, Atsuhiko ^b   Taketani, Shigeru ^c   Shigeri, Yasushi ^d   Tatsu, Yoshiro ^d   Yumoto, Noboru ^d   Okazaki, Katsuichiro ^a  

^a KAGAWA UNIVERSITY   (Japan)

^b ASAHIKAWA MEDICAL COLLEGE   (Japan)

^c KYOTO INSTITUTE OF TECHNOLOGY   (Japan)

^d NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

cDNA library; Expression cloning; Immunoprecipitation; Immunoscreening; In gel protein kinase assay; Protein kinase family

Indexed keywords

AMINO ACIDS; CHEMICAL DETECTION; CLONE CELLS; CLONING; ENZYMES; MONOCLONAL ANTIBODIES; PEPTIDES;

CDNA LIBRARIES; EXPRESSION CLONING; IMMUNO-SCREENING; IMMUNOPRECIPITATIONS; PROTEIN KINASE;

CALMODULIN;

ANTIGEN; CALMODULIN DEPENDENT PROTEIN KINASE II; CALMODULIN DEPENDENT PROTEIN KINASE IV; CELL EXTRACT; CYCLIC AMP DEPENDENT PROTEIN KINASE; CYSTEINYLVALYLVALYLHISTIDYLARGINYLASPARTYLLEUCYLLYSYLPROLYLGLUTAMYLASPARAGI NYLLEUCYLLEUCYLLEUCYLALANYLSERINE; ENZYME ANTIBODY; ENZYME VARIANT; MITOGEN ACTIVATED PROTEIN KINASE; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY M1C; MONOCLONAL ANTIBODY M8C; PROTEIN KINASE; PROTEIN SERINE THREONINE KINASE; SYNTHETIC PEPTIDE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ANTIBODY PRODUCTION; ARTICLE; CONTROLLED STUDY; CROSS REACTION; DNA LIBRARY; ENZYME ASSAY; IMMUNIZATION; IMMUNOPRECIPITATION; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FAMILY; WESTERN BLOTTING;

EID: 0142164914     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2003.08.014     Document Type: Article

References (29)

1. Manning G., Plowman G.D., Hunter T., Sudarsanam S. Evolution of protein kinase signaling from yeast to man. Trends Biochem. Sci. 27:2002;514-520.
2. Hanks S.K., Hunter T. The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification. FASEB J. 9:1995;576-596.
3. Kameshita I., Taketani S., Ishida A., Fujisawa H. Detection of a variety of Ser/Thr protein kinases using a synthetic peptide with multiple phosphorylation sites. J. Biochem. 126:1999;991-995.
4. Hanks S.K. Homology probing: Identification of cDNA clones encoding members of the protein-serine kinase family. Proc. Natl. Acad. Sci. USA. 84:1987;388-392.
5. Kentrup H., Becker W., Heukelbach J., Wilmes A., Schurmann A., Huppertz C., Kainulainen H., Joost H.-G. Dyrk, a dual specificity protein kinase with unique structural features whose activity is dependent on tyrosine residues between subdomains VII and VIII. J. Biol. Chem. 271:1996;3488-3495.
6. Becker W., Weber Y., Wetzel K., Eirmbter K., Tejedor F.J., Joost H.-G. Sequence characteristics, subcellular localization, and substrate specificity of DYRK-related kinases, a novel family of dual specificity protein kinases. J. Biol. Chem. 273:1998;25893-25902.
7. Kornbluth S., Paulson K.E., Hanafusa H. Novel tyrosine kinase identified by phosphotyrosine antibody screening of cDNA libraries. Mol. Cell. Biol. 8:1988;5541-5544.
8. Lindberg R.A., Thompson D.P., Hunter T. Identification of cDNA clones that code for protein-tyrosine kinases by screening expression libraries with antibodies against phosphotyrosine. Oncogene. 3:1988;629-633.
9. Letwin K., Yee S.-P., Pawson T. Novel protein-tyrosine kinase cDNA related to fps/fes and eph cloned using anti-phosphotyrosine antibody. Oncogene. 3:1988;621-627.
10. Pasquale E.B., Singer S.J. Identification of a developmentally regulated protein-tyrosine kinase by using anti-phosphotyrosine antibodies to screen a cDNA expression library. Proc. Natl. Acad. Sci. USA. 86:1989;5449-5453.
11. Okuno S., Fujisawa H. Stabilization, purification and crystallization of catalytic subunit of cAMP-dependent protein kinase from bovine heart. Biochim. Biophys. Acta. 1038:1990;204-208.
12. Woodgett J.R., Hunter T. Isolation and characterization of two distinct forms of protein kinase C. J. Biol. Chem. 262:1987;4836-4843.
13. 2+/ calmodulin. J. Biochem. 115:1994;1075-1082.
14. Kameshita I., Fujisawa H. Preparation and characterization of calmodulin-dependent protein kinase IV (CaM-kinase IV) free of CaM-kinase IV kinase from rat cerebral cortex. J. Biochem. 117:1995;85-90.
15. Kameshita I., Fujisawa H. Detection of protein kinase activities toward oligopeptides in sodium dodecyl sulfate-polyacrylamide gel. Anal. Biochem. 237:1996;198-203.
16. Miyano O., Kameshita I., Fujisawa H. Purification and characterization of a brain-specific multifunctional calmodulin-dependent protein kinase from rat cerebellum. J. Biol. Chem. 267:1992;1198-1203.
17. Bensadoun A., Weinstein D. Assay of proteins in the presence of interfering materials. Anal. Biochem. 70:1976;241-250.
18. Kennett R.H. Cell fusion. Methods Enzymol. 58:1979;345-359.
19. Hawkes R., Niday E., Gordon J. A dot-immunobinding assay for monoclonal and other antibodies. Anal. Biochem. 119:1982;142-147.
20. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
21. Kameshita I., Fujisawa H. A sensitive method for detection of calmodulin-dependent protein kinase II activity in sodium dodecyl sulfate-polyacrylamide gel. Anal. Biochem. 183:1989;139-143.
22. Young R.A., Davis R.W. Efficient isolation of genes by using antibody probes. Proc. Natl. Acad. Sci. USA. 80:1983;1194-1198.
23. Tucker K.E., Massello L.K., Gao L., Barber T.J., Hebert M.D., Chan E.K.L., Matera A.G. Structure and characterization of the murine p80 coilin gene, Coil. J. Struct. Biol. 129:2000;269-277.
24. Park S.-H., Weinberg R.A. A putative effector of Ral has homology to Rho/Rac GTPase activating proteins. Oncogene. 11:1995;2349-2355.
25. 2+/calmodulin-dependent protein kinase II: the role of structure and autoregulation in cellular function. Annu. Rev. Biochem. 71:2002;473-510.
26. Burgess H.A., Martinez S., Reiner O. KIAA0369, Doublecortin-like kinase, is expressed during brain development. J. Neurosci. Res. 58:1999;567-575.
27. Lerner R.A. Tapping the immunological repertoire to produce antibodies of predetermined specificity. Nature. 299:1982;592-596.
28. Knighton D.R., Zheng J., Ten-Eyck L.F., Ashford V.A., Xuong N.-H., Taylor S.S., Sowadski J.M. Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase. Science. 253:1991;407-414.
29. 2+ adenylyl imidodiphosphate and inhibitor peptide PKI(5-24). EMBO J. 12:1993;849-859.