Purification and characterization of extracellular temperature-stable serine protease from Aeromonas hydrophila

Journal of Microbiology

Volumn 41, Issue 3, 2003, Pages 207-211

  Cho, Soo Jin ^a   Park, Jong Ho ^a   Park, Seong Joo ^a   Lim, Jong Soon ^a   Kim, Eung Ho ^b   Cho, Yeon Jae ^c   Shin, Kwang Soo ^a  

^a Daejeon University   (South Korea)

^b Hong Ik University   (South Korea)

^c Seil Technology Co LTD   (South Korea)

Author keywords

Aeromonas hydrophila; Characterization; Extracellular protease; Purification

Indexed keywords

BENZYLSULFONYL FLUORIDE; CALCIUM; EDETIC ACID; MAGNESIUM; PEPSTATIN; PHENANTHROLINE; SEPHAROSE; SERINE PROTEINASE; ZINC;

ACID PRECIPITATION; AEROMONAS HYDROPHILA; ARTICLE; CHROMATOGRAPHY; ELECTROPHORETIC MOBILITY; ENZYME ACTIVITY; ENZYME DEGRADATION; ENZYME PURIFICATION; ENZYME SUBUNIT; HEAT TREATMENT; HYDROLYSIS; PH MEASUREMENT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; THERMOSTABILITY;

AEROMONAS; AEROMONAS HYDROPHILA;

EID: 0142056277     PISSN: 12258873     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Allan, B. and R.M.W. Stevenson. 1981. Extracellular virulence factor of Aeromonas hydrophila in fish infections. Can. J. Microbiol. 27, 1114-1122.
2. Bradford, M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
3. Dahle, H.K. 1971. The purification and some properties of two Aeromonas proteinases. Acta Pathol. Microbiol. Immunol. Scand. Sect B 79, 726-738.
4. Gross, R. and N.W. Coles. 1969. A proteinase produced by Aeromonas hydrophila. Aust. J. Sci. 31, 330-331.
5. Hsu, T.C., W.D. Waltman, and E.B. Shots. 1981. Correlation of extracellular enzymatic activity and biochemical characteristics with regard to virulence of Aeromonas hydrophila. Dev. Biol. Stand. 49, 101-111.
6. Huang, Q., Y. Peng, X. Li, H. Wang, and Y. Zhang. 2003. Purification and characterization of an extracellular alkaline serine protease with dehairing function from Bacillus pumilus. Curr. Microbiol. 46, 169-173.
7. Kanai, K. and H. Wakabayashi. 1984. Purification and some properties of proteases from Aeromonas hydrophila. Bull. Jpn. Soc. Sci. Fish. 40, 1367-1374.
8. Kim, Y.K., J.H. Bae, B.K. Oh, W.H. Lee, and J.W. Choi. 2002. Enhancement of proteolytic enzyme activity excreted from Bacillus stearothermophilus for a thermophilic aerobic digestion process. Bioresource Technol. 82, 157-164.
9. Laemmli, U.K. 1970. Cleavage associated with the maturation of the head of bacteriophage T4. Nature 227, 680-685.
10. Leung, Y.K. and R.M. Stevenson. 1988. Charcteristics and distribution of extracellular proteases from Aeromonas hydrophila. J. Gen. Microbiol. 134, 151-160.
11. Loewy, A.G., U.V. Santer, M. Wieczorek, J.K. Blodge, S.W. Jones, and J.C. Cheranis. 1993. Purification and characterization of a novel zinc-proteinase from cultures of Aeromonas hydrophila. J. Biol. Chem. 268, 9071-9078.
12. Neuhoff, V., N. Arold, D. Taube, and W. Ehrhardt. 1988. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie brilliant blue G-250 and R-250. Electrophoresis 9, 255-262.
13. Nieto, T.P. and E. Ellis. 1986. Characterization of extracellular metallo- and serine-proteases of Aeromonas hydrophila strain B51. J. Gen. Microbiol. 132, 1975-1979.
14. Pemberton, J.M., S.P. Kidd, and R. Schmidt. 1997. Secreted enzymes of Aeromonas. FEMS Microbiol. Lett. 152, 1-10.
15. Rivero, O., J. Anguita, C. Paniagua, and G. Naharro. 1990. Molecular cloning and characterization of an extracelluar protease gene from Aeromonas hydrophila. J. Bacteriol. 172, 3905-3908.
16. Sarath, G., R.S.D.L. Motte, and F.W. Wagner. 1989. Protease assay methods, p. 25-55. In R.J. Beynon and J.S. Bond (eds.), Proteolytic enzymes: A practical approach. IRL Press, Oxford, New York, Tokyo.