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note
-
The original cover glass we used has Si-OH groups exposed on the surface. We treated the cover glass with a mixture of (3-mercaptopropyl)- trimethoxysilane and acetone (1:9 by volume) overnight to make the thiol-presenting glass surface. Similarly, we used a mixture of 10% 3-(aminopropyl)triethoxysilane and 90% acetone by volume to make the amino-presenting glass surface. After silanization, the glasses were washed with acetone, acetone/ethanol, ethanol, and DI water. Then the glasses were dried overnight. The treated glasses were used within 48 h for this experiment.
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25
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0142102006
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note
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In this experiment, the particles were suspended in DI water of pH 7. It requires the presence of a strong acid or base catalyst for carboxylic and hydroxyl groups to react and form esters. Usually, heating is required for acid-catalyzed esterification.
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0142101993
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note
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This ambiguity can be clarified by adjusting the experimental setup. For example, an objective lens with higher NA can be substituted into the optical system.
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