Isolation of scorpion (Androctonus amoreuxi) putative alpha neurotoxins and parallel cloning of their respective cDNAs from a single sample of venom

Regulatory Peptides

Volumn 115, Issue 2, 2003, Pages 115-121

  Chen, Tianbao ^a   Folan, Ronan ^a   Kwok, HangFai ^a   O'Kane, Edmund J ^a   Bjourson, Anthony J ^a   Shaw, Chris ^a  

^a UNIVERSITY OF ULSTER   (United Kingdom)

Author keywords

cDNA cloning; Neurotoxin; Precursor; Scorpion; Venom

Indexed keywords

ALPHA TOXIN; COMPLEMENTARY DNA; NEUROTOXIN; SCORPION VENOM;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; FREEZE DRYING; GENOME ANALYSIS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ISOLATION; SCORPION; STRUCTURE ANALYSIS; TOXIN ANALYSIS; TOXIN STRUCTURE;

ANDROCTONUS; ANDROCTONUS AMOREUXI; ANIMALIA; BUTHIDA; SCORPIONES;

EID: 0142043336     PISSN: 01670115     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-0115(03)00146-0     Document Type: Article

References (26)

1. Lourenco W.R. Diversity and endemism in tropical versus temperate scorpion communities. Biogeographica. 70:1994;155-160.
2. Srinivasan K.N., Gopalakrishnakone P., Tan P.T., Chew K.C., Cheng B., Kini R.M.et al. SCORPION, a molecular database of scorpion toxins. Toxicon. 40:2002;23-31.
3. Briggs D.E.G. Scorpions take to the water. Nature. 326:1987;645-646.
4. Zlotkin E., Eitan M., Bindokas V.P., Adams M.E., Moyer M., Burkhart W.et al. Functional duality and structural uniqueness of depressant insect-selective neurotoxins. Biochemistry. 30:1991;4814-4821.
5. Martin Eauclaire M.F., Couraud F. Scorpion neurotoxins: effects and mechanisms. Chang L.W., Dyer R.S. Handbook of neurotoxicology. 1995;683-716 Marcel Dekker, New York.
6. Possani L.D. Structure of scorpion toxins. Tu A.T. Handbook of natural toxins. vol. 2:1984;513-550 Marcel Dekker, New York.
7. Rochat H., Bernard P., Couraud F. Scorpion toxins: chemistry and mode of action. Ceccarelli B., Clementi F. Advances in cytopharmacology. vol. 3:1979;325-334 Raven Press, New York.
8. + channels by a novel scorpion toxin. Nature (Lond.). 296:1982;90-91.
9. Debin J.A., Maggio J.E., Strichartz G.R. Purification and characterization of chlorotoxin, a chloride channel ligand from the venom of the scorpion. Am. J. Physiol. 264:1993;C361-C369.
10. Valdivia H., Possani L.D. Peptide toxins as probes of ryanodine receptor. Trends Cardiovasc. Med. 8:1998;111-118.
11. Loret E.P., Mansuelle P., Rochat H., Granier C. Neurotoxins active on insects: amino acid sequences, chemical modifications and secondary structure estimation by circular dichroism of toxins from the scorpion Androctonus australis Hector. Biochemistry. 29:1990;1492-1501.
12. Bougis P.E., Rochat H., Smith L.A. Precursors of Androctonus australis scorpion neurotoxins. J. Biol. Chem. 264:1989;19259-19265.
13. Nakagawa Y., Lee Y.M., Lehmberg E., Herrmann R., Moskowitz H., Jones A.D.et al. Anti-insect toxin (AaIT5) from Androctonus australis. Eur. J. Biochem. 246:1997;496-501.
14. Gordon D., Savarin P., Gurevitz M., Zinn-Justin S. Functional anatomy of scorpion toxins affecting sodium channels. J. Toxicol., Toxin Rev. 17:1998;131-159.
15. + channels, isolated from Heterometrus spinnifer (Scorpionidae) venom. Biochem. J. 328:1997;321-327.
16. +-channels. Eur. J. Biochem. 264:1999;287-300.
17. Lourenco W. Peut-on parler d'une biogeography du scorpionisme. C. R. Soc. Biogeogr. 64:1988;137-143.
18. Lourival D., Possani E.M., Miguel C., Francisco B., Baltazar B. Peptides and genes coding for scorpion toxins that affect ion-channels. Biochimie. 82:2000;861-868.
19. Ben A.Z., Boursot P., Said K., Lagnel J., Navajas M. Comparison of ribosomal ITS regions among Androctonus spp. Scorpions (Buthidae) from Tunisia. J. Med. Entomol. 1:2000;787-790.
20. Zhu S., Li W. Precursors of three unique cysteine-rich peptides from the scorpion Buthus martensii Karsch. Comp. Biochem. Physiol., B. 131:2002;749-756.
21. +-channels blocking peptides from scorpions of the genus Centruroides. FEBS Lett. 532:2002;121-126.
22. Ye J.G., Wang C.Y., Li Y.J., Tan Z.Y., Yan Y.P., Li C.et al. Purification, cDNA cloning and function assessment of BmK abT, a unique component from the Old World scorpion species. FEBS Lett. 479:2000;136-140.
23. Froy O., Zilberberg N., Gordon D., Turkov M., Gilles N., Stankiewicz M. et al. The putative bioactive surface of insect-selective scorpion excitatory neurotoxins. J. Biol. Chem. 274:1992;5769-5776.
24. Guan R.J., Wang C.G., Wang M., Wang D.C. A depressant insect toxin with a novel analgesic effect from scorpion Buthus martensii Karsch. Biochim. Biophys. Acta. 1549:2001;9-18.
25. Zhou X.H., Yang D., Zhang J.H., Liu C.M., Lei K.J. Purification and N-terminal partial sequence of anti-epilepsy peptide from venom of the scorpion BmK. Biochem. J. 257:1989;509-517.
26. Ceard B., Martin-Eauclaire M., Bougis P.E. Evidence for a position-specific deletion as an evolutionary link between long- and short-chain scorpion toxins. FEBS Lett. 494:2001;246-248.